Tag-mediated fractionation of yeast ribosome populations proves the monomeric organization of the eukaryotic ribosomal stalk structure.


:The analysis of the not well understood composition of the stalk, a key ribosomal structure, in eukaryotes having multiple 12 kDa P1/P2 acidic protein components has been approached using these proteins tagged with a histidine tail at the C-terminus. Tagged Saccharomyces cerevisiae ribosomes, which contain two P1 proteins (P1 alpha and P1 beta) and two P2 proteins (P2 alpha and P2 beta), were fractionated by affinity chromatography and their stalk composition was determined. Different yeast strains expressing one or two tagged proteins and containing either a complete or a defective stalk were used. No indication of protein dimers was found in the tested strains. The results are only compatible with a stalk structure containing a single copy of each one of the four 12 kDa proteins per ribosome. Ribosomes having an incomplete stalk are found in wild-type cells. When one of the four proteins is missing, the ribosomes do not carry the three remaining proteins simultaneously, containing only two of them distributed in pairs made of one P1 and one P2. Ribosomes can carry two, one or no acidic protein pairs. The P1 alpha/P2 beta and P1beta/P2 alpha pairs are preferentially found in the ribosome, but they are not essential either for stalk assembly or function.


Mol Microbiol


Molecular microbiology


Guarinos E,Santos C,Sánchez A,Qiu DY,Remacha M,Ballesta JP




Has Abstract


2003-10-01 00:00:00














  • Characterization of a new cytotoxin that contributes to Staphylococcus aureus pathogenesis.

    abstract::Staphylococcus aureus is an important pathogen that continues to be a significant global health threat because of the prevalence of methicillin-resistant S. aureus strains (MRSA). The pathogenesis of this organism is partly attributed to the production of a large repertoire of cytotoxins that target and kill innate im...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dumont AL,Nygaard TK,Watkins RL,Smith A,Kozhaya L,Kreiswirth BN,Shopsin B,Unutmaz D,Voyich JM,Torres VJ

    更新日期:2011-02-01 00:00:00

  • Function of bacteriophage G7C esterase tailspike in host cell adsorption.

    abstract::Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs show a great variability in their shapes, sizes, and location on the particle. Some RBPs are known to depolymerize surface polysaccharides of...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Prokhorov NS,Riccio C,Zdorovenko EL,Shneider MM,Browning C,Knirel YA,Leiman PG,Letarov AV

    更新日期:2017-08-01 00:00:00

  • Sigma28 RNA polymerase regulates hctB, a late developmental gene in Chlamydia.

    abstract::Chlamydia is predicted to encode two alternative sigma factors that could provide a mechanism for the regulation of gene expression via alternative forms of RNA polymerase. We have demonstrated that sigma 28, one of these alternative sigma factors, is transcriptionally active. Chlamydial sigma 28 RNA polymerase was re...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Yu HH,Tan M

    更新日期:2003-10-01 00:00:00

  • Selective inactivation of SIDER2 retroposon-mediated mRNA decay contributes to stage- and species-specific gene expression in Leishmania.

    abstract::Despite their high genomic synteny, the Leishmania major and Leishmania infantum species exhibit extensive differences in mRNA expression patterns throughout the parasite's development. Yet, the underlying mechanisms for this species-specific differential gene expression are largely unknown. Here we report that Short ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Müller M,Padmanabhan PK,Papadopoulou B

    更新日期:2010-07-01 00:00:00

  • Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans.

    abstract::The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence in the human fungal pathogen Candida albicans. It has long been thought that the PKA catalytic subunit is essential for cell viability in this fungus. Paradoxically, the single adenylyl c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cao C,Wu M,Bing J,Tao L,Ding X,Liu X,Huang G

    更新日期:2017-07-01 00:00:00

  • Menaquinone synthesis is critical for maintaining mycobacterial viability during exponential growth and recovery from non-replicating persistence.

    abstract::Understanding the basis of bacterial persistence in latent infections is critical for eradication of tuberculosis. Analysis of Mycobacterium tuberculosis mRNA expression in an in vitro model of non-replicating persistence indicated that the bacilli require electron transport chain components and ATP synthesis for surv...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dhiman RK,Mahapatra S,Slayden RA,Boyne ME,Lenaerts A,Hinshaw JC,Angala SK,Chatterjee D,Biswas K,Narayanasamy P,Kurosu M,Crick DC

    更新日期:2009-04-01 00:00:00

  • Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.

    abstract::Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully ass...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pizza M,Domenighini M,Hol W,Giannelli V,Fontana MR,Giuliani MM,Magagnoli C,Peppoloni S,Manetti R,Rappuoli R

    更新日期:1994-10-01 00:00:00

  • Pili with strong attachments: Gram-positive bacteria do it differently.

    abstract::Bacteria attach to their appropriate environmental niche by using adhesins. To maximize their contact with the environment, adhesins are often present on the ends of long hairlike structures called pili. Recently, attention has focused on pili of Gram-positive bacteria because they may be vaccine candidates in importa...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Scott JR,Zähner D

    更新日期:2006-10-01 00:00:00

  • Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore.

    abstract::Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus. The VirB membrane proteins are postulated to form a pore for the transport of the DNA across the bacterial membranes. Immunofluorescence and immunoelectron microscopy were used to study the transport pore complex. Three likely c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kumar RB,Xie YH,Das A

    更新日期:2000-05-01 00:00:00

  • Regulation and differential expression of gdhA encoding NADP-specific glutamate dehydrogenase in Neisseria meningitidis clinical isolates.

    abstract::Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pagliarulo C,Salvatore P,De Vitis LR,Colicchio R,Monaco C,Tredici M,Talà A,Bardaro M,Lavitola A,Bruni CB,Alifano P

    更新日期:2004-03-01 00:00:00

  • Cell envelope components contributing to biofilm growth and survival of Pseudomonas putida in low-water-content habitats.

    abstract::Bacteria in terrestrial habitats frequently reside as biofilm communities on surfaces that are unsaturated, i.e. biofilms are covered in water films varying in thickness depending on the environmental conditions. Water availability in these habitats is influenced by the osmolarity of the water (solute stress) and by c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: van de Mortel M,Halverson LJ

    更新日期:2004-05-01 00:00:00

  • Cell-cell communication in filamentous cyanobacteria.

    abstract::Although cytoplasmic bridges between adjacent cells in the filaments of nitrogen-fixing cyanobacteria have been known for decades, the existence also of a continuous periplasm along the filaments raised the possibility that alternative modes of communication between cells could be utilized. The latter hypothesis was i...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Haselkorn R

    更新日期:2008-11-01 00:00:00

  • Determinants of the streptococcal surface glycoprotein GspB that facilitate export by the accessory Sec system.

    abstract::GspB is a large cell-surface glycoprotein expressed by Streptococcus gordonii M99 that mediates binding of this organism to human platelets. This adhesin is glycosylated in the cytoplasm, and is then transported to the cell surface via an accessory Sec system. To assess the structural features of GspB that are needed ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bensing BA,Takamatsu D,Sullam PM

    更新日期:2005-12-01 00:00:00

  • In vitro selection of miltefosine resistance in promastigotes of Leishmania donovani from Nepal: genomic and metabolomic characterization.

    abstract::In this study, we followed the genomic, lipidomic and metabolomic changes associated with the selection of miltefosine (MIL) resistance in two clinically derived Leishmania donovani strains with different inherent resistance to antimonial drugs (antimony sensitive strain Sb-S; and antimony resistant Sb-R). MIL-R was e...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Shaw CD,Lonchamp J,Downing T,Imamura H,Freeman TM,Cotton JA,Sanders M,Blackburn G,Dujardin JC,Rijal S,Khanal B,Illingworth CJ,Coombs GH,Carter KC

    更新日期:2016-03-01 00:00:00

  • Bacillus subtilis tetA(L) gene expression: evidence for regulation by translational reinitiation.

    abstract::The tetA(L) gene of Bacillus subtilis encodes a transmembrane protein that can function as a Tc-metal/H+ antiporter, conferring low-level resistance to tetracycline. The TetA(L) coding sequence is preceded by a leader region that contains a 20-amino-acid open reading frame and an appropriately spaced ribosome binding ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Stasinopoulos SJ,Farr GA,Bechhofer DH

    更新日期:1998-12-01 00:00:00

  • Entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG-3.

    abstract::HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demon...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Serrano JJ,de la Garza M,Moreno MA,Tovar R,León G,Tsutsumi V,Muñoz ML

    更新日期:1994-02-01 00:00:00

  • tRNAPro -mediated downregulation of elongation factor P is required for mgtCBR expression during Salmonella infection.

    abstract::Bacterial ribosome requires elongation factor P to translate fragments harbouring consecutive proline codons. Given the abundance of ORFs with potential EF-P regulated sites, EF-P was assumed to be constitutively expressed. Here, we report that the intracellular pathogen Salmonella enterica serovar Typhimurium decreas...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nam D,Choi E,Shin D,Lee EJ

    更新日期:2016-10-01 00:00:00

  • Characterization of the molecular mechanisms involved in the differential production of erythrose-4-phosphate dehydrogenase, 3-phosphoglycerate kinase and class II fructose-1,6-bisphosphate aldolase in Escherichia coli.

    abstract::A gapA-pgk gene tandem coding the glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase, is most frequently found in bacteria. However, in Enterobacteriaceae, gapA is replaced by an epd open reading frame (ORF) coding an erythrose-4-phosphate dehydrogenase and an fbaA ORF coding the class II fructose-...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bardey V,Vallet C,Robas N,Charpentier B,Thouvenot B,Mougin A,Hajnsdorf E,Régnier P,Springer M,Branlant C

    更新日期:2005-09-01 00:00:00

  • Probing bacterial cell biology using image cytometry.

    abstract::Advances in automated fluorescence microscopy have made snapshot and time-lapse imaging of bacterial cells commonplace, yet fundamental challenges remain in analysis. The vast quantity of data collected in high-throughput experiments requires a fast and reliable automated method to analyze fluorescence intensity and l...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cass JA,Stylianidou S,Kuwada NJ,Traxler B,Wiggins PA

    更新日期:2017-03-01 00:00:00

  • Sulfane Sulfur is an intrinsic signal activating MexR-regulated antibiotic resistance in Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa PAO1, an opportunistic human pathogen, deploys several strategies to resist antibiotics. It uses multidrug efflux pumps, including the MexAB-OprM pump, for antibiotic resistance, and it also produces hydrogen sulfide (H2 S) that provides some defense against antibiotics. MexR functions as a tran...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Xuan G,Lü C,Xu H,Chen Z,Li K,Liu H,Liu H,Xia Y,Xun L

    更新日期:2020-12-01 00:00:00

  • Mutational analysis of MarR, the negative regulator of marRAB expression in Escherichia coli, suggests the presence of two regions required for DNA binding.

    abstract::MarR, the negative regulator of the Escherichia coli multiple antibiotic resistance (marRAB) operon, is a member of a newly recognized family of regulatory proteins. The amino acid sequences of these proteins do not display any apparent homologies to the DNA binding domains of prokaryotic transcription regulators and ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Alekshun MN,Kim YS,Levy SB

    更新日期:2000-03-01 00:00:00

  • Biochemical analysis and structure determination of Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 muteins reveal the PHB binding site and details of substrate-enzyme interactions.

    abstract::Five amino acids (Y105, Y176, Y189, Y189, W207) that constitute the substrate binding site of PHB depolymerase PhaZ7 were identified. All residues are located at a single surface-exposed location of PhaZ7. Exchange of these amino acids by less hydrophobic, hydrophilic or negatively charged residues reduced binding of ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Jendrossek D,Hermawan S,Subedi B,Papageorgiou AC

    更新日期:2013-11-01 00:00:00

  • Sulphated tyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC).

    abstract::Plasmodium vivax is one of four Plasmodium species that cause human malaria. P. vivax and a related simian malaria parasite, Plasmodium knowlesi, invade erythrocytes by binding the Duffy antigen/receptor for chemokines (DARC) through their respective Duffy binding proteins. Here we show that tyrosines 30 and 41 of DAR...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Choe H,Moore MJ,Owens CM,Wright PL,Vasilieva N,Li W,Singh AP,Shakri R,Chitnis CE,Farzan M

    更新日期:2005-03-01 00:00:00

  • Organization of the genes necessary for hydrogenase expression in Rhodobacter capsulatus. Sequence analysis and identification of two hyp regulatory mutants.

    abstract::A 25 kbp DNA fragment from the chromosome of Rhodobacter capsulatus B10 carrying hydrogenase (hup) determinants was completely sequenced. Coding regions corresponding to 20 open reading frames were identified. The R. capsulatus hydrogenase-specific gene (hup and hyp) products bear significant structural identity to hy...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Colbeau A,Richaud P,Toussaint B,Caballero FJ,Elster C,Delphin C,Smith RL,Chabert J,Vignais PM

    更新日期:1993-04-01 00:00:00

  • Human pregnancy-associated malaria-specific B cells target polymorphic, conformational epitopes in VAR2CSA.

    abstract::Pregnancy-associated malaria (PAM) is caused by Plasmodium falciparum-infected erythrocytes (IEs) that bind to chondroitin sulphate A (CSA) in the placenta by PAM-associated clonally variant surface antigens (VSA). Pregnancy-specific VSA (VSA(PAM)), which include the PfEMP1 variant VAR2CSA, are targets of IgG-mediated...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Barfod L,Bernasconi NL,Dahlbäck M,Jarrossay D,Andersen PH,Salanti A,Ofori MF,Turner L,Resende M,Nielsen MA,Theander TG,Sallusto F,Lanzavecchia A,Hviid L

    更新日期:2007-01-01 00:00:00

  • Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.

    abstract::mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Moe PC,Blount P,Kung C

    更新日期:1998-05-01 00:00:00

  • Specific and pleiotropic patterns of mRNA regulation by ArcZ, a conserved, Hfq-dependent small RNA.

    abstract::The small RNA, ArcZ (previously RyhA/SraH), was discovered in several genome-wide screens in Escherichia coli and Salmonella. Its high degree of genomic conservation, its frequent recovery by shotgun sequencing, and its association with the RNA chaperone, Hfq, identified ArcZ as an abundant enterobacterial 'core' smal...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Papenfort K,Said N,Welsink T,Lucchini S,Hinton JCD,Vogel J

    更新日期:2009-10-01 00:00:00

  • The Sinorhizobium meliloti stringent response affects multiple aspects of symbiosis.

    abstract::Sinorhizobium meliloti and host legumes enter into a nitrogen-fixing, symbiotic relationship triggered by an exchange of signals between bacteria and plant. S. meliloti produces Nod factor, which elicits the formation of nodules on plant roots, and succinoglycan, an exopolysaccharide that allows for bacterial invasion...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wells DH,Long SR

    更新日期:2002-03-01 00:00:00

  • A novel DNA modification by sulphur.

    abstract::Streptomyces lividans has a novel DNA modification, which sensitises its DNA to degradation during electrophoresis (the Dnd phenotype). The entire gene cluster (dnd) involved in this modification was localized on an 8 kb DNA fragment and was expressed in a S. lividans deletion mutant (dnd) and in several heterologous ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zhou X,He X,Liang J,Li A,Xu T,Kieser T,Helmann JD,Deng Z

    更新日期:2005-09-01 00:00:00

  • Differential roles for the Co(2+) /Ni(2+) transporting ATPases, CtpD and CtpJ, in Mycobacterium tuberculosis virulence.

    abstract::The genome of Mycobacterium tuberculosis encodes two paralogous P1 B 4 -ATPases, CtpD (Rv1469) and CtpJ (Rv3743). Both proteins showed ATPase activation by Co(2+) and Ni(2+) , and both appear to be required for metal efflux from the cell. However, using a combination of biochemical and genetic studies we found that th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Raimunda D,Long JE,Padilla-Benavides T,Sassetti CM,Argüello JM

    更新日期:2014-01-01 00:00:00