Abstract:
:A complete understanding of host-parasite interactions must necessarily include the identification and characterization of gene products expressed by both parties during the infectious process. We have developed a new screen to identify bacterial genes that are transcriptionally induced during infection of a host animal. The method is based on pre-selection of strains carrying tnpR operon fusions (encoding resolvase, a site-specific DNA recombinase) which are not expressed in vitro, followed by screening for a subset of these strains that subsequently express resolvase within the host environment. The latter subset was recognized as recombinants that had deleted a resolvase-specific reporter construct. Thirteen transcription units of Vibrio cholerae were identified that were induced during infection in an infant mouse model of cholera. Five of these were predicted to encode polypeptides with diverse functions in metabolism, biosynthesis and motility; one encoded a secreted lipase; two appear to be antisense to genes involved in motility; and five are predicted to encode polypeptides of unknown function. Three of the transcripts were shown to be required for full virulence in infant mice, as assessed by competition experiments.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Camilli A,Mekalanos JJdoi
10.1111/j.1365-2958.1995.mmi_18040671.xsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
671-83issue
4eissn
0950-382Xissn
1365-2958journal_volume
18pub_type
杂志文章abstract::Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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更新日期:2000-08-01 00:00:00
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更新日期:2004-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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journal_title:Molecular microbiology
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doi:10.1111/j.1365-2958.1994.tb01272.x
更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
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pub_type: 评论,杂志文章
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更新日期:2013-02-01 00:00:00
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更新日期:2001-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2016-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1991.tb01861.x
更新日期:1991-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1994.tb00293.x
更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1994.tb00327.x
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2008-02-01 00:00:00