Abstract：
:The energy requirement for the second step in pullulanase secretion by the general secretory pathway was studied in Escherichia coli. In order to uncouple the two steps in the secretion pathway (across the cytoplasmic and outer membranes, respectively) and to facilitate kinetic analysis of secretion, a variant form of pullulanase lacking its N-terminal fatty acid membrane anchor was used. The transport of the periplasmic secretion intermediate form of this protein across the outer membrane was not inhibited by concentrations of sodium arsenate in excess of those required to reduce ATP levels to < or = 10% of their normal value. Pullulanase secretion was inhibited by the protonophore carbonyl cyanide m-chlorophenyl hydrazone at concentrations which were similar to those reported by others to be required to prevent solute uptake or the export and processing of preproteins across the cytoplasmic membrane, but which were in excess of those required to fully dissipate the proton-motive force and to reduce lactose uptake to a significant extent.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Possot OM,Letellier L,Pugsley APdoi
10.1046/j.1365-2958.1997.3451726.xsubject
Has Abstractpub_date
1997-05-01 00:00:00pages
457-64issue
3eissn
0950-382Xissn
1365-2958journal_volume
24pub_type
杂志文章abstract：:Previous work demonstrated that selection for Escherichia coli mutants with low antibiotic resistance frequently resulted in co-selection of lon mutations and that lon(-) mutants evolved higher-level resistance faster than a lon(+) strain. Here we show that lon mutation causes a very low multidrug resistance by induci...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12429
更新日期：2013-12-01 00:00:00
abstract：:Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03907.x
更新日期：2004-02-01 00:00:00
abstract：:MreB shares a common prokaryotic ancestor with actin and is present in almost all rod-shaped bacteria. MreB proteins have been implicated in a range of important cell processes, including cell morphogenesis, chromosome segregation and cell polarity. The mreB gene frequently lies at the beginning of a cluster of genes,...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04506.x
更新日期：2005-03-01 00:00:00
abstract：:Lipids are essential structural constituents of bacterial cell membranes and walls, and their biosynthetic pathways are stringently regulated at both biochemical and genetic levels. The recent surge of new information about transcriptional regulation of bacterial lipid metabolism is highlighted by two studies in this ...
journal_title：Molecular microbiology
pub_type： 评论,杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：:The phenomenon of cell-density-dependent control of gene expression, called autoinduction, has long been a subject of interest and investigation in bioluminescent marine bacteria. It is now becoming clear that many other bacteria, including animal and plant pathogens, use an autoinduction mechanism to regulate a varie...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1995.mmi_17050801.x
更新日期：1995-09-01 00:00:00
abstract：:A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchron...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.mmi_17020211.x
更新日期：1995-07-01 00:00:00
abstract：:Inhibitors of protein synthesis cause defects in the assembly of ribosomal subunits. In response to treatment with the antibiotics erythromycin or chloramphenicol, precursors of both large and small ribosomal subunits accumulate. We have used a pulse-labelling approach to demonstrate that the accumulating subribosomal...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2011.07555.x
更新日期：2011-04-01 00:00:00
abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05984.x
更新日期：2007-12-01 00:00:00
abstract：:The virulent phenotype of Shigella requires loci on the chromosome as well as on the large virulence plasmid, and is regulated via a complex web of interactions amongst various chromosomal and large plasmid genes. To further investigate the role of chromosomal loci in virulence, we performed random Tn10 mutagenesis in...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.405941.x
更新日期：1996-02-01 00:00:00
abstract：:A new locus required for type 4 pilus biogenesis by Pseudomonas aeruginosa has been identified. A pilE mutant, designated MJ-6, was broadly resistant to pili-specific phages and unable to translocate across solid surfaces by the pilus-dependent mechanism of twitching motility (Twt-). Immunoblot analysis demonstrated t...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb00489.x
更新日期：1994-09-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that is notorious for its intrinsic drug resistance. We have used chemical and genetic techniques to characterize three putative kinase genes that are involved in the addition of phosphate to the inner core region of P. aeruginosa lipopolysaccharide. The first gene i...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01741.x
更新日期：2000-02-01 00:00:00
abstract：:We have analysed the capacity of the 11 phase-variable, opacity-associated (Opa) proteins encoded by Neisseria gonorrhoeae MS11 to mediate traversal across polarized monolayers of the human colonic carcinoma T84 cell line. Gonococci expressing either the heparan sulphate proteoglycan (HSPG) binding Opa protein (Opa50)...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1998.01102.x
更新日期：1998-11-01 00:00:00
abstract：:The tra operon of the prototypical F plasmid and its relatives enables transfer of a copy of the plasmid to other bacterial cells via the process of conjugation. Tra proteins assemble to form the transferosome, the transmembrane pore through which the DNA is transferred, and the relaxosome, a complex of DNA-binding pr...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2012.08131.x
更新日期：2012-08-01 00:00:00
abstract：:One often compares cells to computers, and signalling proteins to transistors. Location and wiring of those molecular transistors is paramount in defining the function of the subcellular chips. The bacterial chemotactic sensing apparatus is a large, stable assembly consisting of thousands of receptors, signal transduc...
journal_title：Molecular microbiology
pub_type： 评论,杂志文章
doi：10.1111/j.1365-2958.2008.06240.x
更新日期：2008-07-01 00:00:00
abstract：:Engulfment in Bacillus subtilis is mediated by two complementary systems, SpoIID, SpoIIM and SpoIIP (DMP), which are essential for engulfment, and the SpoIIQ-SpoIIIAGH (Q-AH) zipper, which provides a secondary engulfment mechanism and recruits other proteins to the septum. We here identify two mechanisms by which DMP ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05887.x
更新日期：2007-09-01 00:00:00
abstract：:In this paper we show that the Escherichia coli protein Fis has a regulatory function in Mu transposition in the presence of Mu repressor. Fis can lower the transposition frequency of a mini-Mu 3-80-fold, but only if the Mu repressor is expressed simultaneously. In this novel type of regulation of transposition by the...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01955.x
更新日期：1993-10-01 00:00:00
abstract：:Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00157.x
更新日期：1989-12-01 00:00:00
abstract：:Helicobacter pylori persists deep in the human gastric mucus layer in a harsh, nutrient-poor environment. Survival under these conditions depends on the ability of this human pathogen to invoke starvation/stress responses when needed. Unlike many bacteria, H. pylori lacks starvation/stress-responding alternative sigma...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2010.07233.x
更新日期：2010-08-01 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae starvation for nitrogen on a glucose-containing medium causes entrance into G0 and downregulation of all targets of the PKA pathway. Re-addition of a nitrogen source in the presence of glucose causes rapid activation of trehalase and other PKA targets. Trehalase activation upon am...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.05043.x
更新日期：2006-03-01 00:00:00
abstract：:Expression of type 1 fimbriae in Salmonella enterica serovar Typhimurium undergoes phase variation or alteration between a fimbriate and a non-fimbriate phenotype. This variation is known to be dependent upon environmental conditions in vitro and is thought to be a complex process involving regulation by a number of p...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02430.x
更新日期：2001-05-01 00:00:00
abstract：:Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06253.x
更新日期：2008-06-01 00:00:00
abstract：:We have determined the nucleotide sequence changes caused by three missense mutations leading to the production of inactive colicin E3 proteins. The ceaC1 mutation, affecting the translocation of colicin E3 through bacterial membranes, is caused by a serine to phenylalanine change at position 37 within the glycine-ric...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1987.tb00530.x
更新日期：1987-07-01 00:00:00
abstract：:RelA and SpoT of Gram-negative organisms critically regulate cellular levels of (p)ppGpp. Here, we have dissected the spoT gene function of the cholera pathogen Vibrio cholerae by extensive genetic analysis. Unlike Escherichia coli, V. choleraeDeltarelADeltaspoT cells accumulated (p)ppGpp upon fatty acid or glucose st...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06653.x
更新日期：2009-04-01 00:00:00
abstract：:The bacterial flagellum contains a specialized secretion apparatus in its base that pumps certain protein subunits through the growing structure to their sites of installation beyond the membrane. A related apparatus functions in the injectisomes of gram-negative pathogens to export virulence factors into host cells. ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13623
更新日期：2017-04-01 00:00:00
abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13329
更新日期：2016-05-01 00:00:00
abstract：:Many physiological adjustments to nutrient changes involve ppGpp. Recent attempts to deduce ppGpp regulatory effects using proteomics or gene profiling can rigorously identify proteins or transcripts, but the functional significance is often unclear. Using a random screen for synthetic lethals we found a ppGpp-depende...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06317.x
更新日期：2008-08-01 00:00:00
abstract：:In eukaryotes, frataxin deficiency (FXN) causes severe phenotypes including loss of iron-sulfur (Fe-S) cluster protein activity, accumulation of mitochondrial iron and leads to the neurodegenerative disease Friedreich's ataxia. In contrast, in prokaryotes, deficiency in the FXN homolog, CyaY, was reported not to cause...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12888
更新日期：2015-02-01 00:00:00
abstract：:The nucleotide sequence of the traT gene present in the virulence-associated plasmid of Salmonella typhimurium was determined. The predicted TraT protein encoded by this gene was found to consist of 243 amino acids and to resemble the known TraT proteins of the plasmids of the F incompatibility group. Thus it contains...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb02014.x
更新日期：1990-01-01 00:00:00
abstract：:The differences between archaeal and bacterial flagella are becoming more apparent as research on the archaeal structure progresses. One crucial difference is the presence of a leader peptide on archaeal preflagellins, which is removed from the flagellin prior to its incorporation into the flagellar filament. The enzy...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03758.x
更新日期：2003-11-01 00:00:00
abstract：:The regulation of gene expression by the two-component regulatory system PhoP/PhoQ is necessary for Salmonella typhimurium survival within macrophages, defensin resistance, acid resistance, and murine typhoid fever pathogenesis. Salmonella experience multiple environments during mammalian infection and survival requir...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1991.tb02135.x
更新日期：1991-09-01 00:00:00
