Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

abstract：:Phaseolotoxin, a phytotoxin of Pseudomonas syringae pv. phaseolicola, is produced at 18 degrees C but not at 28 degrees C. Here we report that a fragment (24.4 kb) cloned from the wild-type strain, which does not harbour a gene(s) involved in phaseolotoxin biosynthesis, abolishes this thermoregulation in the wild type...

journal_title：Molecular microbiology

authors： Rowley KB,Clements DE,Mandel M,Humphreys T,Patil SS

更新日期：1993-05-01 00:00:00

abstract：:We report the construction and the expression in Escherichia coli of three different fusion genes encoding the extended human IgG3 hinge region (Hi) fused in-phase to the C-terminal end of bacterial TEM1 beta-lactamase (Bla). In the first fusion gene blahi, TEM1 beta-lactamase (Bla). In the first fusion gene blahi, th...

journal_title：Molecular microbiology

authors： De Sutter K,Remaut E,Fiers W

更新日期：1992-08-01 00:00:00

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:Molecular analyses were applied to the genes encoding variants of the serosubtyping antigen, the class 1 outer membrane protein (PorA), from 55 serogroup A Neisseria meningitidis strains. These genes were evolutionarily stable and exhibited a limited range of genetic variation, primarily generated by recombination. Tr...

journal_title：Molecular microbiology

authors： Suker J,Feavers IM,Achtman M,Morelli G,Wang JF,Maiden MC

更新日期：1994-04-01 00:00:00

abstract：:The chromosomal gef gene of Escherichia coli is a member of the gef gene family which encodes strongly toxic proteins of about 50 amino acids. We demonstrate here that the Gef protein is detectable by anti-peptide antibodies. Furthermore, we show that Gef is anchored in the cytoplasmic membrane by the N-terminal part ...

journal_title：Molecular microbiology

authors： Poulsen LK,Refn A,Molin S,Andersson P

更新日期：1991-07-01 00:00:00

abstract：:In response to nitrate availability, Escherichia coli regulates the synthesis of a number of enzymes involved in anaerobic respiration and fermentation. When nitrate is present, nitrate reductase (narGHJI) gene expression is induced, while expression of the DMSO/TMAO reductase (dmsABC), fumarate reductase (frdABCD) an...

journal_title：Molecular microbiology

authors： Chiang RC,Cavicchioli R,Gunsalus RP

更新日期：1992-07-01 00:00:00

abstract：SUMMARY:The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated b...

journal_title：Molecular microbiology

authors： Davidov E,Kaufmann G

更新日期：2008-09-01 00:00:00

abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...

journal_title：Molecular microbiology

authors： Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jr

更新日期：1995-06-01 00:00:00

abstract：:Photoregulation of pigmentation during complementary chromatic acclimation (CCA) is well studied in Fremyella diplosiphon; however, mechanistic insights into the CCA-associated morphological changes are still emerging. F. diplosiphon cells are rectangular under green light (GL), whereas cells are smaller and spherical...

journal_title：Molecular microbiology

authors： Singh SP,Montgomery BL

更新日期：2014-07-01 00:00:00

abstract：:VP4, the major structural protein of the haloarchaeal pleomorphic virus, HRPV-1, is glycosylated. To define the glycan structure attached to this protein, oligosaccharides released by β-elimination were analysed by mass spectrometry and nuclear magnetic resonance spectroscopy. Such analyses showed that the major VP4-d...

journal_title：Molecular microbiology

authors： Kandiba L,Aitio O,Helin J,Guan Z,Permi P,Bamford DH,Eichler J,Roine E

更新日期：2012-05-01 00:00:00

abstract：:Cytoadherence to the vaginal epithelium is a critical step in infection by the eukaryotic flagellate Trichomonas vaginalis. Four trichomonad surface proteins (AP65, AP51, AP33 and AP23) mediate cytoadherence. The cDNA encoding the AP65 adhesin was isolated from a phagemid cDNA expression library by screening with anti...

journal_title：Molecular microbiology

authors： Alderete JF,O'Brien JL,Arroyo R,Engbring JA,Musatovova O,Lopez O,Lauriano C,Nguyen J

更新日期：1995-07-01 00:00:00

abstract：:A series of lipoproteins was detected in the membrane fraction of Yersinia enterocolitica W227, a typical strain from serotype O:9. At least two of them, YlpA and YlpB, are encoded by the pYV plasmid. The sequence of ylpA reveals the presence of a typical lipoprotein signal peptide. The mature YlpA protein would be 22...

journal_title：Molecular microbiology

authors： China B,Michiels T,Cornelis GR

更新日期：1990-09-01 00:00:00

abstract：:Disulfide bonds are found in many proteins associated with the cell wall of Escherichia coli, and for some of these proteins the disulfide bond is critical to their stability and function. One protein found to contain a disulfide bond is the essential cell division protein FtsN, but the importance of this bond to the ...

journal_title：Molecular microbiology

authors： Meehan BM,Landeta C,Boyd D,Beckwith J

更新日期：2017-02-01 00:00:00

abstract：:The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctional chorismatase that hydroly...

journal_title：Molecular microbiology

authors： Zhou L,Wang JY,Wu J,Wang J,Poplawsky A,Lin S,Zhu B,Chang C,Zhou T,Zhang LH,He YW

更新日期：2013-01-01 00:00:00

abstract：:Regulation of polar development and cell division in Caulobacter crescentus relies on the dynamic localization of several proteins to cell poles at specific stages of the cell cycle. The polar organelle development protein, PodJ, is required for the synthesis of the adhesive holdfast and pili. Here we show the cell cy...

journal_title：Molecular microbiology

authors： Hinz AJ,Larson DE,Smith CS,Brun YV

更新日期：2003-02-01 00:00:00

abstract：:The chromosome ends of human malaria parasites harbour many genes encoding proteins that are exported to the surface of infected red cells, often being involved in host-parasite interactions and immune evasion. Unlike other murine malaria parasites Plasmodium chabaudi undergoes antigenic variation during passage in th...

journal_title：Molecular microbiology

authors： Fischer K,Chavchich M,Huestis R,Wilson DW,Kemp DJ,Saul A

更新日期：2003-06-01 00:00:00

abstract：:Teichoic acids (TAs) are the most abundant glycopolymers in the cell wall of Listeria, an opportunistic Gram-positive pathogen that causes severe foodborne infections. Two different structural classes of Listeria TA exist: the polyribitolphosphate-based wall teichoic acid (WTA) that is covalently anchored to the pepti...

journal_title：Molecular microbiology

authors： Sumrall ET,Keller AP,Shen Y,Loessner MJ

更新日期：2020-03-01 00:00:00

abstract：:Post-transcriptional repression of porin synthesis has emerged as a major function of Hfq-dependent, small non-coding RNAs (sRNAs). Many enterobacteria express OmpX-like porins, a family of outer membrane proteins whose physiological roles and structural properties have been studied intensively. While regulatory sRNAs...

journal_title：Molecular microbiology

authors： Papenfort K,Pfeiffer V,Lucchini S,Sonawane A,Hinton JC,Vogel J

更新日期：2008-05-01 00:00:00

abstract：:Strain 1470 is the standard typing strain for serogroup F of Clostridium difficile containing both toxin genes, toxA-1470 and toxB-1470. A polymerase chain reaction (PCR)-based approach to the sequencing of the total toxB-1470 gene identified an open reading frame (ORF) of 7104 nucleotides. In comparison with the prev...

journal_title：Molecular microbiology

authors： von Eichel-Streiber C,Meyer zu Heringdorf D,Habermann E,Sartingen S

更新日期：1995-07-01 00:00:00

abstract：:BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that BldD also activates antibiotic production are provided. In Streptomyces roseosporus (which produces daptomycin widely used for treatment of human infections), deletion of bldD notably reduced dap...

journal_title：Molecular microbiology

authors： Yan H,Lu X,Sun D,Zhuang S,Chen Q,Chen Z,Li J,Wen Y

更新日期：2020-01-01 00:00:00

abstract：:Stimulation of Escherichia coli with acetate elevates the acetylation level of the chemotaxis response regulator CheY. This elevation, in an unknown mechanism, activates CheY to generate clockwise rotation. Here, using quantitative selective reaction monitoring mass spectrometry and high-resolution targeted mass spect...

journal_title：Molecular microbiology

authors： Fraiberg M,Afanzar O,Cassidy CK,Gabashvili A,Schulten K,Levin Y,Eisenbach M

更新日期：2015-01-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen that colonizes ∼ 50% of the world's population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. The numerous side effects of available treatments and the emergence of antibiotic resistant strains are severe concerns that justify further resear...

journal_title：Molecular microbiology

authors： Lester J,Kichler S,Oickle B,Fairweather S,Oberc A,Chahal J,Ratnayake D,Creuzenet C

更新日期：2015-04-01 00:00:00

abstract：:Pseudomonas aeruginosa OprD is a 420-amino-acid protein that facilitates the uptake of basic amino acids, imipenem and gluconate across the outer membrane. OprD was the first specific porin that could be aligned with members of the non-specific porin super-family. Utilizing multiple alignments in conjugation with stru...

journal_title：Molecular microbiology

authors： Huang H,Jeanteur D,Pattus F,Hancock RE

更新日期：1995-06-01 00:00:00

abstract：:Transposon Tn 1546 confers resistance to glycopeptide antibiotics in enterococci and encodes two D,D-peptidases (VanX and VanY) in addition to the enzymes for the synthesis of D-alanyl-D-lactate (D-Ala-D-Lac). VanY was produced in the baculovirus expression system and purified as a proteolytic fragment that lacked the...

journal_title：Molecular microbiology

authors： Arthur M,Depardieu F,Cabanié L,Reynolds P,Courvalin P

更新日期：1998-11-01 00:00:00

abstract：:A series of internal deletions in the lktA gene of Pasteurella haemolytica has been constructed. All of the deletions eliminated the lytic activity of the leukotoxin towards the bovine lymphoma cell line, BL-3. Deletions removing segments of the amino-proximal hydrophobic region, which is thought to constitute an esse...

journal_title：Molecular microbiology

authors： Cruz WT,Young R,Chang YF,Struck DK

更新日期：1990-11-01 00:00:00

abstract：:FlhD and FlhC are the transcriptional activators of the flagellar regulon. The heterotetrameric complex formed by these two proteins activates the transcription of the class II flagellar genes. The flagellar regulon consists not only of flagellar genes, but also of the chemotactic genes and some receptor proteins. Rec...

journal_title：Molecular microbiology

authors： Campos A,Matsumura P

更新日期：2001-02-01 00:00:00

abstract：:The two-component regulatory system PhoPQ has been shown to regulate the expression of virulence factors in a number of bacterial species. For one such virulence factor, lipopolysaccharide (LPS), the PhoPQ system has been shown to regulate structural modifications in Salmonella enterica var Typhimurium. In Yersinia pe...

journal_title：Molecular microbiology

authors： Hitchen PG,Prior JL,Oyston PC,Panico M,Wren BW,Titball RW,Morris HR,Dell A

更新日期：2002-06-01 00:00:00

abstract：:A bioassay was developed to identify stimuli that promote the transcriptional induction of the algD operon for alginate biosynthesis in Pseudomonas aeruginosa. Strain PAO1 carried the algD promoter fused to a chloramphenicol acetyl-transferase cartridge (PalgD-cat), and > 50 compounds were tested for promoting chloram...

journal_title：Molecular microbiology

authors： Wood LF,Leech AJ,Ohman DE

更新日期：2006-10-01 00:00:00

abstract：:A number of eukaryotic proteins are already known to orchestrate key steps of mRNA metabolism and translation via interactions with the 5' m7GpppN cap. We have characterized a new type of histidine triad (HIT) motif protein (Nhm1) that co-purifies with the cap-binding complex eIF4F of Schizosaccharomyces pombe. Nhm1 i...

journal_title：Molecular microbiology

authors： Salehi Z,Geffers L,Vilela C,Birkenhäger R,Ptushkina M,Berthelot K,Ferro M,Gaskell S,Hagan I,Stapley B,McCarthy JE

更新日期：2002-10-01 00:00:00

abstract：:A gapA-pgk gene tandem coding the glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase, is most frequently found in bacteria. However, in Enterobacteriaceae, gapA is replaced by an epd open reading frame (ORF) coding an erythrose-4-phosphate dehydrogenase and an fbaA ORF coding the class II fructose-...

journal_title：Molecular microbiology

authors： Bardey V,Vallet C,Robas N,Charpentier B,Thouvenot B,Mougin A,Hajnsdorf E,Régnier P,Springer M,Branlant C

更新日期：2005-09-01 00:00:00