Antibiotic-induced ribosomal assembly defects result from changes in the synthesis of ribosomal proteins.


:Inhibitors of protein synthesis cause defects in the assembly of ribosomal subunits. In response to treatment with the antibiotics erythromycin or chloramphenicol, precursors of both large and small ribosomal subunits accumulate. We have used a pulse-labelling approach to demonstrate that the accumulating subribosomal particles maturate into functional 70S ribosomes. The protein content of the precursor particles is heterogeneous and does not correspond with known assembly intermediates. Mass spectrometry indicates that production of ribosomal proteins in the presence of the antibiotics correlates with the amounts of the individual ribosomal proteins within the precursor particles. Thus, treatment of cells with chloramphenicol or erythromycin leads to an unbalanced synthesis of ribosomal proteins, providing the explanation for formation of assembly-defective particles. The operons for ribosomal proteins show a characteristic pattern of antibiotic inhibition where synthesis of the first proteins is inhibited weakly but gradually increases for the subsequent proteins in the operon. This phenomenon most likely reflects translational coupling and allows us to identify other putative coupled non-ribosomal operons in the Escherichia coli chromosome.


Mol Microbiol


Molecular microbiology


Siibak T,Peil L,Dönhöfer A,Tats A,Remm M,Wilson DN,Tenson T,Remme J




Has Abstract


2011-04-01 00:00:00












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    abstract::The availability of nutrients is a major determinant for the timing of morphogenesis and antibiotic production in the soil-dwelling bacterium Streptomyces coelicolor. Here we show that N-acetylglucosamine transport, the first step of an important nutrient signalling cascade, is mediated by the NagE2 permease of the ph...

    journal_title:Molecular microbiology

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    authors: Nothaft H,Rigali S,Boomsma B,Swiatek M,McDowall KJ,van Wezel GP,Titgemeyer F

    更新日期:2010-03-01 00:00:00

  • A novel mechanism of phase variation of virulence in Staphylococcus epidermidis: evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256.

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    journal_title:Molecular microbiology

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    authors: Ziebuhr W,Krimmer V,Rachid S,Lössner I,Götz F,Hacker J

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  • Antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in Escherichia coli.

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    journal_title:Molecular microbiology

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    authors: Wahl A,My L,Dumoulin R,Sturgis JN,Bouveret E

    更新日期:2011-06-01 00:00:00

  • Post-transcriptional regulation by distal Shine-Dalgarno sequences in the grpE-dnaK intergenic region of Streptococcus mutans.

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    journal_title:Molecular microbiology

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    authors: Palmer SR,Burne RA

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    journal_title:Molecular microbiology

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    authors: Kawano M,Aravind L,Storz G

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    journal_title:Molecular microbiology

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    authors: Papenfort K,Pfeiffer V,Lucchini S,Sonawane A,Hinton JC,Vogel J

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    authors: Yang H,Lipscomb GL,Keese AM,Schut GJ,Thomm M,Adams MW,Wang BC,Scott RA

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    pub_type: 杂志文章,评审


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    authors: Neale HC,Jackson RW,Preston GM,Arnold DL

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    pub_type: 杂志文章


    authors: Nodwell JR,McGovern K,Losick R

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    authors: Joly N,Rappas M,Wigneshweraraj SR,Zhang X,Buck M

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  • Endoplasmic reticulum localized PerA is required for cell wall integrity, azole drug resistance, and virulence in Aspergillus fumigatus.

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    authors: Chung D,Thammahong A,Shepardson KM,Blosser SJ,Cramer RA

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    authors: Potluri L,Karczmarek A,Verheul J,Piette A,Wilkin JM,Werth N,Banzhaf M,Vollmer W,Young KD,Nguyen-Distèche M,den Blaauwen T

    更新日期:2010-07-01 00:00:00

  • Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation.

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    pub_type: 杂志文章


    authors: Baynham PJ,Brown AL,Hall LL,Wozniak DJ

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  • RecA-mediated excision repair: a novel mechanism for repairing DNA lesions at sites of arrested DNA synthesis.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bichara M,Pinet I,Lambert IB,Fuchs RP

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  • Characterization of the pathway for transport of the cytoadherence-mediating protein, PfEMP1, to the host cell surface in malaria parasite-infected erythrocytes.

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    pub_type: 杂志文章


    authors: Kriek N,Tilley L,Horrocks P,Pinches R,Elford BC,Ferguson DJ,Lingelbach K,Newbold CI

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    pub_type: 杂志文章,评审


    authors: Praetorius-Ibba M,Ibba M

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    authors: Ding M,Kwok LY,Schlüter D,Clayton C,Soldati D

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  • Leishmania mexicana can utilize amino acids as major carbon sources in macrophages but not in animal models.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Saunders EC,Naderer T,Chambers J,Landfear SM,McConville MJ

    更新日期:2018-04-01 00:00:00

  • The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins.

    abstract::The chromosomes of the soil bacteria Streptomyces, unlike those of most other bacteria, are linear DNA molecules. Their telomeres contain long-terminal inverted repeats and covalently bound terminal proteins (TPs). These bacteria also harbour linear plasmids that share the same structural features. In this study, we d...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Yang CC,Huang CH,Li CY,Tsay YG,Lee SC,Chen CW

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  • The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Svensson SL,Davis LM,MacKichan JK,Allan BJ,Pajaniappan M,Thompson SA,Gaynor EC

    更新日期:2009-01-01 00:00:00

  • Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants.

    abstract::Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Andrews KT,Pirrit LA,Przyborski JM,Sanchez CP,Sterkers Y,Ricken S,Wickert H,Lépolard C,Avril M,Scherf A,Gysin J,Lanzer M

    更新日期:2003-08-01 00:00:00

  • Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility.

    abstract::Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Asakura H,Churin Y,Bauer B,Boettcher JP,Bartfeld S,Hashii N,Kawasaki N,Mollenkopf HJ,Jungblut PR,Brinkmann V,Meyer TF

    更新日期:2010-12-01 00:00:00

  • A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides.

    abstract::The AppA protein of Rhodobacter sphaeroides is unique in its ability to sense and transmit redox signals as well as light signals. By functioning as antagonist to the PpsR transcriptional repressor, it regulates the expression of photosynthesis genes in response to these environmental stimuli. Here we show binding of ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Han Y,Meyer MH,Keusgen M,Klug G

    更新日期:2007-05-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sohel I,Puente JL,Murray WJ,Vuopio-Varkila J,Schoolnik GK

    更新日期:1993-02-01 00:00:00

  • The porA gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation.

    abstract::Molecular analyses were applied to the genes encoding variants of the serosubtyping antigen, the class 1 outer membrane protein (PorA), from 55 serogroup A Neisseria meningitidis strains. These genes were evolutionarily stable and exhibited a limited range of genetic variation, primarily generated by recombination. Tr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Suker J,Feavers IM,Achtman M,Morelli G,Wang JF,Maiden MC

    更新日期:1994-04-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Meehan BM,Landeta C,Boyd D,Beckwith J

    更新日期:2017-02-01 00:00:00