Evidence that acetyl phosphate functions as a global signal during biofilm development.


:We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observations that we published previously (Prüss and Wolfe, 1994, Mol Microbiol 12: 973-984). In contrast, genes whose expression correlated positively with the ability to synthesize acP (i.e. positively regulated genes) include those for type 1 pilus assembly, colanic acid (capsule) biosynthesis and certain stress effectors. To our knowledge, this constitutes the first report that these genes may respond to the status of the intracellular acP pool. Previously, other researchers have implicated flagella, type 1 pili, capsule and diverse stress effectors in the formation of biofilms. We therefore tested whether cells altered in their ability to metabolize acP could construct normal biofilms, and found that they could not. Cells defective for the production of acP and cells defective for the degradation of acP could both form biofilms, but these biofilms exhibited characteristics substantially different from each other and from biofilms formed by their wild-type parent. We confirmed the role of individual cell surface structures, the expression of which appears to correlate with acP levels, in fim or fli mutants that cannot assemble type 1 pili or flagella respectively. Thus, the information gained by expression profiling of cells with altered acP metabolism indicates that acP may help to co-ordinate the expression of surface structures and cellular processes involved in the initial stages of wild-type biofilm development.


Mol Microbiol


Molecular microbiology


Wolfe AJ,Chang DE,Walker JD,Seitz-Partridge JE,Vidaurri MD,Lange CF,Prüss BM,Henk MC,Larkin JC,Conway T




Has Abstract


2003-05-01 00:00:00














  • Redox and antioxidant systems of the malaria parasite Plasmodium falciparum.

    abstract::The malaria parasite Plasmodium falciparum is highly adapted to cope with the oxidative stress to which it is exposed during the erythrocytic stages of its life cycle. This includes the defence against oxidative insults arising from the parasite's metabolism of haemoglobin which results in the formation of reactive ox...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Müller S

    更新日期:2004-09-01 00:00:00

  • The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Garrett JM

    更新日期:1997-11-01 00:00:00

  • The rpf gene of Micrococcus luteus encodes an essential secreted growth factor.

    abstract::Micrococcus luteus secretes a small protein called Rpf, which has autocrine and paracrine signalling functions and is required for the resuscitation of dormant cells. Originally isolated from the supernatant of actively growing cultures, Rpf was also detected on the surface of actively growing bacteria. Most molecules...

    journal_title:Molecular microbiology

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    authors: Mukamolova GV,Turapov OA,Kazarian K,Telkov M,Kaprelyants AS,Kell DB,Young M

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  • Uropathogenic Escherichia coli use d-serine deaminase to modulate infection of the murine urinary tract.

    abstract::Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1. An epidemiological survey demonstrated that urine isolates of E. coli are more likely to catabolise d-serine via expression...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Roesch PL,Redford P,Batchelet S,Moritz RL,Pellett S,Haugen BJ,Blattner FR,Welch RA

    更新日期:2003-07-01 00:00:00

  • Characterizing the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibrio bacteriovorus.

    abstract::The predatory bacterium Bdellovibrio bacteriovorus swims rapidly by rotation of a single, polar flagellum comprised of a helical filament of flagellin monomers, contained within a membrane sheath and powered by a basal motor complex. Bdellovibrio collides with, enters and replicates within bacterial prey, a process pr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lambert C,Evans KJ,Till R,Hobley L,Capeness M,Rendulic S,Schuster SC,Aizawa S,Sockett RE

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  • Mapping of the msDNA operon in the chromosome of Escherichia coli B.

    abstract::An msDNA operon, consisting of genes for msDNA and a reverse transcriptase, is present in Escherichia coliB and absent from E. coliK12. We have found that the msDNA operon is located on a DNA fragment, longer than 15kb, that is absent from E. coliK12. Using conjugation, P1 transduction, and nucleic acid hybridization ...

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    authors: Lim D,Maas WK

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  • The purified SoxABCD quinol oxidase complex of Sulfolobus acidocaldarius contains a novel haem.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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  • Regulation of quorum sensing in Vibrio harveyi by LuxO and sigma-54.

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    pub_type: 杂志文章


    authors: Lilley BN,Bassler BL

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  • The two faces of Janus: virulence gene regulation by CovR/S in group A streptococci.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Churchward G

    更新日期:2007-04-01 00:00:00

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    abstract::Fibronectin-binding proteins (FnBPs) are thought to be important for the attachment of Staphylococcus aureus during infection. The regulation of the genes fnbA and fnbB by the global regulatory loci sar and agr was examined using site-specific regulatory mutants of S. aureus strain Newman. The results from binding ass...

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    pub_type: 杂志文章


    authors: Wolz C,Pöhlmann-Dietze P,Steinhuber A,Chien YT,Manna A,van Wamel W,Cheung A

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  • Mechanisms for maintaining cell shape in rod-shaped Gram-negative bacteria.

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    authors: Furchtgott L,Wingreen NS,Huang KC

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    authors: Liu B,Persons L,Lee L,de Boer PA

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    authors: Ieva R,Skillman KM,Bernstein HD

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    authors: Papenfort K,Pfeiffer V,Lucchini S,Sonawane A,Hinton JC,Vogel J

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    authors: Mora L,Heurgué-Hamard V,Champ S,Ehrenberg M,Kisselev LL,Buckingham RH

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  • Light-dependent gene activation in Aspergillus nidulans is strictly dependent on phytochrome and involves the interplay of phytochrome and white collar-regulated histone H3 acetylation.

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    authors: Coros CJ,Landthaler M,Piazza CL,Beauregard A,Esposito D,Perutka J,Lambowitz AM,Belfort M

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  • Streptococcus mutans autolysin AtlA is a fibronectin-binding protein and contributes to bacterial survival in the bloodstream and virulence for infective endocarditis.

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    authors: Wang E,Bauer MC,Rogstam A,Linse S,Logan DT,von Wachenfeldt C

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    authors: Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

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    pub_type: 杂志文章


    authors: Chan CH,Garrity J,Crosby HA,Escalante-Semerena JC

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  • Activation of biosynthetic gene clusters by the global transcriptional regulator TRI6 in Fusarium graminearum.

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    pub_type: 杂志文章


    authors: Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Yao R,Burr DH,Doig P,Trust TJ,Niu H,Guerry P

    更新日期:1994-12-01 00:00:00

  • Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs.

    abstract::Quinone-based compounds have been exploited to treat infectious diseases and cancer, with such chemicals often functioning as inhibitors of key metabolic pathways or as prodrugs. Here, we screened an aziridinyl 1,4-benzoquinone (ABQ) library against the causative agents of trypanosomiasis, and cutaneous leishmaniasis,...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

    更新日期:2017-10-01 00:00:00

  • Requirement of the VanY and VanX D,D-peptidases for glycopeptide resistance in enterococci.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Arthur M,Depardieu F,Cabanié L,Reynolds P,Courvalin P

    更新日期:1998-11-01 00:00:00

  • Sequence, localization and function of the invasin protein of Yersinia enterocolitica.

    abstract::The inv locus of Yersinia enterocolitica is sufficient to convert a non-invasive Escherichia coli K12 strain into a microorganism that is able to penetrate cultured mammalian cells. The nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Young VB,Miller VL,Falkow S,Schoolnik GK

    更新日期:1990-07-01 00:00:00

  • Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism.

    abstract::Soupene et al. [J. Bacteriol. (2003) 185 5611-5626] made the unexpected observation that the presence of a mutation, in the gene for the N-acetylglucosamine repressor, nagC, increased the growth rate of Escherichia coli MG1655 on galactose, an unrelated sugar. We have found that NagC, binds to a single, high-affinity ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: El Qaidi S,Allemand F,Oberto J,Plumbridge J

    更新日期:2009-01-01 00:00:00

  • Determinants of segregational stability of the linear plasmid-prophage N15 of Escherichia coli.

    abstract::N15 is a bacteriophage of Escherichia coli that resembles lambda, but, unlike lambda, it lysogenizes as a linear plasmid. We show that stable maintenance of this unusual plasmid-prophage depends on the parA and parB genes, relatives of the partition genes of F and P1 plasmids. ParB of N15, like its F- and P1-encoded h...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Grigoriev PS,Lobocka MB

    更新日期:2001-10-01 00:00:00

  • Regulation of plasmid R1 replication: PcnB and RNase E expedite the decay of the antisense RNA, CopA.

    abstract::The replication frequency of plasmid R1 is controlled by an unstable antisense RNA, CopA, which, by binding to its complementary target, blocks translation of the replication rate-limiting protein RepA. Since the degree of inhibition is directly correlated with the intracellular concentration of CopA, factors affectin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Söderbom F,Binnie U,Masters M,Wagner EG

    更新日期:1997-11-01 00:00:00