Antibiotic-induced ribosomal assembly defects result from changes in the synthesis of ribosomal proteins.


:Inhibitors of protein synthesis cause defects in the assembly of ribosomal subunits. In response to treatment with the antibiotics erythromycin or chloramphenicol, precursors of both large and small ribosomal subunits accumulate. We have used a pulse-labelling approach to demonstrate that the accumulating subribosomal particles maturate into functional 70S ribosomes. The protein content of the precursor particles is heterogeneous and does not correspond with known assembly intermediates. Mass spectrometry indicates that production of ribosomal proteins in the presence of the antibiotics correlates with the amounts of the individual ribosomal proteins within the precursor particles. Thus, treatment of cells with chloramphenicol or erythromycin leads to an unbalanced synthesis of ribosomal proteins, providing the explanation for formation of assembly-defective particles. The operons for ribosomal proteins show a characteristic pattern of antibiotic inhibition where synthesis of the first proteins is inhibited weakly but gradually increases for the subsequent proteins in the operon. This phenomenon most likely reflects translational coupling and allows us to identify other putative coupled non-ribosomal operons in the Escherichia coli chromosome.


Mol Microbiol


Molecular microbiology


Siibak T,Peil L,Dönhöfer A,Tats A,Remm M,Wilson DN,Tenson T,Remme J




Has Abstract


2011-04-01 00:00:00












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    journal_title:Molecular microbiology

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    authors: Wullt B,Bergsten G,Connell H,Röllano P,Gebretsadik N,Hull R,Svanborg C

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  • Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation.

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    journal_title:Molecular microbiology

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    authors: Mukamolova GV,Murzin AG,Salina EG,Demina GR,Kell DB,Kaprelyants AS,Young M

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    authors: Qutyan M,Paliotti M,Castric P

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    authors: Petersen SK

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    authors: Ma D,Alberti M,Lynch C,Nikaido H,Hearst JE

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  • Liver-specific protein 2: a Plasmodium protein exported to the hepatocyte cytoplasm and required for merozoite formation.

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    authors: Orito Y,Ishino T,Iwanaga S,Kaneko I,Kato T,Menard R,Chinzei Y,Yuda M

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    authors: Zamudio JR,Mittra B,Campbell DA,Sturm NR

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    authors: Gloeckl S,Ong VA,Patel P,Tyndall JD,Timms P,Beagley KW,Allan JA,Armitage CW,Turnbull L,Whitchurch CB,Merdanovic M,Ehrmann M,Powers JC,Oleksyszyn J,Verdoes M,Bogyo M,Huston WM

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  • Analysis of the gene cluster involved in production and immunity of the peptide antibiotic AS-48 in Enterococcus faecalis.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

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  • Structure and function of DnaA and the DnaA-box in eubacteria: evolutionary relationships of bacterial replication origins.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Yoshikawa H,Ogasawara N

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Chessa D,Winter MG,Nuccio SP,Tükel C,Bäumler AJ

    更新日期:2008-05-01 00:00:00

  • Insertion of inverted Ter sites into the terminus region of the Escherichia coli chromosome delays completion of DNA replication and disrupts the cell cycle.

    abstract::To investigate the co-ordination between DNA replication and cell division, we have disrupted the DNA-replication cycle of Escherichia coli by inserting inverted Ter sites into the terminus region to delay completion of the chromosome. The inverted Ter sites (designated InvTer::spcr) were initially inserted into the c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sharma B,Hill TM

    更新日期:1995-10-01 00:00:00

  • Cloning and characterization of fxp, the flexible pilin gene of Aeromonas hydrophila.

    abstract::The flexible pilus of Aeromonas hydrophila is a morphologically and biochemically unique organelle which binds eukaryotic cell surfaces and whose expression is induced by specific physiochemical conditions. fxp, the structural gene coding for the flexible pilus subunit, was localized on a 7.6kb plasmid of A. hydrophil...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ho AS,Sohel I,Schoolnik GK

    更新日期:1992-09-01 00:00:00

  • Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)-D-mannose.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lightfoot J,Lam JS

    更新日期:1993-05-01 00:00:00

  • Identifying a novel connection between the fungal plasma membrane and pH-sensing.

    abstract::The mechanisms by which micro-organisms sense and internalize extracellular pH signals are not completely understood. One example of a known external pH-sensing process is the fungal-specific Rim/Pal signal transduction pathway. Fungi, such as the opportunistic pathogen Cryptococcus neoformans, use Rim signaling to se...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Brown HE,Ost KS,Esher SK,Pianalto KM,Saelens JW,Guan Z,Andrew Alspaugh J

    更新日期:2018-08-01 00:00:00

  • PhoP/PhoQ: macrophage-specific modulators of Salmonella virulence?

    abstract::The regulation of gene expression by the two-component regulatory system PhoP/PhoQ is necessary for Salmonella typhimurium survival within macrophages, defensin resistance, acid resistance, and murine typhoid fever pathogenesis. Salmonella experience multiple environments during mammalian infection and survival requir...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Miller SI

    更新日期:1991-09-01 00:00:00

  • Novel carbohydrate binding modules in the surface anchored α-amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cockburn DW,Suh C,Medina KP,Duvall RM,Wawrzak Z,Henrissat B,Koropatkin NM

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Chitlaru T,Zaide G,Ehrlich S,Inbar I,Cohen O,Shafferman A

    更新日期:2011-09-01 00:00:00