Abstract:
:The S box transcription termination control system regulates expression of genes involved in methionine metabolism. Expression of the S box regulon, comprised of 11 transcriptional units in Bacillus subtilis, is induced in response to starvation for methionine. We previously demonstrated that S-adenosylmethionine (SAM) is the molecular effector sensed by the S box leader RNAs during transcription. A secondary structure model for S box leader RNAs was developed based on conservation of primary sequence elements and sequence covariation in helical domains. Covariation of nucleotides in two distantly spaced unpaired regions in the S box leader RNAs suggested that these two domains might interact in the RNA tertiary structure. In this study, site-directed mutagenesis of the covarying residues in two B. subtilis S box leader sequences was employed to test the hypothesis that base-pairing between these regions may be important. The effect of these mutations on in vivo expression, transcription termination in vitro, SAM binding, and leader RNA structure strongly supported the model that interaction between these two regions plays a key role in S box leader function. This provides the first insight into the three-dimensional arrangement of structural elements within the S box RNAs.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
McDaniel BA,Grundy FJ,Henkin TMdoi
10.1111/j.1365-2958.2005.04740.xsubject
Has Abstractpub_date
2005-08-01 00:00:00pages
1008-21issue
4eissn
0950-382Xissn
1365-2958pii
MMI4740journal_volume
57pub_type
杂志文章abstract::Escherichia coli and Salmonella can use chitin-derived oligosaccharides as carbon and nitrogen sources. Chitosugars traverse the outer membrane through a dedicated chitoporin, ChiP, and are transported across the cytoplasmic membrane by the chitobiose transporter (ChbBCA). Previous work revealed that synthesis of the ...
journal_title:Molecular microbiology
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更新日期:2005-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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