Abstract:
:We have characterized a series of amber mutations in the A gene of bacteriophage Mu encoding the phage transposase. We tested different activities of these mutant proteins either in a sup0 strain or in different sup bacteria. In conjunction with the results described in the accompanying paper by Bétermier et al. (1989) we find that the C-terminus of the protein is not absolutely essential for global transposase function, but is essential for phage growth. Specific binding to Mu ends is defined by a more central domain. Our results also reinforce the previous findings (Bétermier et al., 1987) that more than one protein may be specified by the A gene.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Desmet L,Faelen M,Gama MJ,Ferhat A,Toussaint Adoi
10.1111/j.1365-2958.1989.tb00265.xsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
1145-58issue
9eissn
0950-382Xissn
1365-2958journal_volume
3pub_type
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