Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.

abstract：:We have examined the role of the F-plasmid TraV outer membrane lipoprotein in the assembly of F-pili. Yeast two-hybrid analysis with a traV bait repeatedly identified traK, which is predicted to encode a periplasmic protein, among positive prey plasmids. A traK bait in turn identified traV and traB, which is predicted...

journal_title：Molecular microbiology

authors： Harris RL,Hombs V,Silverman PM

更新日期：2001-11-01 00:00:00

abstract：:The lipopolysaccharide (LPS) of H. influenzae is highly variable. Much of the structural diversity is derived from phase variation, or high frequency on-off switching, of molecules attached during LPS biosynthesis. In this study, we examined the dynamics of LPS phase variation following exposure to human serum as a so...

journal_title：Molecular microbiology

authors： Clark SE,Eichelberger KR,Weiser JN

更新日期：2013-05-01 00:00:00

abstract：:The most abundant oxidants controlling bacterial colonization on mucosal barrier epithelia are hypochlorous acid (HOCl), hypobromous acid (HOBr) and hypothiocyanous acid (HOSCN). All three oxidants are highly antimicrobial but little is known about their relative efficacies, their respective cellular targets, or what ...

journal_title：Molecular microbiology

authors： Groitl B,Dahl JU,Schroeder JW,Jakob U

更新日期：2017-11-01 00:00:00

abstract：:The Salmonella enterica serotype Typhimurium (S. typhimurium) genome contains a large repertoire of putative fimbrial operons that remain poorly characterized because they are not expressed in vitro. In this study, insertions that induced expression of the putative stdABCD fimbrial operon were identified from a random...

journal_title：Molecular microbiology

authors： Chessa D,Winter MG,Nuccio SP,Tükel C,Bäumler AJ

更新日期：2008-05-01 00:00:00

abstract：:A region of 7.8 kb of the plasmid pMB2 from Enterococcus faecalis S-48 carrying the information necessary for production and immunity of the peptide antibiotic AS-48 has been cloned and sequenced. It contains the as-48A structural gene plus five open reading frames (as-48B, as-48C, as-48C1, as-48D and as-48D1). Beside...

journal_title：Molecular microbiology

authors： Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

更新日期：1998-01-01 00:00:00

abstract：:Programmed frameshifting in the RF2 gene (prfB) involves an intragenic Shine-Dalgarno (SD) sequence. To investigate the role of SD-ASD pairing in the mechanism of frameshifting, we have analysed the effect of spacing between the SD sequence and P codon on P-site tRNA binding and RF2-dependent termination. When the spa...

journal_title：Molecular microbiology

authors： Devaraj A,Fredrick K

更新日期：2010-12-01 00:00:00

abstract：:The Escherichia coli marRAB operon specifies two regulatory proteins, MarR (which represses) and MarA (which activates expression of the operon). The latter controls expression of multiple other chromosomal genes implicated in cell physiology, multiple drug resistance and virulence. Using randomly cloned E. coli DNA f...

journal_title：Molecular microbiology

authors： Domain F,Bina XR,Levy SB

更新日期：2007-10-01 00:00:00

abstract：:Sphingomyelin is the main sphingolipid in Trypanosoma brucei, the causative agent of African sleeping sickness. In vitro and in vivo characterization of the T. brucei neutral sphingomyelinase demonstrates that it is directly involved in sphingomyelin catabolism. Gene knockout studies in the bloodstream form of the par...

journal_title：Molecular microbiology

authors： Young SA,Smith TK

更新日期：2010-06-01 00:00:00

abstract：:When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtil...

journal_title：Molecular microbiology

authors： Wiegert T,Homuth G,Versteeg S,Schumann W

更新日期：2001-07-01 00:00:00

abstract：:The cytokinetic apparatus of bacteria is initially formed by the polymerization of the tubulin-like FtsZ protein into a ring structure at midcell. This so-called Z-ring facilitates the recruitment of many additional proteins to the division site to form the mature divisome machine. Although the assembly pathway leadin...

journal_title：Molecular microbiology

authors： Tsang MJ,Bernhardt TG

更新日期：2015-03-01 00:00:00

abstract：:In addition to being the major citric acid cycle aconitase in Escherichia coli the aconitase B protein (AcnB) is also a post-transcriptional regulator of gene expression. The AcnB proteins represent a distinct branch of the aconitase superfamily that possess a HEAT-like domain (domain 5). The HEAT domains of other pro...

journal_title：Molecular microbiology

authors： Tang Y,Guest JR,Artymiuk PJ,Green J

更新日期：2005-06-01 00:00:00

abstract：:Type IV secretion systems are virulence determinants in many bacteria and share extensive homology with many conjugal transfer systems. Although type IV systems and their homologues have been studied widely, the mechanism by which substrates are secreted remains unclear. In Agrobacterium, we show that type IV secretio...

journal_title：Molecular microbiology

authors： Pantoja M,Chen L,Chen Y,Nester EW

更新日期：2002-09-01 00:00:00

abstract：:Kinetoplastid topoisomerase IB is an unusual bisubunit enzyme where reconstitution of the large (LdTOPIL or L) and small (LdTOPIS or S) subunits shows functional activity. It is yet to be deciphered whether one subunit or both navigate the heterodimer to its cellular DNA targets. Tethering a specific DNA-binding prote...

journal_title：Molecular microbiology

authors： BoseDasgupta S,Ganguly A,Das BB,Roy A,Khalkho NV,Majumder HK

更新日期：2008-01-01 00:00:00

abstract：:Bacteriophage PRD1 is a tailless membrane-containing double-stranded (ds) DNA virus infecting a variety of Gram-negative bacteria. In order to affect cell lysis, like most dsDNA phages, PRD1 uses the holin-endolysin system. In this study, we identified two accessory lysis genes, XXXVI and XXXVII, coding for proteins P...

journal_title：Molecular microbiology

authors： Krupovic M,Cvirkaite-Krupovic V,Bamford DH

更新日期：2008-04-01 00:00:00

abstract：:Crs5 is a Saccharomyces cerevisiae Metallothionein (MT), non-homologous to the paradigmatic Cu-thionein Cup1. Although considered a secondary copper-resistance agent, we show here that it determines survival under zinc overload in a CUP1-null background. Its overexpression prevents the deleterious effects exhibited by...

journal_title：Molecular microbiology

authors： Pagani A,Villarreal L,Capdevila M,Atrian S

更新日期：2007-01-01 00:00:00

abstract：:Protein kinase C constitutes a family of serine-threonine kinases found in all eukaryotes and implicated in a wide range of cellular functions, including regulation of cell growth, cellular differentiation and immunity. Here, we present three independent lines of evidence which indicate that protein kinase C is essent...

journal_title：Molecular microbiology

authors： Penn TJ,Wood ME,Soanes DM,Csukai M,Corran AJ,Talbot NJ

更新日期：2015-10-01 00:00:00

abstract：:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open readin...

journal_title：Molecular microbiology

authors： Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela C

更新日期：1991-11-01 00:00:00

abstract：:In response to nitrate availability, Escherichia coli regulates the synthesis of a number of enzymes involved in anaerobic respiration and fermentation. When nitrate is present, nitrate reductase (narGHJI) gene expression is induced, while expression of the DMSO/TMAO reductase (dmsABC), fumarate reductase (frdABCD) an...

journal_title：Molecular microbiology

authors： Chiang RC,Cavicchioli R,Gunsalus RP

更新日期：1992-07-01 00:00:00

abstract：:The de novo purine biosynthetic enzymes 5-amino-4-imidazolecarboxamide-ribonucleotide (AICAR) transformylase (EC 2.1.2.3), IMP cyclohydrolase (EC 3.5.4.10) and glycineamide-ribonucleotide (GAR) synthetase (EC 2.1.2.2) are encoded by the purHD locus of Escherichia coli. The DNA sequence of this locus revealed two open ...

journal_title：Molecular microbiology

authors： Flannigan KA,Hennigan SH,Vogelbacker HH,Gots JS,Smith JM

更新日期：1990-03-01 00:00:00

abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...

journal_title：Molecular microbiology

authors： Yang Y,Wang T,Yu Q,Liu H,Xun L,Xia Y

更新日期：2020-12-29 00:00:00

abstract：:A mechanistic hypothesis for the origin of the three domains of life is proposed. A population of evolving pre-cells is suggested to have had a membrane of a racemate of chiral lipids that continuously underwent spontaneous symmetry breaking by spatial phase segregation into two enantiomerically enriched membrane doma...

journal_title：Molecular microbiology

authors： Wächtershäuser G

更新日期：2003-01-01 00:00:00

abstract：:The Escherichia coli DNA glycosylase Mug excises 3,N(4)-ethenocytosines (epsilon C) and uracils from DNA, but its biological function is obscure. This is because epsilon C is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We ...

journal_title：Molecular microbiology

authors： Mokkapati SK,Fernández de Henestrosa AR,Bhagwat AS

更新日期：2001-09-01 00:00:00

abstract：:Avermectins produced by Streptomyces avermitilis are effective anthelmintic agents. The autoregulatory signalling molecule that triggers avermectin biosynthesis is a novel butenolide-type molecule, avenolide, rather than common γ-butyrolactones (GBLs). We identified AvaR2, a pseudo GBL receptor homologue, as an import...

journal_title：Molecular microbiology

authors： Zhu J,Sun D,Liu W,Chen Z,Li J,Wen Y

更新日期：2016-11-01 00:00:00

abstract：:We previously characterized a defective-folding mutant of maltose-binding protein of Escherichia coli, MalE31, which formed periplasmic inclusion bodies. Here, we show that MalE31 aggregation does not affect bacterial growth at 30 degrees C but is lethal at 37 degrees C. Surprisingly, under mild heat shock conditions ...

journal_title：Molecular microbiology

authors： Hunke S,Betton JM

更新日期：2003-12-01 00:00:00

abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...

journal_title：Molecular microbiology

authors： Westermayer SA,Fritz G,Gutiérrez J,Megerle JA,Weißl MP,Schnetz K,Gerland U,Rädler JO

更新日期：2016-05-01 00:00:00

abstract：:Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one of the high-Mr PBPs which appears to be altered both in resistant clinical isolates, and in cefotaxime-resistant la...

journal_title：Molecular microbiology

authors： Laible G,Hakenbeck R,Sicard MA,Joris B,Ghuysen JM

更新日期：1989-10-01 00:00:00

abstract：:PspF bound to the psp enhancer activates E sigma54 holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma70-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host ...

journal_title：Molecular microbiology

authors： Jovanovic G,Model P

更新日期：1997-08-01 00:00:00

abstract：:Colletotrichum lagenarium and Magnaporthe grisea are plant pathogenic fungi that produce melanin during the appressorial differentiation stage of conidial germination and during the late stationary phase of mycelial growth. Here, we report the identification of genes for two unique transcription factors, CMR1 (Colleto...

journal_title：Molecular microbiology

authors： Tsuji G,Kenmochi Y,Takano Y,Sweigard J,Farrall L,Furusawa I,Horino O,Kubo Y

更新日期：2000-12-01 00:00:00

abstract：:Transposases mediate transposition first by binding specific DNA end sequences that define a transposable element and then by organizing protein and DNA into a highly structured and stable nucleoprotein 'synaptic' complex. Synaptic complex assembly is a central checkpoint in many transposition mechanisms. The Tn5 syna...

journal_title：Molecular microbiology

authors： Adams CD,Schnurr B,Skoko D,Marko JF,Reznikoff WS

更新日期：2006-12-01 00:00:00

abstract：:The type III secretion system (TTSS) is a modular apparatus assembled by many pathogenic Gram-negative bacteria and is designed to translocate proteins through the bacterial cell wall into the eukaryotic host cell. The conserved components of the TTSS comprise stacks of rings spanning the inner and outer bacterial mem...

journal_title：Molecular microbiology

authors： Daniell SJ,Kocsis E,Morris E,Knutton S,Booy FP,Frankel G

更新日期：2003-07-01 00:00:00