当前位置： SCI文献检索 > MOLECULAR MICROBIOLOGY期刊下所有文献 > Signalling substitutions in the periplasmic domain of chemoreceptor Trg induce or reduce helical sliding in the transmembrane domain.

Signalling substitutions in the periplasmic domain of chemoreceptor Trg induce or reduce helical sliding in the transmembrane domain.

Abstract：

:We used in vivo oxidative cross-linking of engineered cysteine pairs to assess conformational changes in the four-helix transmembrane domain of chemoreceptor Trg. Extending previous work, we searched for and found a fourth cross-linking pair that spanned the intrasubunit interface between transmembrane helix 1 (TM1) and its partner TM2. We determined the effects of ligand occupancy on cross-linking rate constants for all four TM1-TM2 diagnostic pairs in conditions that allowed the formation of receptor-kinase complexes for the entire cellular complement of Trg. Occupancy altered all four rates in a pattern that implicated sliding of TM2 relative to TM1 towards the cytoplasm as the transmembrane signalling movement in receptor-kinase complexes. Transmembrane signalling can be reduced or induced by single amino acid substitutions in the ligand-binding region of the periplasmic domain of Trg. We determined the effects of these substitutions on conformation in the transmembrane domain and on ligand-induced changes using the diagnostic TM1-TM2 cysteine pairs. Effects on rates of in vivo cross-linking showed that induced signalling substitutions altered the relative positions of TM1 and TM2 in the same way as ligand binding, and reduced signalling substitutions blocked or attenuated the ligand-induced shift. These results provide strong support for the helical sliding model of transmembrane signalling.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Beel BD,Hazelbauer GL

doi

10.1046/j.1365-2958.2001.02446.x

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

824-34

issue

4

eissn

0950-382X

issn

1365-2958

pii

mmi2446

journal_volume

40

pub_type

杂志文章

相关文献

MOLECULAR MICROBIOLOGY文献大全
  • Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ.

    abstract：:Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2012.08025.x

    authors： Thomas JA,Weintraub ST,Wu W,Winkler DC,Cheng N,Steven AC,Black LW

    更新日期：2012-04-01 00:00:00

  • A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor.

    abstract：:Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2005.04609.x

    authors： Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

    更新日期：2005-06-01 00:00:00

  • An increase in expression of a Mycobacterium tuberculosis mycolyl transferase gene (fbpB) occurs early after infection of human monocytes.

    abstract：:Changes in the mRNA levels of two Mycobacterium tuberculosis genes (fbpB known as antigen 85B, and hspX known as Acr) were studied in infected human monocytes. Antigen 85B is an enzyme involved in cell wall biosynthesis and is also a major target of the immune response. Acr is a stress protein believed to be involved ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2001.02280.x

    authors： Wilkinson RJ,DesJardin LE,Islam N,Gibson BM,Kanost RA,Wilkinson KA,Poelman D,Eisenach KD,Toossi Z

    更新日期：2001-02-01 00:00:00

  • Initial events in the degradation of the polycistronic puf mRNA in Rhodobacter capsulatus and consequences for further processing steps.

    abstract：:Individual segments of the polycistronic puf mRNA of Rhodobacter capsulatus exhibit extremely different half-lives contributing to the stoichiometry of light-harvesting and reaction centre complexes of this facultative phototrophic bacterium. While earlier investigations shed light on the processes leading to the degr...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.01679.x

    authors： Heck C,Balzer A,Fuhrmann O,Klug G

    更新日期：2000-01-01 00:00:00

  • Cloning and characterization of three genes (SUT1-3) encoding glucose transporters of the yeast Pichia stipitis.

    abstract：:We have identified and characterized three genes, SUT1, SUT2 and SUT3, that encode glucose transporters of the yeast Pichia stipitis. When expressed in a Saccharomyces cerevisiae hxt null mutant strain that is unable to take up monosaccharides, all three proteins restored growth on glucose. Sequencing of the genes rev...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1999.01224.x

    authors： Weierstall T,Hollenberg CP,Boles E

    更新日期：1999-02-01 00:00:00

  • Agrobacterium type IV secretion is a two-step process in which export substrates associate with the virulence protein VirJ in the periplasm.

    abstract：:Type IV secretion systems are virulence determinants in many bacteria and share extensive homology with many conjugal transfer systems. Although type IV systems and their homologues have been studied widely, the mechanism by which substrates are secreted remains unclear. In Agrobacterium, we show that type IV secretio...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2002.03098.x

    authors： Pantoja M,Chen L,Chen Y,Nester EW

    更新日期：2002-09-01 00:00:00

  • Structure of the type III secretion and substrate-binding domain of Yersinia YopH phosphatase.

    abstract：:Pathogenic strains of Yersinia deploy a type III secretion system to inject the potent tyrosine phosphatase YopH into host cells, where it dephosphorylates focal adhesion-associated substrates. The amino-terminal, non-catalytic domain of YopH is bifunctional; it is essential for the secretion and binding of the specif...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.0950-382x.2001.02711.x

    authors： Smith CL,Khandelwal P,Keliikuli K,Zuiderweg ER,Saper MA

    更新日期：2001-11-01 00:00:00

  • Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.

    abstract：:Coli surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.12044

    authors： Roy SP,Rahman MM,Yu XD,Tuittila M,Knight SD,Zavialov AV

    更新日期：2012-12-01 00:00:00

  • Location and orientation of an activating region in the Escherichia coli transcription factor, FNR.

    abstract：:We have characterized a number of mutations in fnr that interfere with FNR-dependent transcription activation at two promoters where the FNR-binding site is centred around 41 1/2 bp upstream from the transcription start site. The substituted residues in all but one of these FNR mutants are clustered around a presumed ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1994.tb00318.x

    authors： Bell A,Busby S

    更新日期：1994-01-01 00:00:00

  • Cellulosomes: microbial nanomachines that display plasticity in quaternary structure.

    abstract：:The assembly of proteins that display complementary activities into supramolecular intra- and extracellular complexes is central to cellular function. One such nanomachine of considerable biological and industrial significance is the plant cell wall degrading apparatus of anaerobic bacteria termed the cellulosome. The...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.2007.05640.x

    authors： Gilbert HJ

    更新日期：2007-03-01 00:00:00

  • Many of the genes required for mating in Saccharomyces cerevisiae are also required for mating in Candida albicans.

    abstract：:Candida albicans is the single, most frequently isolated human fungal pathogen. As with most fungal pathogens, the factors which contribute to pathogenesis in C. albicans are not known, despite more than a decade of molecular genetic analysis. Candida albicans was thought to be asexual until the discovery of the MTL l...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2002.03263.x

    authors： Magee BB,Legrand M,Alarco AM,Raymond M,Magee PT

    更新日期：2002-12-01 00:00:00

  • The Haemophilus influenzae Hia autotransporter harbours two adhesive pockets that reside in the passenger domain and recognize the same host cell receptor.

    abstract：:Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2002.03189.x

    authors： Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

    更新日期：2002-11-01 00:00:00

  • A recombinational defect in the C-terminal domain of Escherichia coli RecA2278-5 protein is compensated by protein binding to ATP.

    abstract：:RecA2278-5 is a mutant RecA protein (RecAmut) bearing two amino acid substitutions, Gly-278 to Thr and Val-275 to Phe, in the alpha-helix H of the C-terminal subdomain of the protein. RecA2278-5 mutant cells are unusual in that they are thermosensitive for recombination but almost normal for DNA repair of UV damage an...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1997.1961557.x

    authors： Alexseyev AA,Baitin DM,Kuramitsu S,Ogawa T,Ogawa H,Lanzov VA

    更新日期：1997-01-01 00:00:00

  • Ubiquitin-Yop hybrids as probes for post-translational transport by the Yersinia type III secretion pathway.

    abstract：:Yersinia enterocolitica uses type III secretion to transport Yop proteins into the cytoplasm of host cells. Previous work generated hypotheses for both co- and post-translational transport mechanisms in the Yersinia type III pathway. Here, we used ubiquitin (Ub) and UBP1, the Ub-specific protease, to examine whether Y...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2007.05793.x

    authors： Quenee LE,Schneewind O

    更新日期：2007-07-01 00:00:00

  • Structure-function analysis of the LytM domain of EnvC, an activator of cell wall remodelling at the Escherichia coli division site.

    abstract：:Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.12304

    authors： Peters NT,Morlot C,Yang DC,Uehara T,Vernet T,Bernhardt TG

    更新日期：2013-08-01 00:00:00

  • SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm.

    abstract：:SecA is the dissociable ATPase subunit of the Escherichia coli preprotein translocase, and cycles in a nucleotide-modulated manner between the cytosol and the membrane. Overproduction of the integral subunits of the translocase, the SecY, SecE and SecG polypeptides, results in an increased level of membrane-bound SecA...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1996.d01-1712.x

    authors： van der Does C,den Blaauwen T,de Wit JG,Manting EH,Groot NA,Fekkes P,Driessen AJ

    更新日期：1996-11-01 00:00:00

  • Dockerin-containing protease inhibitor protects key cellulosomal cellulases from proteolysis in Clostridium cellulolyticum.

    abstract：:Cellulosomes are key for lignocellulosic biomass degradation in cellulolytic Clostridia. Better understanding of the mechanism of cellulosome regulation would allow us to improve lignocellulose hydrolysis. It is hypothesized that cellulosomal protease inhibitors would regulate cellulosome architecture and then lignoce...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.12488

    authors： Xu T,Li Y,He Z,Zhou J

    更新日期：2014-02-01 00:00:00

  • The DNA site utilized by bacteriophage P22 for initiation of DNA packaging.

    abstract：:Virion proteins recognize their cognate nucleic acid for encapsidation into virions through recognition of a specific nucleotide sequence contained within that nucleic acid. Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive series ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2002.03114.x

    authors： Wu H,Sampson L,Parr R,Casjens S

    更新日期：2002-09-01 00:00:00

  • Coupling of Escherichia coli hns mRNA levels to DNA synthesis by autoregulation: implications for growth phase control.

    abstract：:The H-NS protein of enteric bacteria is one of the major proteins of the bacterial nucleoid and seems to play an important role in nucleoid structure. Transcription of the hns gene encoding the H-NS protein appears to be negatively regulated by H-NS itself both in vitro and in vivo. We have examined the role of this m...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1995.mmi_18010101.x

    authors： Free A,Dorman CJ

    更新日期：1995-10-01 00:00:00

  • Role of Escherichia coli DNA polymerase I in chromosomal DNA replication fidelity.

    abstract：:We have investigated the possible role of Escherichia coli DNA polymerase (Pol) I in chromosomal replication fidelity. This was done by substituting the chromosomal polA gene by the polAexo variant containing an inactivated 3'-->5' exonuclease, which serves as a proofreader for this enzyme's misinsertion errors. Using...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2009.06921.x

    authors： Makiela-Dzbenska K,Jaszczur M,Banach-Orlowska M,Jonczyk P,Schaaper RM,Fijalkowska IJ

    更新日期：2009-12-01 00:00:00

  • Characterization of the 2-ketogluconate utilization operon in Pseudomonas aeruginosa PAO1.

    abstract：:The Pseudomonas aeruginosa protein PtxS negatively regulates its own synthesis by binding to the upstream region of its gene. We have recently identified a 14 bp palindromic sequence within the ptxS upstream region as the PtxS operator site (OP1). In this study, we searched the P. aeruginosa genomic sequence to determ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.02012.x

    authors： Swanson BL,Hager P,Phibbs P Jr,Ochsner U,Vasil ML,Hamood AN

    更新日期：2000-08-01 00:00:00

  • The global regulatory protein FruR modulates the direction of carbon flow in Escherichia coli.

    abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1995.tb02339.x

    authors： Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jr

    更新日期：1995-06-01 00:00:00

  • Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.

    abstract：:Dormant bacterial spores are extraordinarily resistant to environmental insults and are vectors of various illnesses. However, spores cannot cause disease unless they germinate and become vegetative cells. The molecular details of initiation of germination are not understood, but proteins essential in early stages of ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2011.07753.x

    authors： Griffiths KK,Zhang J,Cowan AE,Yu J,Setlow P

    更新日期：2011-08-01 00:00:00

  • Multiple hok genes on the chromosome of Escherichia coli.

    abstract：:The hok/sok locus of plasmid R1 mediates plasmid stabilization by the killing of plasmid-free cells. Many bacterial plasmids carry similar loci. For example, the F plasmid carries two hok homologues, flm and srnB, that mediate plasmid stabilization by this specialized type of programmed cell death. Here, we show that ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1999.01431.x

    authors： Pedersen K,Gerdes K

    更新日期：1999-06-01 00:00:00

  • Localisation and interactions of the Vipp1 protein in cyanobacteria.

    abstract：:The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green f...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.12826

    authors： Bryan SJ,Burroughs NJ,Shevela D,Yu J,Rupprecht E,Liu LN,Mastroianni G,Xue Q,Llorente-Garcia I,Leake MC,Eichacker LA,Schneider D,Nixon PJ,Mullineaux CW

    更新日期：2014-10-13 00:00:00

  • The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice.

    abstract：:The gene encoding a Lon protease homologue has been cloned from Brucella abortus. The putative Brucella abortus Lon shares > 60% amino acid identity with its Escherichia coli counterpart and the recombinant form of this protein restores the capacity of an Escherichia coli lon mutant to resist killing by ultraviolet ir...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.01726.x

    authors： Robertson GT,Kovach ME,Allen CA,Ficht TA,Roop RM 2nd

    更新日期：2000-02-01 00:00:00

  • Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.

    abstract：:The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine th...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.2010.07455.x

    authors： Parveen N,Cornell KA

    更新日期：2011-01-01 00:00:00

  • The chemosensory systems of Vibrio cholerae.

    abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/mmi.14520

    authors： Ortega DR,Kjaer A,Briegel A

    更新日期：2020-09-01 00:00:00

  • Regulated transcription of Clostridium difficile toxin genes.

    abstract：:The Clostridium difficile toxA and toxB genes, encoding cytotoxic and enterotoxic proteins responsible for antibiotic-associated colitis and pseudomembranous colitis, were shown to be transcribed both from gene-specific promoters and from promoters of upstream genes. However, the gene-specific transcripts represented ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1998.00663.x

    authors： Dupuy B,Sonenshein AL

    更新日期：1998-01-01 00:00:00

  • Individual functions of the HAK and TRK potassium transporters of Schwanniomyces occidentalis.

    abstract：:We have cloned the gene encoding the TRK transporter of the soil yeast Schwanniomyces occidentalis and obtained the HAK1 trk1 delta and the hak1 delta TRK1 mutant strains. Analyses of the transport capacities of these mutants have shown that (i) the HAK1 and the TRK1 potassium transporters are the only transporters op...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.02040.x

    authors： Bañuelos MA,Madrid R,Rodríguez-Navarro A

    更新日期：2000-08-01 00:00:00