Role of rpoS (katF) in oxyR-independent regulation of hydroperoxidase I in Escherichia coli.

Abstract:

:We present evidence showing that rpoS (katF) is a regulator of katG gene transcription in an oxyR-independent manner. Mutation of the rpoS gene in several different Escherichia coli strains caused a significant reduction in catalase HPI activity. In rpoS-delta oxyR double mutants, the level of HPI was considerably lower compared to the delta oxyR parent strain, and was restored when transformed with an rpoS+ plasmid. Overproduction of HPI in oxyR- suppressor strains was greatly diminished after inactivation of the rpoS gene and was accompanied by a substantial increase in sensitivity to menadione. Beta-galactosidase expression from a katG::lacZ promoter was lower in rpoS strains compared to rpoS+ isogenic parents. Several delta oxyR strains had detectable levels of katG transcription that was significantly diminished after rpoS gene inactivation.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Ivanova A,Miller C,Glinsky G,Eisenstark A

doi

10.1111/j.1365-2958.1994.tb01043.x

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

571-8

issue

4

eissn

0950-382X

issn

1365-2958

journal_volume

12

pub_type

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