Abstract:
:Three distinct types of terminal oxidases participate in the aerobic respiratory pathways of Paracoccus denitrificans. Two alternative genes encoding subunit I of the aa3-type cytochrome c oxidase have been isolated before, namely ctaDI and ctaDII. Each of these genes can be expressed separately to complement a double mutant (delta ctaDI, delta ctaDII), indicating that they are isoforms of subunit I of the aa3-type oxidase. The genomic locus of a quinol oxidase has been isolated: cyoABC. This protohaem-containing oxidase, called cytochrome bb3, is the only quinol oxidase expressed under the conditions used. In a triple oxidase mutant (delta ctaDI, delta ctaDII, cyoB::KmR) an alternative cytochrome c oxidase has been characterized; this cbb3-type oxidase has been partially purified. Both cytochrome aa3 and cytochrome bb3 are redox-driven proton pumps. The proton-pumping capacity of cytochrome cbb3 has been analysed; arguments for and against the active transport of protons by this novel oxidase complex are discussed.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
de Gier JW,Lübben M,Reijnders WN,Tipker CA,Slotboom DJ,van Spanning RJ,Stouthamer AH,van der Oost Jdoi
10.1111/j.1365-2958.1994.tb00414.xsubject
Has Abstractpub_date
1994-07-01 00:00:00pages
183-96issue
2eissn
0950-382Xissn
1365-2958journal_volume
13pub_type
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