The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins.

abstract：:The liver stage is the first stage of the malaria parasite that replicates in the vertebrate host. However, little is known about the interplay between the parasite liver stage and its host cell, the hepatocyte. In this study, we identified an exported protein that has a critical role in parasite development in host h...

journal_title：Molecular microbiology

authors： Orito Y,Ishino T,Iwanaga S,Kaneko I,Kato T,Menard R,Chinzei Y,Yuda M

更新日期：2013-01-01 00:00:00

abstract：:The chromosomal DpnII gene cassette of Streptococcus pneumoniae encodes two methyltransferases and an endonuclease. One methyltransferase acts on double-stranded and the other on single-stranded DNA. Two mRNAs are transcribed from the cassette. One, a SigA promoter transcript, includes all three genes; the other inclu...

journal_title：Molecular microbiology

authors： Lacks SA,Ayalew S,de la Campa AG,Greenberg B

更新日期：2000-03-01 00:00:00

abstract：:Many fungi undergo a morphological transition to filamentous growth in response to limiting nutrient conditions. Constitutively elongated Saccharomyces cerevisiae mutants (elm) have been isolated; the ELM1 gene encodes a putative serine/threonine protein kinase. A novel allele, elm1-15, has been isolated in an S288C-d...

journal_title：Molecular microbiology

authors： Garrett JM

更新日期：1997-11-01 00:00:00

abstract：:Three u.v.-induced mutants of the purine-cytosine permease gene of Saccharomyces cerevisiae, with altered apparent Michaelis constant of transport (Kmapp), were cloned and sequenced. One of the mutants had extensive nucleotide replacement, whereas the other two had a single mutation. To evaluate the contribution of th...

journal_title：Molecular microbiology

authors： Bloch JC,Sychrova H,Souciet JL,Jund R,Chevallier MR

更新日期：1992-10-01 00:00:00

abstract：:Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here,...

journal_title：Molecular microbiology

authors： Davis R,Écija-Conesa A,Gallego-Jara J,de Diego T,Filippova EV,Kuffel G,Anderson WF,Gibson BW,Schilling B,Canovas M,Wolfe AJ

更新日期：2018-01-01 00:00:00

abstract：:Lipid metabolism in Trypanosoma brucei, the causative agent of African sleeping sickness, differs from its human host in several fundamental ways. This has lead to the validation of a plethora of novel drug targets, giving hope of novel chemical intervention against this neglected disease. Cytidine diphosphate diacylg...

journal_title：Molecular microbiology

authors： Lilley AC,Major L,Young S,Stark MJ,Smith TK

更新日期：2014-05-01 00:00:00

abstract：:The mechanisms by which micro-organisms sense and internalize extracellular pH signals are not completely understood. One example of a known external pH-sensing process is the fungal-specific Rim/Pal signal transduction pathway. Fungi, such as the opportunistic pathogen Cryptococcus neoformans, use Rim signaling to se...

journal_title：Molecular microbiology

authors： Brown HE,Ost KS,Esher SK,Pianalto KM,Saelens JW,Guan Z,Andrew Alspaugh J

更新日期：2018-08-01 00:00:00

abstract：:PilO of Pseudomonas aeruginosa 1244 catalyses the attachment of an O-antigen repeating unit to the beta-carbon of the pilin C-terminal residue, a serine. The present study was conducted to locate the regions of this enzyme important in catalysis and to establish the cellular location of the pilin glycosylation reactio...

journal_title：Molecular microbiology

authors： Qutyan M,Paliotti M,Castric P

更新日期：2007-12-01 00:00:00

abstract：:Clostridium difficile, an emerging nosocomial pathogen of increasing clinical significance, produces two large protein toxins that are responsible for the cellular damage associated with the disease. The precise mechanisms by which toxin synthesis is regulated in response to environmental change have yet to be discove...

journal_title：Molecular microbiology

authors： Matamouros S,England P,Dupuy B

更新日期：2007-06-01 00:00:00

abstract：:The repABC replicons contain an operon encoding the initiator protein (RepC) and partitioning proteins (RepA and RepB). The latter two proteins negatively regulate the transcription of the operon. In this article we have identified two novel regulatory elements, located within the conserved repB-repC intergenic sequen...

journal_title：Molecular microbiology

authors： Venkova-Canova T,Soberón NE,Ramírez-Romero MA,Cevallos MA

更新日期：2004-12-01 00:00:00

abstract：:It was demonstrated recently that the SeqA protein, a main negative regulator of Escherichia coli chromosome replication initiation, is also a specific transcription factor. SeqA specifically activates the bacteriophage lambda pR promoter while revealing no significant effect on the activity of another lambda promoter...

journal_title：Molecular microbiology

authors： Słomińska M,Konopa G,Ostrowska J,Kedzierska B,Wegrzyn G,Wegrzyn A

更新日期：2003-03-01 00:00:00

abstract：:In spite of displaying the most extreme dormancy and resistance properties known among living systems, bacterial endospores retain an alert environment-sensing mechanism that can respond within seconds to the presence of specific germinants. This germination response is triggered in the absence of both germinant and g...

journal_title：Molecular microbiology

authors： Foster SJ,Johnstone K

更新日期：1990-01-01 00:00:00

abstract：:Billions of years of evolution have yielded today's complex metabolic networks driven by efficient and highly specialized enzymes. In contrast, the metabolism of the earliest cellular life forms was likely much simpler with only a few enzymes of comparatively low activity. It has been speculated that these early enzym...

journal_title：Molecular microbiology

authors： Seelig B

更新日期：2017-08-01 00:00:00

abstract：:The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

journal_title：Molecular microbiology

authors： Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

更新日期：2011-08-01 00:00:00

abstract：:The bvg locus contains two genes, bvgA and bvgS, which control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli. We determined the nucleotide sequence of the bvg loci of Bor...

journal_title：Molecular microbiology

authors： Aricò B,Scarlato V,Monack DM,Falkow S,Rappuoli R

更新日期：1991-10-01 00:00:00

abstract：:Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it is admitted that any accessible RNA 3' end can be tagged by a poly(A) tail for decay. However, we do not have yet an overall view of the population of polyadenylated m...

journal_title：Molecular microbiology

authors： Maes A,Gracia C,Hajnsdorf E,Régnier P

更新日期：2012-01-01 00:00:00

abstract：:Transposases mediate transposition first by binding specific DNA end sequences that define a transposable element and then by organizing protein and DNA into a highly structured and stable nucleoprotein 'synaptic' complex. Synaptic complex assembly is a central checkpoint in many transposition mechanisms. The Tn5 syna...

journal_title：Molecular microbiology

authors： Adams CD,Schnurr B,Skoko D,Marko JF,Reznikoff WS

更新日期：2006-12-01 00:00:00

abstract：:Gyrase-mediated hypernegative supercoiling is one manifestation of R-loop formation, a phenomenon that is normally suppressed by topoisomerase I (topA) in Escherichia coli. Overproduction of RNase HI (rnhA), an enzyme that removes the RNA moiety of R-loops, prevents hypernegative supercoiling and allows growth of topA...

journal_title：Molecular microbiology

authors： Usongo V,Nolent F,Sanscartier P,Tanguay C,Broccoli S,Baaklini I,Drlica K,Drolet M

更新日期：2008-08-01 00:00:00

abstract：:In Pseudomonas aeruginosa three type VI secretion systems (T6SSs) coexist, called H1- to H3-T6SSs. Several T6SS components are proposed to be part of a macromolecular complex resembling the bacteriophage tail. The T6SS protein HsiE1 (TagJ) is unique to the H1-T6SS and absent from the H2- and H3-T6SSs. We demonstrate t...

journal_title：Molecular microbiology

authors： Lossi NS,Manoli E,Simpson P,Jones C,Hui K,Dajani R,Coulthurst SJ,Freemont P,Filloux A

更新日期：2012-10-01 00:00:00

abstract：:The spoIIIG gene encodes a sigma factor that determines prespore-specific gene expression during sporulation in Bacillus subtilis. Correct spatial and temporal expression of the spoIIIG gene depends on a number of other sporulation (spo) genes, but only one of these genes, spoIIIE, has a specific effect on spoIIIG exp...

journal_title：Molecular microbiology

authors： Foulger D,Errington J

更新日期：1989-09-01 00:00:00

abstract：:Leishmania parasites target macrophages in their mammalian hosts and proliferate within the mature phagolysosome compartment of these cells. Intracellular amastigote stages are dependent on sugars as a major carbon source in vivo, but retain the capacity to utilize other carbon sources. To investigate whether amastigo...

journal_title：Molecular microbiology

authors： Saunders EC,Naderer T,Chambers J,Landfear SM,McConville MJ

更新日期：2018-04-01 00:00:00

abstract：:The important human pathogen Streptococcus pyogenes (the group A streptococcus or GAS) causes diseases ranging from mild, self-limiting pharyngitis to severe invasive infections. Regulation of the expression of GAS genes in response to specific environmental differences within the host is probably key in determining t...

journal_title：Molecular microbiology

authors： Opdyke JA,Scott JR,Moran CP Jr

更新日期：2001-10-01 00:00:00

abstract：:The best studied role of ubiquitination is to mark proteins for destruction by the proteasome but, in addition, it has recently been shown to promote macromolecular assembly and function, and alter protein function, thus playing a regulatory role distinct from protein degradation. Deubiquinating enzymes, the ubiquitin...

journal_title：Molecular microbiology

authors： Lockington RA,Kelly JM

更新日期：2001-06-01 00:00:00

abstract：:Aspergillus nidulans was used as a model organism to investigate the fungal propionate metabolism and the mechanism of growth inhibition by propionate. The fungus is able to grow slowly on propionate as sole carbon and energy source. Propionate is oxidized to pyruvate via the methylcitrate cycle. The key enzyme methyl...

journal_title：Molecular microbiology

authors： Brock M,Fischer R,Linder D,Buckel W

更新日期：2000-03-01 00:00:00

abstract：:Bacteria differ from eukaryotes by having the enzyme DNA gyrase, which catalyses the ATP-dependent negative supercoiling of DNA. Negative supercoils are essential for condensing chromosomes into an interwound (plectonemic) and branched structure known as the nucleoid. Topo-1 removes excess supercoiling in an ATP-indep...

journal_title：Molecular microbiology

authors： Booker BM,Deng S,Higgins NP

更新日期：2010-12-01 00:00:00

abstract：:Bacterial secretion systems often employ molecular chaperones to recognize and facilitate export of their substrates. Recent work demonstrated that a secreted component of the type VI secretion system (T6SS), haemolysin co-regulated protein (Hcp), binds directly to effectors, enhancing their stability in the bacterial...

journal_title：Molecular microbiology

authors： Whitney JC,Beck CM,Goo YA,Russell AB,Harding BN,De Leon JA,Cunningham DA,Tran BQ,Low DA,Goodlett DR,Hayes CS,Mougous JD

更新日期：2014-05-01 00:00:00

abstract：:Deletions or substitutions of amino acids at the carboxyl-terminus of the heat-labile enterotoxin B subunit (EtxB) affect its assembly into pentamers in a temperature-dependent manner. At 42 degrees C, the mutations prevent the B subunits from achieving their final pentameric structure resulting in membrane associatio...

journal_title：Molecular microbiology

authors： Sandkvist M,Bagdasarian M

更新日期：1993-11-01 00:00:00

abstract：:Pyruvoyl-dependent histidine decarboxylases are produced as proenzymes that mature by cleavage followed by formation of the pyruvoyl prosthetic group. The histidine decarboxylation pathway of Streptococcus thermophilus CHCC1524 that consists of the pyruvoyl-dependent histidine decarboxylase HdcA and the histidine/hist...

journal_title：Molecular microbiology

authors： Trip H,Mulder NL,Rattray FP,Lolkema JS

更新日期：2011-02-01 00:00:00

abstract：:The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active sta...

journal_title：Molecular microbiology

authors： Nakamura S,Hanaizumi Y,Morimoto YV,Inoue Y,Erhardt M,Minamino T,Namba K

更新日期：2020-04-01 00:00:00

abstract：:Natural sequence variation was investigated among serogroup A subgroup IV-1 Neisseria meningitidis isolated from diseased patients and healthy carriers in The Gambia, West Africa. The frequencies of DNA import were analysed by sequencing fragments of four linked genes encoding the immunogenic outer membrane proteins T...

journal_title：Molecular microbiology

authors： Linz B,Schenker M,Zhu P,Achtman M

更新日期：2000-06-01 00:00:00