RosE represses Std fimbrial expression in Salmonella enterica serotype Typhimurium.

Abstract:

:The Salmonella enterica serotype Typhimurium (S. typhimurium) genome contains a large repertoire of putative fimbrial operons that remain poorly characterized because they are not expressed in vitro. In this study, insertions that induced expression of the putative stdABCD fimbrial operon were identified from a random bank of transposon mutants by screening with immuno-magnetic particles for ligand expression (SIMPLE). Transposon insertions upstream of csgC and lrhA or within dam, setB and STM4463 (renamed rosE) resulted in expression of StdA and its assembly into fimbrial filaments on the cell surface. RosE is a novel negative regulator of Std fimbrial expression as indicated by its repression of a std::lacZ reporter construct and by binding of the purified protein to a DNA region upstream of the stdA start codon. Expression of Std fimbriae in the rosE mutant resulted in increased attachment of S. typhimurium to human colonic epithelial cell lines (T-84 and CaCo-2). A rosE mutant exhibited a reduced ability to compete with virulent S. typhimurium for colonization of murine organs, while no defect was observed when both competing strains carried a stdAB deletion. These data suggest that a tight control of Std fimbrial expression mediated by RosE is required during host pathogen interaction.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Chessa D,Winter MG,Nuccio SP,Tükel C,Bäumler AJ

doi

10.1111/j.1365-2958.2008.06185.x

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

573-87

issue

3

eissn

0950-382X

issn

1365-2958

pii

MMI6185

journal_volume

68

pub_type

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