Retinal biosynthesis in Eubacteria: in vitro characterization of a novel carotenoid oxygenase from Synechocystis sp. PCC 6803.

Abstract:

:Retinal and its derivatives represent essential compounds in many biological systems. In animals, they are synthesized through a symmetrical cleavage of beta-carotene catalysed by a monooxygenase. Here, we demonstrate that the open reading frame sll1541 from the cyanobacterium Synechocystis sp. PCC 6803 encodes the first eubacterial, retinal synthesizing enzyme (Diox1) thus far reported. In contrast to enzymes from animals, Diox1 converts beta-apo-carotenals instead of beta-carotene into retinal in vitro. The identity of the enzymatic product was proven by HPLC, GC-MS and in a biological test. Investigations, of the stereospecifity showed that Diox1 cleaved only the all-trans form of beta-apo-8'-carotenal, yielding all-trans-retinal. However, Diox1 exhibited wide substrate specificity with respect to chain-lengths and functional end-groups. Although with divergent Km and Vmax values, the enzyme converted beta-apo-carotenals, (3R)-3-OH-beta-apo-carotenals as well as apo-lycopenals into retinal, (3R)-3-hydroxy-retinal and acycloretinal respectively. In addition, the alcohols of these substrates were cleaved to yield the corresponding retinal derivatives.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Ruch S,Beyer P,Ernst H,Al-Babili S

doi

10.1111/j.1365-2958.2004.04460.x

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

1015-24

issue

4

eissn

0950-382X

issn

1365-2958

pii

MMI4460

journal_volume

55

pub_type

杂志文章
  • In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.

    abstract::DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04408.x

    authors: Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

    更新日期:2005-01-01 00:00:00

  • Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes.

    abstract::Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1995.tb02427.x

    authors: Ding Q,Kusano S,Villarejo M,Ishihama A

    更新日期:1995-05-01 00:00:00

  • The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase.

    abstract::Salmonella translocate a group of type III effectors into the host cells to induce entry, promote survival and cause intestinal inflammation. Although the biochemical and cellular mechanisms of how bacterial effectors function inside host cells remain largely unknown, studies have indicated that a likely strategy is t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05407.x

    authors: Zhang Y,Higashide WM,McCormick BA,Chen J,Zhou D

    更新日期:2006-11-01 00:00:00

  • Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs.

    abstract::Quinone-based compounds have been exploited to treat infectious diseases and cancer, with such chemicals often functioning as inhibitors of key metabolic pathways or as prodrugs. Here, we screened an aziridinyl 1,4-benzoquinone (ABQ) library against the causative agents of trypanosomiasis, and cutaneous leishmaniasis,...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13767

    authors: Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

    更新日期:2017-10-01 00:00:00

  • Calcineurin responsive zinc-finger-1 binds to a unique promoter sequence to upregulate neuronal calcium sensor-1, whose interaction with MID-1 increases tolerance to calcium stress in Neurospora crassa.

    abstract::We studied the molecular mechanism of neuronal calcium sensor-1 (NCS-1) signaling pathway for tolerance to Ca2+ stress in Neurospora crassa. Increasing concentration of Ca2+ increased the expression of ncs-1; however, the calcineurin inhibitor FK506 severely reduced ncs-1 mRNA transcript levels. Chromatin immunoprecip...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14234

    authors: Gohain D,Tamuli R

    更新日期:2019-06-01 00:00:00

  • Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.

    abstract::Natural competence in Streptococcus pneumoniae is regulated by a quorum-sensing mechanism consisting of a competence-stimulating peptide (CSP), its dedicated secretion apparatus (ComAB), its histidine kinase receptor (ComD) and a response regulator (ComE). In this report, we show that ComE is a DNA-binding protein tha...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1999.01528.x

    authors: Ween O,Gaustad P,Håvarstein LS

    更新日期:1999-08-01 00:00:00

  • Multiple copies of a DNA sequence from Pseudomonas syringae pathovar phaseolicola abolish thermoregulation of phaseolotoxin production.

    abstract::Phaseolotoxin, a phytotoxin of Pseudomonas syringae pv. phaseolicola, is produced at 18 degrees C but not at 28 degrees C. Here we report that a fragment (24.4 kb) cloned from the wild-type strain, which does not harbour a gene(s) involved in phaseolotoxin biosynthesis, abolishes this thermoregulation in the wild type...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01606.x

    authors: Rowley KB,Clements DE,Mandel M,Humphreys T,Patil SS

    更新日期:1993-05-01 00:00:00

  • One if by land, two if by sea: signalling to the ranks with CSP and XIP.

    abstract::In many streptococci, quorum sensing utilizes secreted, linear peptides that engage cognate receptors to coordinate gene expression among members of a local population. Streptococcus mutans employs the secreted peptides CSP and XIP to stimulate production of antimicrobial bacteriocins and to induce development of comp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12029

    authors: Federle MJ,Morrison DA

    更新日期:2012-10-01 00:00:00

  • Molecular population genetic analysis of the enn subdivision of group A streptococcal emm-like genes: horizontal gene transfer and restricted variation among enn genes.

    abstract::The group A streptococcal emm-like genes, which encode the cell-surface M and M-like proteins, are divided into distinct mrp, emm and enn subdivisions and are clustered together in a region of the chromosome called the vir regulon. In order to understand the mechanisms involved in the evolution of emm-like genes, a 18...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1995.tb02279.x

    authors: Whatmore AM,Kapur V,Musser JM,Kehoe MA

    更新日期:1995-03-01 00:00:00

  • Transcription of rhiA, a gene on a Rhizobium leguminosarum bv. viciae Sym plasmid, requires rhiR and is repressed by flavanoids that induce nod genes.

    abstract::Strains of Rhizobium leguminosarum biovar viciae specifically make an abundant protein (Rhi) in free-living culture but not in bacteroids. Genes needed for Rhi synthesis are on a Sym plasmid and here we show that one of these genes, rhiA, is the structural gene that specifies this polypeptide. Transcription of rhiA re...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1989.tb00107.x

    authors: Economou A,Hawkins FK,Downie JA,Johnston AW

    更新日期:1989-01-01 00:00:00

  • Human pregnancy-associated malaria-specific B cells target polymorphic, conformational epitopes in VAR2CSA.

    abstract::Pregnancy-associated malaria (PAM) is caused by Plasmodium falciparum-infected erythrocytes (IEs) that bind to chondroitin sulphate A (CSA) in the placenta by PAM-associated clonally variant surface antigens (VSA). Pregnancy-specific VSA (VSA(PAM)), which include the PfEMP1 variant VAR2CSA, are targets of IgG-mediated...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05503.x

    authors: Barfod L,Bernasconi NL,Dahlbäck M,Jarrossay D,Andersen PH,Salanti A,Ofori MF,Turner L,Resende M,Nielsen MA,Theander TG,Sallusto F,Lanzavecchia A,Hviid L

    更新日期:2007-01-01 00:00:00

  • Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants.

    abstract::Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03595.x

    authors: Andrews KT,Pirrit LA,Przyborski JM,Sanchez CP,Sterkers Y,Ricken S,Wickert H,Lépolard C,Avril M,Scherf A,Gysin J,Lanzer M

    更新日期:2003-08-01 00:00:00

  • The rpsL gene and streptomycin resistance in single and multiple drug-resistant strains of Mycobacterium tuberculosis.

    abstract::The recent emergence of indolent and rapidly fatal drug-resistant strains of Mycobacterium tuberculosis has renewed interest in defining the molecular mechanisms of drug resistance in the tubercle bacilli. In this report, we have examined the mechanism of resistance to streptomycin (Sm) in M. tuberculosis through the ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb00924.x

    authors: Nair J,Rouse DA,Bai GH,Morris SL

    更新日期:1993-11-01 00:00:00

  • Two essential FtsH proteases control the level of the Fur repressor during iron deficiency in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::The cyanobacterium Synechocystis sp. PCC 6803 expresses four different FtsH protease subunits (FtsH1-4) that assemble into specific homo- and heterocomplexes. The FtsH2/FtsH3 complex is involved in photoprotection but the physiological roles of the other complexes, notably the essential FtsH1/FtsH3 complex, remain unc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12782

    authors: Krynická V,Tichý M,Krafl J,Yu J,Kaňa R,Boehm M,Nixon PJ,Komenda J

    更新日期:2014-11-01 00:00:00

  • A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus.

    abstract::Oxidative stress serves as an important host/environmental signal that triggers a wide range of responses from the human pathogen Staphylococcus aureus. Among these, a thiol-based oxidation sensing pathway through a global regulator MgrA controls the virulence and antibiotic resistance of the bacterium. Herein, we rep...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06518.x

    authors: Chen PR,Nishida S,Poor CB,Cheng A,Bae T,Kuechenmeister L,Dunman PM,Missiakas D,He C

    更新日期:2009-01-01 00:00:00

  • Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.

    abstract::mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.00821.x

    authors: Moe PC,Blount P,Kung C

    更新日期:1998-05-01 00:00:00

  • The Nudix hydrolases of Deinococcus radiodurans.

    abstract::All 21 of the Nudix hydrolase genes from the radiation-resistant organism Deinococcus radiodurans have been cloned into vectors under the control of T7 promoters and expressed as soluble proteins in Escherichia coli. Their sizes range from 9.8 kDa (91 amino acids) to 59 kDa (548 amino acids). Two novel proteins were i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02267.x

    authors: Xu W,Shen J,Dunn CA,Desai S,Bessman MJ

    更新日期:2001-01-01 00:00:00

  • Plant defence suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae.

    abstract::Crop destruction by the hemibiotrophic rice pathogen Magnaporthe oryzae requires plant defence suppression to facilitate extensive biotrophic growth in host cells before the onset of necrosis. How this is achieved at the genetic level is not well understood. Here, we report that a M. oryzae sirtuin, MoSir2, plays an e...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12743

    authors: Fernandez J,Marroquin-Guzman M,Nandakumar R,Shijo S,Cornwell KM,Li G,Wilson RA

    更新日期:2014-10-01 00:00:00

  • Global regulatory roles of the cAMP/PKA pathway revealed by phenotypic, transcriptomic and phosphoproteomic analyses in a null mutant of the PKA catalytic subunit in Candida albicans.

    abstract::The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence in the human fungal pathogen Candida albicans. It has long been thought that the PKA catalytic subunit is essential for cell viability in this fungus. Paradoxically, the single adenylyl c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13681

    authors: Cao C,Wu M,Bing J,Tao L,Ding X,Liu X,Huang G

    更新日期:2017-07-01 00:00:00

  • Identification of novel hrp-regulated genes through functional genomic analysis of the Pseudomonas syringae pv. tomato DC3000 genome.

    abstract::Pseudomonas syringae pv. tomato (Pst) strain DC3000 infects the model plants Arabidopsis thaliana and tomato, causing disease symptoms characterized by necrotic lesions surrounded by chlorosis. One mechanism used by Pst DC3000 to infect host plants is the type III protein secretion system, which is thought to deliver ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2002.02964.x

    authors: Zwiesler-Vollick J,Plovanich-Jones AE,Nomura K,Bandyopadhyay S,Joardar V,Kunkel BN,He SY

    更新日期:2002-09-01 00:00:00

  • Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.

    abstract::Bacterial spores are surrounded by a multilayered proteinaceous shell called the coat. In Bacillus subtilis, a coat protein called CotE guides the assembly of a major subset of coat proteins. To understand how CotE carries out its role in coat morphogenesis, we subjected its gene to mutagenesis and studied the effects...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02708.x

    authors: Little S,Driks A

    更新日期:2001-11-01 00:00:00

  • Probing the active site of mitogillin, a fungal ribotoxin.

    abstract::Fungal ribotoxins, such as mitogillin and the related Aspergillus toxins restrictocin and alpha-sarcin, are highly specific ribonucleases, which inactivate the ribosome enzymatically by cleaving the eukaryotic 28S RNA of the large ribosomal subunit at a single phosphodiester bond. The site of cleavage occurs between G...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.00987.x

    authors: Kao R,Shea JE,Davies J,Holden DW

    更新日期:1998-08-01 00:00:00

  • Experimental genome evolution: large-scale genome rearrangements associated with resistance to replacement of a chromosomal restriction-modification gene complex.

    abstract::Type II restriction enzymes are paired with modification enzymes that protect type II restriction sites from cleavage by methylating them. A plasmid carrying a type II restriction-modification gene complex is not easily replaced by an incompatible plasmid because loss of the former leads to cell death through chromoso...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02436.x

    authors: Handa N,Nakayama Y,Sadykov M,Kobayashi I

    更新日期:2001-05-01 00:00:00

  • Extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system.

    abstract::Gram-negative bacteria survive harmful environmental stressors by modifying their outer membrane. Much of this protection is afforded upon remodeling of the lipid A region of the major surface molecule lipopolysaccharide (LPS). For example, the addition of cationic substituents, such as 4-amino-4-deoxy-L-arabinose (L-...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13018

    authors: Nowicki EM,O'Brien JP,Brodbelt JS,Trent MS

    更新日期:2015-07-01 00:00:00

  • Streptococcus mutans autolysin AtlA is a fibronectin-binding protein and contributes to bacterial survival in the bloodstream and virulence for infective endocarditis.

    abstract::Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2009.06903.x

    authors: Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

    更新日期:2009-11-01 00:00:00

  • Analysis of a cloned sequence of Legionella pneumophila encoding a 38 kD metalloprotease possessing haemolytic and cytotoxic activities.

    abstract::The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1989.tb00228.x

    authors: Quinn FD,Tompkins LS

    更新日期:1989-06-01 00:00:00

  • Protein secretion by heterologous bacterial ABC-transporters: the C-terminus secretion signal of the secreted protein confers high recognition specificity.

    abstract::Pseudomonas aeruginosa releases several extracellular proteins which are secreted via two independent secretion pathways. Alkaline protease (AprA) Is released by its own specific secretion machinery which is an ABC-transporter. Despite sequence similarities between components of ABC-transporters in different bacteria,...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1996.tb02555.x

    authors: Duong F,Lazdunski A,Murgier M

    更新日期:1996-08-01 00:00:00

  • LeuO, a dormant sentinel for SPI-1?

    abstract::A new mechanism for the turning-off of gene expression in Salmonella Pathogenicity Island 1 (SPI-1) is proposed by Espinosa and Casadesús, which involves the action of the LeuO quiescent regulator, by two different pathways. A major one through the activation of the hilE gene, where the HilE protein would in turn inac...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章

    doi:10.1111/mmi.12514

    authors: Bustamante VH,Calva E

    更新日期:2014-03-01 00:00:00

  • Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis.

    abstract::We screened the putative rap-phr (response regulator aspartyl-phosphate phosphatase-phosphatase regulator) systems identified in the Bacillus subtilis genome for a rap gene that affects aprE (alkaline protease gene) expression by using a multicopy plasmid. We found that rapG was involved in the regulation of aprE, whi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03665.x

    authors: Ogura M,Shimane K,Asai K,Ogasawara N,Tanaka T

    更新日期:2003-09-01 00:00:00

  • Structure and function of Listeria teichoic acids and their implications.

    abstract::Teichoic acids (TAs) are the most abundant glycopolymers in the cell wall of Listeria, an opportunistic Gram-positive pathogen that causes severe foodborne infections. Two different structural classes of Listeria TA exist: the polyribitolphosphate-based wall teichoic acid (WTA) that is covalently anchored to the pepti...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.14472

    authors: Sumrall ET,Keller AP,Shen Y,Loessner MJ

    更新日期:2020-03-01 00:00:00