Escherichia coli nusG mutations that block transcription termination by coliphage HK022 Nun protein.

abstract：:The availability of nutrients is a major determinant for the timing of morphogenesis and antibiotic production in the soil-dwelling bacterium Streptomyces coelicolor. Here we show that N-acetylglucosamine transport, the first step of an important nutrient signalling cascade, is mediated by the NagE2 permease of the ph...

journal_title：Molecular microbiology

authors： Nothaft H,Rigali S,Boomsma B,Swiatek M,McDowall KJ,van Wezel GP,Titgemeyer F

更新日期：2010-03-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:Ureaplasma urealyticum (U. urealyticum), belonging to the class Mollicutes, is a human pathogen colonizing the urogenital tract and causes among other things respiratory diseases in premature infants. We have studied the salvage of pyrimidine deoxynucleosides in U. urealyticum and cloned a key salvage enzyme, thymidin...

journal_title：Molecular microbiology

authors： Carnrot C,Wehelie R,Eriksson S,Bölske G,Wang L

更新日期：2003-11-01 00:00:00

abstract：:RepA, the initiator protein of plasmid P1, binds to multiple sites (iterons) in the origin. The binding normally requires participation of chaperones, DnaJ, DnaK and GrpE. When purified, RepA appears dimeric and is inactive in iteron binding. On reaction with chaperones, a species active in iteron binding is formed an...

journal_title：Molecular microbiology

authors： Dibbens JA,Muraiso K,Chattoraj DK

更新日期：1997-10-01 00:00:00

abstract：:Previous work demonstrated that selection for Escherichia coli mutants with low antibiotic resistance frequently resulted in co-selection of lon mutations and that lon(-) mutants evolved higher-level resistance faster than a lon(+) strain. Here we show that lon mutation causes a very low multidrug resistance by induci...

journal_title：Molecular microbiology

authors： Nicoloff H,Andersson DI

更新日期：2013-12-01 00:00:00

abstract：:Leishmania parasites target macrophages in their mammalian hosts and proliferate within the mature phagolysosome compartment of these cells. Intracellular amastigote stages are dependent on sugars as a major carbon source in vivo, but retain the capacity to utilize other carbon sources. To investigate whether amastigo...

journal_title：Molecular microbiology

authors： Saunders EC,Naderer T,Chambers J,Landfear SM,McConville MJ

更新日期：2018-04-01 00:00:00

abstract：:The mechanisms of transcription repression and derepression in vivo are not fully understood. We have obtained evidence that begins to clarify the minimum requirements for counteracting nucleosomal repression in vivo. Location of the TATA element near the nucleosome dyad does not block RNA polymerase II transcription ...

journal_title：Molecular microbiology

authors： Moss DR,Laybourn PJ

更新日期：2000-06-01 00:00:00

abstract：:Bacteria utilize phosphorelay systems to respond to environmental or intracellular stimuli. Salmonella enterica encodes a four-step phosphorelay system that involves two sensor kinase proteins, RcsC and RcsD, and a response regulator, RcsB. The physiological stimulus for Rcs phosphorelay activation is unknown; however...

journal_title：Molecular microbiology

authors： Erickson KD,Detweiler CS

更新日期：2006-11-01 00:00:00

abstract：:Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...

journal_title：Molecular microbiology

authors： Grainger DC,Goldberg MD,Lee DJ,Busby SJ

更新日期：2008-06-01 00:00:00

abstract：:Spatio-temporal oscillations of the Min proteins are essential for selecting the cell division site in Escherichia coli. These oscillations are a key example of a biological phenomenon that can only be understood on a systems level rather than on the level of its individual components. Here, we review the key concepts...

journal_title：Molecular microbiology

authors： Kruse K,Howard M,Margolin W

更新日期：2007-03-01 00:00:00

abstract：:The Staphylococcus epidermidis genes icaABC are involved in the synthesis of the polysaccharide intercellular adhesin (PIA), which is located mainly on the cell surface, as shown by immunofluorescence studies with PIA-specific antiserum. PIA was shown to be a linear beta-1,6-linked glucosaminoglycan composed of at lea...

journal_title：Molecular microbiology

authors： Heilmann C,Schweitzer O,Gerke C,Vanittanakom N,Mack D,Götz F

更新日期：1996-06-01 00:00:00

abstract：:In the past, ultrastructural investigations of Leishmania mexicana amastigotes revealed structures that were tentatively identified as autophagosomes. This study has now provided definitive data that autophagy occurs in the parasite during differentiation both to metacyclic promastigotes and to amastigotes, autophagos...

journal_title：Molecular microbiology

authors： Williams RA,Tetley L,Mottram JC,Coombs GH

更新日期：2006-08-01 00:00:00

abstract：:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (sigma) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cau...

journal_title：Molecular microbiology

authors： Manganelli R,Voskuil MI,Schoolnik GK,Dubnau E,Gomez M,Smith I

更新日期：2002-07-01 00:00:00

abstract：:We used the LacO/GFP-LacI system to label and visualize the IncP beta plasmid R751 fluorescently during conjugative transfer between live donor and recipient bacteria. Comparisons of R751 in conjugative and non-conjugative conditions have allowed us to identify key localizations and movements associated with the initi...

journal_title：Molecular microbiology

authors： Lawley TD,Gordon GS,Wright A,Taylor DE

更新日期：2002-05-01 00:00:00

abstract：:Quinone-based compounds have been exploited to treat infectious diseases and cancer, with such chemicals often functioning as inhibitors of key metabolic pathways or as prodrugs. Here, we screened an aziridinyl 1,4-benzoquinone (ABQ) library against the causative agents of trypanosomiasis, and cutaneous leishmaniasis,...

journal_title：Molecular microbiology

authors： Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

更新日期：2017-10-01 00:00:00

abstract：:The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green f...

journal_title：Molecular microbiology

authors： Bryan SJ,Burroughs NJ,Shevela D,Yu J,Rupprecht E,Liu LN,Mastroianni G,Xue Q,Llorente-Garcia I,Leake MC,Eichacker LA,Schneider D,Nixon PJ,Mullineaux CW

更新日期：2014-10-13 00:00:00

abstract：:The hok/sok locus of plasmid R1 mediates plasmid stabilization by the killing of plasmid-free cells. Many bacterial plasmids carry similar loci. For example, the F plasmid carries two hok homologues, flm and srnB, that mediate plasmid stabilization by this specialized type of programmed cell death. Here, we show that ...

journal_title：Molecular microbiology

authors： Pedersen K,Gerdes K

更新日期：1999-06-01 00:00:00

abstract：:Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...

journal_title：Molecular microbiology

authors： Peterson SN,Sung CK,Cline R,Desai BV,Snesrud EC,Luo P,Walling J,Li H,Mintz M,Tsegaye G,Burr PC,Do Y,Ahn S,Gilbert J,Fleischmann RD,Morrison DA

更新日期：2004-02-01 00:00:00

abstract：:Toxin-antitoxin (TA) systems on the chromosomes of free-living bacteria appear to facilitate cell survival during intervals of stress by inducing a state of reversible growth arrest. However, upon prolonged stress, TA toxin action leads to cell death. They have been implicated in several clinically important phenomena...

journal_title：Molecular microbiology

authors： Prysak MH,Mozdzierz CJ,Cook AM,Zhu L,Zhang Y,Inouye M,Woychik NA

更新日期：2009-03-01 00:00:00

abstract：:GPI-anchoring is a universal and critical post-translational protein modification in eukaryotes. In fungi, many cell wall proteins are GPI-anchored, and disruption of GPI-anchored proteins impairs cell wall integrity. After being synthesized and attached to target proteins, GPI anchors undergo modification on lipid mo...

journal_title：Molecular microbiology

authors： Chung D,Thammahong A,Shepardson KM,Blosser SJ,Cramer RA

更新日期：2014-06-01 00:00:00

abstract：:The CovR/S two-component system regulates the transcription of many genes that are crucial for the virulence of Streptococcus pyogenes (group A Streptococcus, GAS). Previously, we demonstrated that one gene repressed directly by CovR is rivR, which encodes a member of the RofA-like family of transcriptional regulators...

journal_title：Molecular microbiology

authors： Roberts SA,Scott JR

更新日期：2007-12-01 00:00:00

abstract：:In cyanobacteria and chloroplasts, exposure to HL damages the photosynthetic apparatus, especially the D1 subunit of Photosystem II. To avoid chronic photoinhibition, a PSII repair cycle operates to replace damaged PSII subunits with newly synthesised versions. To determine the sub-cellular location of this process, w...

journal_title：Molecular microbiology

authors： Sacharz J,Bryan SJ,Yu J,Burroughs NJ,Spence EM,Nixon PJ,Mullineaux CW

更新日期：2015-05-01 00:00:00

abstract：:Genes controlling chemotaxis towards L-amino acids and D-mannitol in Rhizobium meliloti have been identified by Tn5 insertions that lead to chemotaxis-deficient mutants. The tagged genes span an 8.7 kbp region that has been sequenced. These genes are part of a large operon containing three novel open reading frames, o...

journal_title：Molecular microbiology

authors： Greck M,Platzer J,Sourjik V,Schmitt R

更新日期：1995-03-01 00:00:00

abstract：:A region of 7.8 kb of the plasmid pMB2 from Enterococcus faecalis S-48 carrying the information necessary for production and immunity of the peptide antibiotic AS-48 has been cloned and sequenced. It contains the as-48A structural gene plus five open reading frames (as-48B, as-48C, as-48C1, as-48D and as-48D1). Beside...

journal_title：Molecular microbiology

authors： Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

更新日期：1998-01-01 00:00:00

abstract：:citS and citT genes encoding a new two-component system were identified in the 71 degrees region between the pel and citM loci on the Bacillus subtilis chromosome. citS- and citT-deficient strains were unable to grow on minimal plates including citrate as a sole carbon source. In addition, a strain deficient in citM, ...

journal_title：Molecular microbiology

authors： Yamamoto H,Murata M,Sekiguchi J

更新日期：2000-08-01 00:00:00

abstract：:ToxR of Vibrio cholerae directly activates the ompU promoter, but requires a second activator, TcpP to activate the toxT promoter. ompU encodes a porin, while toxT encodes the transcription factor, ToxT, which activates V. cholerae virulence genes including cholera toxin and the toxin co-regulated pilus. Using an ompU...

journal_title：Molecular microbiology

authors： Morgan SJ,Felek S,Gadwal S,Koropatkin NM,Perry JW,Bryson AB,Krukonis ES

更新日期：2011-07-01 00:00:00

abstract：:The Escherichia coli DNA glycosylase Mug excises 3,N(4)-ethenocytosines (epsilon C) and uracils from DNA, but its biological function is obscure. This is because epsilon C is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We ...

journal_title：Molecular microbiology

authors： Mokkapati SK,Fernández de Henestrosa AR,Bhagwat AS

更新日期：2001-09-01 00:00:00

abstract：:Regions close to the replication terminus of the Escherichia coli chromosome are strongly refractory to genomic inversions. Since these regions also harbour polar replication terminator-like sequences or pause sites, we have investigated the possibility that slowing of replication as a result of pausing at inverted pa...

journal_title：Molecular microbiology

authors： François V,Louarn J,Patte J,Rebollo JE,Louarn JM

更新日期：1990-04-01 00:00:00

abstract：:Interactions between domains of ATP-binding cassette (ABC) transporters are of great functional importance and yet are poorly understood. To gain further knowledge of these protein-protein interactions, we studied the inner membrane complex of the maltose transporter of Escherichia coli. We focused on interactions bet...

journal_title：Molecular microbiology

authors： Mourez M,Jéhanno M,Schneider E,Dassa E

更新日期：1998-10-01 00:00:00

abstract：:The cell wall of the unicellular green alga Chlamydomonas reinhardtii exclusively consists of hydroxyproline-containing glycoproteins. Protein chemical analysis of its polypeptide constituents was hindered by their cross-linking via peroxidase-catalysed intermolecular isodityrosine formation and transaminase-dependent...

journal_title：Molecular microbiology

authors： Voigt J,Kiess M,Getzlaff R,Wöstemeyer J,Frank R

更新日期：2010-09-01 00:00:00