Abstract:
:In Aspergillus nidulans, proline can serve both as a carbon and a nitrogen source. The transcription of the prnB gene, encoding the proline transporter, is efficiently repressed only by the simultaneous presence of ammonium and glucose. Thus, repression of this gene demands the activation of the CreA repressor and the inactivation of the positive-acting GATA factor AreA. Repression of all other prn structural genes results largely from inducer exclusion. In an areA null mutation background, prnB is repressible by the sole presence of glucose. We have determined by EMSA and missing-base interference experiments that there are 15 AreA-binding sites in the prnD-prnB intergenic region. Only sites 13/14, in the proximity of the prnB TATA box, are clearly involved in transcriptional activation and regulation. Mutation of these sites mimics qualitatively the regulatory effect of an areA null mutation. The deletion of the TATA box has a measurable effect on the maximal level of prnB transcription but does not alter the regulation pattern of this gene.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Gómez D,García I,Scazzocchio C,Cubero Bdoi
10.1046/j.1365-2958.2003.03682.xsubject
Has Abstractpub_date
2003-10-01 00:00:00pages
277-89issue
1eissn
0950-382Xissn
1365-2958pii
3682journal_volume
50pub_type
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