HobA--a novel protein involved in initiation of chromosomal replication in Helicobacter pylori.

Abstract:

:Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to any of these factors. Here, we describe HobA, a novel protein essential for initiation of H. pylori chromosome replication, which is conserved among, and unique to, epsilon proteobacteria. We demonstrate that HobA interacts specifically via DnaA with the oriC-DnaA complex. We postulate that HobA is essential for correct formation and stabilization of the orisome by facilitating the spatial positioning of DnaA at oriC. Consistent with its function, overexpression of hobA had no effect on growth of H. pylori, whereas depletion of HobA led to growth arrest and failure to initiate replication. In conclusion, HobA may be the first identified of a new group of initiation factors common to epsilon proteobacteria.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Zawilak-Pawlik A,Kois A,Stingl K,Boneca IG,Skrobuk P,Piotr J,Lurz R,Zakrzewska-Czerwińska J,Labigne A

doi

10.1111/j.1365-2958.2007.05853.x

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

979-94

issue

4

eissn

0950-382X

issn

1365-2958

pii

MMI5853

journal_volume

65

pub_type

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