Genetic analysis of the Legionella pneumophila DotB ATPase reveals a role in type IV secretion system protein export.

abstract：:Nitrogen-regulated genes in enterobacteria are positively controlled by the transcriptional activator of σ(N) -dependent promoters NtrC, either directly or indirectly, through the dual regulator Nac. Similar to enterobacteria, gdhA encoding glutamate dehydrogenase from Pseudomonas putida is one of the few genes that i...

journal_title：Molecular microbiology

authors： Hervás AB,Canosa I,Santero E

更新日期：2010-10-01 00:00:00

abstract：:Structural maintenance of chromosome (SMC) and the SMC-interacting kleisin protein families have key functions in the chromosome organization of most organisms. Here, we report that the Bacillus subtilis kleisin, ScpA, can form a ternary complex with the SMC and ScpB proteins in a yeast tri-hybrid assay, supporting th...

journal_title：Molecular microbiology

authors： Dervyn E,Noirot-Gros MF,Mervelet P,McGovern S,Ehrlich SD,Polard P,Noirot P

更新日期：2004-03-01 00:00:00

abstract：:Previous evidence showed that b- and a-type flagellins of Pseudomonas aeruginosa are modified in vivo by phosphorylation at tyrosine. This research was designed to demonstrate phosphorylation of flagellin at tyrosine in vitro. Evidence presented showed that flagellin is labelled by [gamma-32P]-ATP, but not by [alpha-3...

journal_title：Molecular microbiology

authors： South SL,Nichols R,Montie TC

更新日期：1994-06-01 00:00:00

abstract：:Proteus mirabilis is a common cause of urinary tract infection (UTI) in individuals with structural abnormalities or long-term catheterization. The expression of mannose-resistant/Proteus-like (MR/P) fimbria is phase variable because of the inversion of a 251 bp DNA fragment that carries the promoter for the mrp opero...

journal_title：Molecular microbiology

authors： Li X,Lockatell CV,Johnson DE,Mobley HL

更新日期：2002-08-01 00:00:00

abstract：:The 29 kDa protein of pathogenic Entamoeba histolytica is a cysteine-rich surface antigen which we recently characterized by cDNA sequencing and by using monoclonal antibodies which differentiated between pathogenic and non-pathogenic clinical isolates. To determine the structure and biochemical attributes of this pro...

journal_title：Molecular microbiology

authors： Flores BM,Batzer MA,Stein MA,Petersen C,Diedrich DL,Torian BE

更新日期：1993-03-01 00:00:00

abstract：:Listeria monocytogenes grows in the cytosol of mammalian cells and spreads from cell to cell without exiting the intracellular milieu. During cell-cell spread, bacteria become transiently entrapped in double-membrane vacuoles. Escape from these vacuoles is mediated in part by a bacterial phospholipase C (PC-PLC), whos...

journal_title：Molecular microbiology

authors： Marquis H,Hager EJ

更新日期：2000-01-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

journal_title：Molecular microbiology

authors： Ortega DR,Kjaer A,Briegel A

更新日期：2020-09-01 00:00:00

abstract：:Multimer formation reduces plasmid copy number and is an established cause of segregational instability. Nevertheless, it is difficult to rationalize observations that low levels of dimers can cause severe instability, if we assume they are distributed evenly in cell populations. We report here that dimer distribution...

journal_title：Molecular microbiology

authors： Summers DK,Beton CW,Withers HL

更新日期：1993-06-01 00:00:00

abstract：:In contrast to most primary metabolism genes, the genes involved in secondary metabolism and certain nutrient utilization pathways are clustered in fungi. Recently a nuclear protein, LaeA, was found to be required for the transcription of several secondary metabolite gene clusters in Aspergillus nidulans. Here we show...

journal_title：Molecular microbiology

authors： Bok JW,Noordermeer D,Kale SP,Keller NP

更新日期：2006-09-01 00:00:00

abstract：:In cell-free Yersinia pseudotuberculosis culture supernatants, we have chemically characterized three N-acyl homoserine lactone (AHL) molecules, N-octanoyl homoserine lactone (C8-HSL), N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6-HSL) and N-hexanoyl homoserine lactone (C6-HSL). We have identified, cloned and sequence...

journal_title：Molecular microbiology

authors： Atkinson S,Throup JP,Stewart GS,Williams P

更新日期：1999-09-01 00:00:00

abstract：:The Streptomyces wild-type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5' ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0...

journal_title：Molecular microbiology

authors： Volff JN,Altenbuchner J

更新日期：1998-01-01 00:00:00

abstract：:A region of 7.8 kb of the plasmid pMB2 from Enterococcus faecalis S-48 carrying the information necessary for production and immunity of the peptide antibiotic AS-48 has been cloned and sequenced. It contains the as-48A structural gene plus five open reading frames (as-48B, as-48C, as-48C1, as-48D and as-48D1). Beside...

journal_title：Molecular microbiology

authors： Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

更新日期：1998-01-01 00:00:00

abstract：:A reputed iron-responsive region, which contains multiple nuclear protein-binding DNA sequences, was shown previously to regulate iron-inducible transcription of the ap65-1 gene in the protozoan pathogen, Trichomonas vaginalis. These DNA sequences include two overlapping MYB recognition elements (MRE-1/MRE-2r) and thr...

journal_title：Molecular microbiology

authors： Ong SJ,Huang SC,Liu HW,Tai JH

更新日期：2004-06-01 00:00:00

abstract：:Penicillium marneffei is a thermally dimorphic opportunistic human pathogen with a saprophytic filamentous hyphal form at 25 degrees C and a pathogenic unicellular yeast form at 37 degrees C. During infection. P. marneffei yeast cells exist intracellularly in macrophages. To cope with nutrient deprivation during the i...

journal_title：Molecular microbiology

authors： Cánovas D,Andrianopoulos A

更新日期：2006-12-01 00:00:00

abstract：:We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and describe the mutants obtained. A strain carrying a deletion of the chromosomal malE gene, coding for the periplasmic maltose-binding protein (MalE), cannot use maltose unless a wild-type copy ...

journal_title：Molecular microbiology

authors： Seydel A,Gounon P,Pugsley AP

更新日期：1999-11-01 00:00:00

abstract：:The cytokinetic apparatus of bacteria is initially formed by the polymerization of the tubulin-like FtsZ protein into a ring structure at midcell. This so-called Z-ring facilitates the recruitment of many additional proteins to the division site to form the mature divisome machine. Although the assembly pathway leadin...

journal_title：Molecular microbiology

authors： Tsang MJ,Bernhardt TG

更新日期：2015-03-01 00:00:00

abstract：:The predatory bacterium Bdellovibrio bacteriovorus swims rapidly by rotation of a single, polar flagellum comprised of a helical filament of flagellin monomers, contained within a membrane sheath and powered by a basal motor complex. Bdellovibrio collides with, enters and replicates within bacterial prey, a process pr...

journal_title：Molecular microbiology

authors： Lambert C,Evans KJ,Till R,Hobley L,Capeness M,Rendulic S,Schuster SC,Aizawa S,Sockett RE

更新日期：2006-04-01 00:00:00

abstract：:The structural complexity of a ligand in association with the molecular chaperones SecB and SecA was investigated using three species of precursor maltose-binding protein, which differ in their stability as a result of an amino acid substitution in each that affects the rate of folding of the polypeptide. In the prese...

journal_title：Molecular microbiology

authors： de Cock H,Randall LL

更新日期：1998-01-01 00:00:00

abstract：:The fimbrial and afimbrial adhesins of the Dr family mediate the adherence of uropathogenic and diarrhoea-associated Escherichia coli to decay-accelerating factor (DAF) present on erythrocytes and other cell types. The Dr haemagglutinin binds type IV collagen and, unlike other members of the Dr family, mediates an adh...

journal_title：Molecular microbiology

authors： Carnoy C,Moseley SL

更新日期：1997-01-01 00:00:00

abstract：:The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of antimonite [Sb(III)], an activated form of Pentostam or Glucantime. Downregulation of LmAQP1 provides resistance to trivalent antimony compounds and increased expression of LmAQP1 in drug-resistant parasites can reverse the resistance. ...

journal_title：Molecular microbiology

authors： Uzcategui NL,Zhou Y,Figarella K,Ye J,Mukhopadhyay R,Bhattacharjee H

更新日期：2008-12-01 00:00:00

abstract：:Phytopathogenic bacteria possess a large number of genes that allow them to grow and cause disease on plants. Many of these genes should be induced when the bacteria come in contact with plant tissue. We used a modified in vivo expression technology (IVET) approach to identify genes from the plant pathogen Pseudomonas...

journal_title：Molecular microbiology

authors： Boch J,Joardar V,Gao L,Robertson TL,Lim M,Kunkel BN

更新日期：2002-04-01 00:00:00

abstract：:bfp, the structural gene of the major repeating bundle-forming pilus (BFP) subunit, was cloned from the enteroadherent factor (EAF) plasmid of enteropathogenic Escherichia coli (EPEC) strain B171 (O111:NM). The bfp open reading frame encoded a 193-amino-acid protein; comparison of this sequence with the biochemically ...

journal_title：Molecular microbiology

authors： Sohel I,Puente JL,Murray WJ,Vuopio-Varkila J,Schoolnik GK

更新日期：1993-02-01 00:00:00

abstract：:Experimental evidence suggests that apicomplexan parasites possess bipartite promoters with basal and regulated cis-elements similar to other eukaryotes. Using a dual luciferase model adapted for recombinational cloning and use in Toxoplasma gondii, we show that genomic regions flanking 16 parasite genes, which encomp...

journal_title：Molecular microbiology

authors： Behnke MS,Radke JB,Smith AT,Sullivan WJ Jr,White MW

更新日期：2008-06-01 00:00:00

abstract：:Transcription of flagellar genes in Caulobacter crecentus is programmed to occur during the predivisional stage of the cell cycle. The mechanism of activation of Class II flagellar genes, the highest identified genes in the Caulobacter flagellar hierarchy, is unknown. As a step toward understanding this process, we ha...

journal_title：Molecular microbiology

authors： Stephens CM,Shapiro L

更新日期：1993-09-01 00:00:00

abstract：:The surface-located fibrinogen-binding protein (clumping factor; ClfA) of Staphylococcus aureus has an unusual dipeptide repeat linking the ligand binding domain to the wall-anchored region. Southern blotting experiments revealed several other loci in the S. aureus Newman genome that hybridized to a probe comprising D...

journal_title：Molecular microbiology

authors： Ní Eidhin D,Perkins S,Francois P,Vaudaux P,Höök M,Foster TJ

更新日期：1998-10-01 00:00:00

abstract：:Pseudomonas aeruginosa can express two distinct forms of lipopolysaccharide (LPS), called A-band and B-band. As an attempt to understand the molecular biology of the synthesis and regulation of these LPS antigens, a recombinant plasmid, pFV3, containing genes for A-band expression was isolated previously. In the prese...

journal_title：Molecular microbiology

authors： Lightfoot J,Lam JS

更新日期：1993-05-01 00:00:00

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:An msDNA operon, consisting of genes for msDNA and a reverse transcriptase, is present in Escherichia coliB and absent from E. coliK12. We have found that the msDNA operon is located on a DNA fragment, longer than 15kb, that is absent from E. coliK12. Using conjugation, P1 transduction, and nucleic acid hybridization ...

journal_title：Molecular microbiology

authors： Lim D,Maas WK

更新日期：1990-12-01 00:00:00

abstract：:Erwinia chrysanthemi, a phytopathogenic bacterium, produces a protease inhibitor which is a low-molecular-weight, heat-stable protein. In addition to its action on the three E. chrysanthemi extracellular proteases A, B and C, it also strongly inhibits the 50 kD extracellular protease of Serratia marcescens. Its struct...

journal_title：Molecular microbiology

authors： Létoffé S,Delepelaire P,Wandersman C

更新日期：1989-01-01 00:00:00

abstract：:Ribonucleotide reductases supply cells with their deoxyribonucleotides. Three enzyme types are known, classes I, II and III. Class II enzymes are anaerobic whereas class I enzymes are aerobic, and so class I and II enzymes are often produced by the same organism under opposing oxygen regimes. Escherichia coli contains...

journal_title：Molecular microbiology

authors： Andrews SC

更新日期：2011-04-01 00:00:00