Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent.

abstract：:Escherichia coli and Salmonella can use chitin-derived oligosaccharides as carbon and nitrogen sources. Chitosugars traverse the outer membrane through a dedicated chitoporin, ChiP, and are transported across the cytoplasmic membrane by the chitobiose transporter (ChbBCA). Previous work revealed that synthesis of the ...

journal_title：Molecular microbiology

authors： Plumbridge J,Bossi L,Oberto J,Wade JT,Figueroa-Bossi N

更新日期：2014-05-01 00:00:00

abstract：:Yersinia enterocolitica uses type III secretion to transport Yop proteins into the cytoplasm of host cells. Previous work generated hypotheses for both co- and post-translational transport mechanisms in the Yersinia type III pathway. Here, we used ubiquitin (Ub) and UBP1, the Ub-specific protease, to examine whether Y...

journal_title：Molecular microbiology

authors： Quenee LE,Schneewind O

更新日期：2007-07-01 00:00:00

abstract：:Bacterial chemoreceptors cluster into exquisitively sensitive, tunable, highly ordered, polar arrays. While these arrays serve as paradigms of cell signalling in general, it remains unclear what conformational changes transduce signals from the periplasmic tips, where attractants and repellents bind, to the cytoplasmi...

journal_title：Molecular microbiology

authors： Briegel A,Beeby M,Thanbichler M,Jensen GJ

更新日期：2011-11-01 00:00:00

abstract：:Cytochrome c biogenesis describes the posttranslational pathway for the conversion of pre-apocytochrome c into the mature holocytochrome c. It involves an unknown number of consecutive biochemical steps, including translocation of the precursor polypeptide and haem into the periplasm and the covalent linkage between t...

journal_title：Molecular microbiology

authors： Thöny-Meyer L,Ritz D,Hennecke H

更新日期：1994-04-01 00:00:00

abstract：:Biofilm formation in Bacillus subtilis requires the differentiation of a subpopulation of cells responsible for the production of the extracellular matrix that structures the biofilm. Differentiation of matrix-producing cells depends, among other factors, on the FloT and YqfA proteins. These proteins are present exclu...

journal_title：Molecular microbiology

authors： Yepes A,Schneider J,Mielich B,Koch G,García-Betancur JC,Ramamurthi KS,Vlamakis H,López D

更新日期：2012-10-01 00:00:00

abstract：:A key component to the success of Mycobacterium tuberculosis as a pathogen is the ability to sense and adapt metabolically to the diverse range of conditions encountered in vivo, such as oxygen tension, environmental pH and nutrient availability. Although nitrogen is an essential nutrient for every organism, little is...

journal_title：Molecular microbiology

authors： Williams KJ,Jenkins VA,Barton GR,Bryant WA,Krishnan N,Robertson BD

更新日期：2015-09-01 00:00:00

abstract：:The HPr kinase of Gram-positive bacteria is an ATP-dependent serine protein kinase, which phosphorylates the HPr protein of the bacterial phosphotransferase system (PTS) and is involved in the regulation of carbohydrate metabolism. The hprK gene from Enterococcus faecalis was cloned via polymerase chain reaction (PCR)...

journal_title：Molecular microbiology

authors： Kravanja M,Engelmann R,Dossonnet V,Blüggel M,Meyer HE,Frank R,Galinier A,Deutscher J,Schnell N,Hengstenberg W

更新日期：1999-01-01 00:00:00

abstract：:Nitrogen metabolism in Aspergillus nidulans is regulated by AREA, a member of the GATA family of transcription factors. One mechanism that modulates AREA activity involves the rapid degradation of the areA transcript when sufficient NH4+ or Gln are available. This signalling mechanism has been shown to require a regio...

journal_title：Molecular microbiology

authors： Morozov IY,Martinez MG,Jones MG,Caddick MX

更新日期：2000-09-01 00:00:00

abstract：:Protein ubiquitylation plays a major role in the regulation of cellular processes mainly through proteasome-dependent degradation, although it has become increasingly clear that it is also involved in other processes. In the fungus Mucor circinelloides, blue light regulates carotene biosynthesis, with this response co...

journal_title：Molecular microbiology

authors： Silva F,Navarro E,Peñaranda A,Murcia-Flores L,Torres-Martínez S,Garre V

更新日期：2008-11-01 00:00:00

abstract：:Sequence determination of a region downstream from the vanXYc gene in Enterococcus gallinarum BM4174 revealed an open reading frame, designated vanT, that encodes a 698-amino-acid polypeptide with an amino-terminal domain containing 10 predicted transmembrane segments. The protein contained a highly conserved pyridoxa...

journal_title：Molecular microbiology

authors： Arias CA,Martín-Martinez M,Blundell TL,Arthur M,Courvalin P,Reynolds PE

更新日期：1999-03-01 00:00:00

abstract：:In this issue of Molecular Microbiology, Rugarabamu and colleagues investigate the role of rhomboid proteases responsible for adhesin shedding during invasion of the apicomplexan parasite Toxoplasma gondii. This study, together with several other recent publications, raises new questions about the function of these rh...

journal_title：Molecular microbiology

authors： Stewart RJ,Tonkin CJ

更新日期：2015-07-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:Mycoplasma hyopneumoniae induces respiratory disease in swine by colonizing cilia causing ciliostasis, cilial loss and epithelial cell death. Heparin binds to M. hyopneumoniae cells in a dose-dependent manner and blocks its ability to adhere to porcine cilia. We show here that Mhp493 (P216), a paralogue of the cilium ...

journal_title：Molecular microbiology

authors： Wilton J,Jenkins C,Cordwell SJ,Falconer L,Minion FC,Oneal DC,Djordjevic MA,Connolly A,Barchia I,Walker MJ,Djordjevic SP

更新日期：2009-02-01 00:00:00

abstract：:Quorum-sensing control of luminescence in Vibrio harveyi, which involves an indirect autoinducer-mediated phosphorelay signal transduction system, contrasts with the prototypical quorum-sensing system of Vibrio fischeri, in which the autoinducer and the transcriptional activator LuxR directly activate lux operon expre...

journal_title：Molecular microbiology

authors： Miyamoto CM,Dunlap PV,Ruby EG,Meighen EA

更新日期：2003-04-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that is notorious for its intrinsic drug resistance. We have used chemical and genetic techniques to characterize three putative kinase genes that are involved in the addition of phosphate to the inner core region of P. aeruginosa lipopolysaccharide. The first gene i...

journal_title：Molecular microbiology

authors： Walsh AG,Matewish MJ,Burrows LL,Monteiro MA,Perry MB,Lam JS

更新日期：2000-02-01 00:00:00

abstract：:Type IV secretion systems are virulence determinants in many bacteria and share extensive homology with many conjugal transfer systems. Although type IV systems and their homologues have been studied widely, the mechanism by which substrates are secreted remains unclear. In Agrobacterium, we show that type IV secretio...

journal_title：Molecular microbiology

authors： Pantoja M,Chen L,Chen Y,Nester EW

更新日期：2002-09-01 00:00:00

abstract：:srfA is a locus required for the production of the lipopeptide antibiotic surfactin. This locus is also necessary for efficient sporulation and competence development. Mutations in the 5' portion of the srfA operon affect all three of these processes, whereas mutations in the 3' portion of srfA only affect sporulation...

journal_title：Molecular microbiology

authors： van Sinderen D,Galli G,Cosmina P,de Ferra F,Withoff S,Venema G,Grandi G

更新日期：1993-05-01 00:00:00

abstract：:Bacterial spores are surrounded by a multilayered proteinaceous shell called the coat. In Bacillus subtilis, a coat protein called CotE guides the assembly of a major subset of coat proteins. To understand how CotE carries out its role in coat morphogenesis, we subjected its gene to mutagenesis and studied the effects...

journal_title：Molecular microbiology

authors： Little S,Driks A

更新日期：2001-11-01 00:00:00

abstract：:The crystal structures of two active forms of dissimilatory sulphite reductase (Dsr) from Desulfovibrio gigas, Dsr-I and Dsr-II, are compared at 1.76 and 2.05 Å resolution respectively. The dimeric α2β2γ2 structure of Dsr-I contains eight [4Fe-4S] clusters, two saddle-shaped sirohaems and two flat sirohydrochlorins. I...

journal_title：Molecular microbiology

authors： Hsieh YC,Liu MY,Wang VC,Chiang YL,Liu EH,Wu WG,Chan SI,Chen CJ

更新日期：2010-12-01 00:00:00

abstract：:Folate metabolism in malaria parasites is a long-standing, clinical target for chemotherapy and prophylaxis. However, despite determination of the complete genome sequence of the lethal species Plasmodium falciparum, the pathway of de novo folate biosynthesis remains incomplete, as no candidate gene for dihydroneopter...

journal_title：Molecular microbiology

authors： Dittrich S,Mitchell SL,Blagborough AM,Wang Q,Wang P,Sims PF,Hyde JE

更新日期：2008-02-01 00:00:00

abstract：:Retinal and its derivatives represent essential compounds in many biological systems. In animals, they are synthesized through a symmetrical cleavage of beta-carotene catalysed by a monooxygenase. Here, we demonstrate that the open reading frame sll1541 from the cyanobacterium Synechocystis sp. PCC 6803 encodes the fi...

journal_title：Molecular microbiology

authors： Ruch S,Beyer P,Ernst H,Al-Babili S

更新日期：2005-02-01 00:00:00

abstract：:Metal ions are crucial trace elements for bacteria infecting the human host. The LraI (lipoprotein receptor-associated antigen I) transporter in Streptococcus spp. belongs to the superfamily of ABC transporters. The transporter consists of a lipoprotein, an ATP-binding protein and a hydrophobic integral membrane prote...

journal_title：Molecular microbiology

authors： Janulczyk R,Pallon J,Björck L

更新日期：1999-11-01 00:00:00

abstract：:Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

journal_title：Molecular microbiology

authors： Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

更新日期：2005-06-01 00:00:00

abstract：:An msDNA operon, consisting of genes for msDNA and a reverse transcriptase, is present in Escherichia coliB and absent from E. coliK12. We have found that the msDNA operon is located on a DNA fragment, longer than 15kb, that is absent from E. coliK12. Using conjugation, P1 transduction, and nucleic acid hybridization ...

journal_title：Molecular microbiology

authors： Lim D,Maas WK

更新日期：1990-12-01 00:00:00

abstract：:The SaPIs are chromosomal islands in staphylococci and other Gram-positive bacteria that carry genes for superantigens, virulence factors, resistance and certain metabolic functions. They have intimate relationships with certain temperate phages involving phage-induced excision, replication and efficient packaging in ...

journal_title：Molecular microbiology

authors： Ubeda C,Maiques E,Barry P,Matthews A,Tormo MA,Lasa I,Novick RP,Penadés JR

更新日期：2008-02-01 00:00:00

abstract：:The AMA1 sequence is an efficient plasmid replicator and transformation enhancer in Aspergillus nidulans. It comprises two long perfect inverted repeats (MATE elements) flanking a short, unique, central spacer. Subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is ne...

journal_title：Molecular microbiology

authors： Aleksenko A,Gems D,Clutterbuck J

更新日期：1996-04-01 00:00:00

abstract：:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medi...

journal_title：Molecular microbiology

authors： Repoila F,Gutierrez C

更新日期：1991-03-01 00:00:00

abstract：:Random gene tagging was used to obtain new mutants of the marine cyanobacterium, Synechococcus sp. PCC7002, with defects in the CO2-concentrating mechanism (CCM). Two of these mutants, K22 and A41, showed poor growth at limiting CO2. Isolation and sequencing of a 6. 6 kb genomic region revealed the existence of five p...

journal_title：Molecular microbiology

authors： Klughammer B,Sültemeyer D,Badger MR,Price GD

更新日期：1999-06-01 00:00:00

abstract：:Pseudomonas syringae pv. syringae cell densities fluctuate regularly during host plant colonization. Previously we identified nine genes dependent on the quorum-sensing-associated luxR homolog ahlR during epiphytic and apoplastic stages of host colonization. Yet their contributions to host colonization remain obscure,...

journal_title：Molecular microbiology

authors： Scott RA,Lindow SE

更新日期：2016-03-01 00:00:00

abstract：:CTXphi is a filamentous phage that encodes cholera toxin, one of the principal virulence factors of Vibrio cholerae. CTXphi is unusual among filamentous phages because it can either replicate as a plasmid or integrate into the V. cholerae chromosome at a specific site. The CTXphi genome has two regions, the 'core' and...

journal_title：Molecular microbiology

authors： Waldor MK,Rubin EJ,Pearson GD,Kimsey H,Mekalanos JJ

更新日期：1997-06-01 00:00:00