Retinal biosynthesis in Eubacteria: in vitro characterization of a novel carotenoid oxygenase from Synechocystis sp. PCC 6803.


:Retinal and its derivatives represent essential compounds in many biological systems. In animals, they are synthesized through a symmetrical cleavage of beta-carotene catalysed by a monooxygenase. Here, we demonstrate that the open reading frame sll1541 from the cyanobacterium Synechocystis sp. PCC 6803 encodes the first eubacterial, retinal synthesizing enzyme (Diox1) thus far reported. In contrast to enzymes from animals, Diox1 converts beta-apo-carotenals instead of beta-carotene into retinal in vitro. The identity of the enzymatic product was proven by HPLC, GC-MS and in a biological test. Investigations, of the stereospecifity showed that Diox1 cleaved only the all-trans form of beta-apo-8'-carotenal, yielding all-trans-retinal. However, Diox1 exhibited wide substrate specificity with respect to chain-lengths and functional end-groups. Although with divergent Km and Vmax values, the enzyme converted beta-apo-carotenals, (3R)-3-OH-beta-apo-carotenals as well as apo-lycopenals into retinal, (3R)-3-hydroxy-retinal and acycloretinal respectively. In addition, the alcohols of these substrates were cleaved to yield the corresponding retinal derivatives.


Mol Microbiol


Molecular microbiology


Ruch S,Beyer P,Ernst H,Al-Babili S




Has Abstract


2005-02-01 00:00:00














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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

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    journal_title:Molecular microbiology

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    authors: Ding Q,Kusano S,Villarejo M,Ishihama A

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  • The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase.

    abstract::Salmonella translocate a group of type III effectors into the host cells to induce entry, promote survival and cause intestinal inflammation. Although the biochemical and cellular mechanisms of how bacterial effectors function inside host cells remain largely unknown, studies have indicated that a likely strategy is t...

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    authors: Zhang Y,Higashide WM,McCormick BA,Chen J,Zhou D

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    authors: Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

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    journal_title:Molecular microbiology

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    authors: Economou A,Hawkins FK,Downie JA,Johnston AW

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    authors: Barfod L,Bernasconi NL,Dahlbäck M,Jarrossay D,Andersen PH,Salanti A,Ofori MF,Turner L,Resende M,Nielsen MA,Theander TG,Sallusto F,Lanzavecchia A,Hviid L

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    authors: Xu W,Shen J,Dunn CA,Desai S,Bessman MJ

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    journal_title:Molecular microbiology

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    journal_title:Molecular microbiology

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kao R,Shea JE,Davies J,Holden DW

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Handa N,Nakayama Y,Sadykov M,Kobayashi I

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nowicki EM,O'Brien JP,Brodbelt JS,Trent MS

    更新日期:2015-07-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Quinn FD,Tompkins LS

    更新日期:1989-06-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Duong F,Lazdunski A,Murgier M

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    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Bustamante VH,Calva E

    更新日期:2014-03-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ogura M,Shimane K,Asai K,Ogasawara N,Tanaka T

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    pub_type: 杂志文章,评审


    authors: Sumrall ET,Keller AP,Shen Y,Loessner MJ

    更新日期:2020-03-01 00:00:00