Generation of the heterodimeric precursor GP3 of the Chlamydomonas cell wall.

abstract：:9 alpha-Hydroxylation of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial steroid catabolism. Very limited knowledge is presently available on the KSH enzyme. Here, we report for the first time the identification and ...

journal_title：Molecular microbiology

authors： van der Geize R,Hessels GI,van Gerwen R,van der Meijden P,Dijkhuizen L

更新日期：2002-08-01 00:00:00

abstract：:RelA and SpoT of Gram-negative organisms critically regulate cellular levels of (p)ppGpp. Here, we have dissected the spoT gene function of the cholera pathogen Vibrio cholerae by extensive genetic analysis. Unlike Escherichia coli, V. choleraeDeltarelADeltaspoT cells accumulated (p)ppGpp upon fatty acid or glucose st...

journal_title：Molecular microbiology

authors： Das B,Pal RR,Bag S,Bhadra RK

更新日期：2009-04-01 00:00:00

abstract：:Fungal cellulolytic and hemicellulolytic enzymes are promising tools for industrial hydrolysis of cellulosic biomass; however, the regulatory network underlying their production is not well understood. The recent discovery of the transcriptional activators ClrB and McmA in Aspergillus nidulans implied a novel regulato...

journal_title：Molecular microbiology

authors： Li N,Kunitake E,Aoyama M,Ogawa M,Kanamaru K,Kimura M,Koyama Y,Kobayashi T

更新日期：2016-12-01 00:00:00

abstract：:The syrD gene of Pseudomonas syringae pathovar syringae strain B301D-R was characterized and sequenced. The syrD open reading frame is 1695 bp long and encodes a predicted protein, SyrD, of approximately 63 kDa. Database searches revealed that SyrD shares a high degree of similarity with the ATP-binding cassette (ABC)...

journal_title：Molecular microbiology

authors： Quigley NB,Mo YY,Gross DC

更新日期：1993-08-01 00:00:00

abstract：:Vegetative replication and partitioning of many plasmids and some chromosomes of alphaproteobacteria are directed by their repABC operons. RepA and RepB proteins direct the partitioning of replicons to daughter cells, while RepC proteins are replication initiators, although they do not resemble any characterized repli...

journal_title：Molecular microbiology

authors： Pinto UM,Flores-Mireles AL,Costa ED,Winans SC

更新日期：2011-09-01 00:00:00

abstract：:Soupene et al. [J. Bacteriol. (2003) 185 5611-5626] made the unexpected observation that the presence of a mutation, in the gene for the N-acetylglucosamine repressor, nagC, increased the growth rate of Escherichia coli MG1655 on galactose, an unrelated sugar. We have found that NagC, binds to a single, high-affinity ...

journal_title：Molecular microbiology

authors： El Qaidi S,Allemand F,Oberto J,Plumbridge J

更新日期：2009-01-01 00:00:00

abstract：:citS and citT genes encoding a new two-component system were identified in the 71 degrees region between the pel and citM loci on the Bacillus subtilis chromosome. citS- and citT-deficient strains were unable to grow on minimal plates including citrate as a sole carbon source. In addition, a strain deficient in citM, ...

journal_title：Molecular microbiology

authors： Yamamoto H,Murata M,Sekiguchi J

更新日期：2000-08-01 00:00:00

abstract：:Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...

journal_title：Molecular microbiology

authors： Grainger DC,Goldberg MD,Lee DJ,Busby SJ

更新日期：2008-06-01 00:00:00

abstract：:We have examined the role of the F-plasmid TraV outer membrane lipoprotein in the assembly of F-pili. Yeast two-hybrid analysis with a traV bait repeatedly identified traK, which is predicted to encode a periplasmic protein, among positive prey plasmids. A traK bait in turn identified traV and traB, which is predicted...

journal_title：Molecular microbiology

authors： Harris RL,Hombs V,Silverman PM

更新日期：2001-11-01 00:00:00

abstract：:Many filamentous cyanobacteria are capable of gliding motility by an undefined mechanism. Within the heterocyst-forming clades, some strains, such as the Nostoc spp. and Fisherella spp., are motile only as specialized filaments termed hormogonia. Here we report on the phenotype of inactivation of a methyl-accepting ch...

journal_title：Molecular microbiology

authors： Risser DD,Meeks JC

更新日期：2013-02-01 00:00:00

abstract：:Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to any of these factors....

journal_title：Molecular microbiology

authors： Zawilak-Pawlik A,Kois A,Stingl K,Boneca IG,Skrobuk P,Piotr J,Lurz R,Zakrzewska-Czerwińska J,Labigne A

更新日期：2007-08-01 00:00:00

abstract：:Superoxide dismutase, catalase, glutathione peroxidase and peroxiredoxins form an antioxidant network protecting cells against reactive oxygen species (ROS). Catalase is a potent H2O2-detoxifying enzyme, which is unexpectedly absent in some members of the Kinetoplastida and Apicomplexa, but present in Toxoplasma gondi...

journal_title：Molecular microbiology

authors： Ding M,Kwok LY,Schlüter D,Clayton C,Soldati D

更新日期：2004-01-01 00:00:00

abstract：:Group II introns are mobile retroelements that invade their cognate intron-minus gene in a process known as retrohoming. They can also retrotranspose to ectopic sites at low frequency. Previous studies of the Lactococcus lactis intron Ll.LtrB indicated that in its native host, as in Escherichia coli, retrohoming occur...

journal_title：Molecular microbiology

authors： Coros CJ,Landthaler M,Piazza CL,Beauregard A,Esposito D,Perutka J,Lambowitz AM,Belfort M

更新日期：2005-04-01 00:00:00

abstract：:The stable maintenance of low-copy-number plasmids requires active partitioning, with the most common mechanism in prokaryotes involving the ATPase ParA. ParA proteins undergo intricate spatiotemporal relocations across the nucleoid, dynamics that function to position plasmids at equally spaced intervals. This spacing...

journal_title：Molecular microbiology

authors： Howard M,Gerdes K

更新日期：2010-10-01 00:00:00

abstract：:Crown gall tumorigenesis by Agrobacterium tumefaciens requires the co-ordinate transcriptional induction of a set of pathogenesis genes. At least three classes of environmental stimuli act synergistically to induce these genes: (i) monocyclic aromatic hydrocarbons such as acetosyringone, coniferyl alcohol, and vanilli...

journal_title：Molecular microbiology

authors： Winans SC

更新日期：1991-10-01 00:00:00

abstract：:Colonies of strains carrying a stable lambdaplacMu15 translational fusion displayed sharply defined intense staining at the centre on Xgal medium. The fusion was in fiu (ferric ion uptake), encoding an iron-regulated outer membrane protein (IROMP) controlled via four overlapping ferric uptake regulator (Fur) boxes in ...

journal_title：Molecular microbiology

authors： Newman DL,Shapiro JA

更新日期：1999-07-01 00:00:00

abstract：:Peptide transport in microorganisms is important for nutrition of the cell and various signalling processes including regulation of gene expression, sporulation, chemotaxis, competence and virulence development. Peptide transport is mediated via different combinations of ion-linked and ATP-binding cassette (ABC) trans...

journal_title：Molecular microbiology

authors： Doeven MK,Kok J,Poolman B

更新日期：2005-08-01 00:00:00

abstract：:The wrinkly spreader (WS) genotype of Pseudomonas fluorescens SBW25 colonizes the air-liquid interface of spatially structured microcosms resulting in formation of a thick biofilm. Its ability to colonize this niche is largely due to overproduction of a cellulosic polymer, the product of the wss operon. Chemical analy...

journal_title：Molecular microbiology

authors： Spiers AJ,Bohannon J,Gehrig SM,Rainey PB

更新日期：2003-10-01 00:00:00

abstract：:Sinorhizobium meliloti and host legumes enter into a nitrogen-fixing, symbiotic relationship triggered by an exchange of signals between bacteria and plant. S. meliloti produces Nod factor, which elicits the formation of nodules on plant roots, and succinoglycan, an exopolysaccharide that allows for bacterial invasion...

journal_title：Molecular microbiology

authors： Wells DH,Long SR

更新日期：2002-03-01 00:00:00

abstract：:Attaching and effacing (A/E) lesions and actin polymerization, the hallmark of enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC) and Citrobacter rodentium (CR) infections, are dependent on the effector Tir. Phosphorylation of Tir(EPEC/CR) Y474/1 leads to recruitment of Nck and neural Wiskott-A...

journal_title：Molecular microbiology

authors： Crepin VF,Girard F,Schüller S,Phillips AD,Mousnier A,Frankel G

更新日期：2010-01-01 00:00:00

abstract：:In the intracellular pathogen Brucella abortus, the general stress response (GSR) signalling system determines survival under acute stress conditions in vitro, and is required for long-term residence in a mammalian host. To date, the identity of the Brucella sensor kinase(s) that function to perceive stress and direct...

journal_title：Molecular microbiology

authors： Kim HS,Willett JW,Jain-Gupta N,Fiebig A,Crosson S

更新日期：2014-11-01 00:00:00

abstract：:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (sigma) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cau...

journal_title：Molecular microbiology

authors： Manganelli R,Voskuil MI,Schoolnik GK,Dubnau E,Gomez M,Smith I

更新日期：2002-07-01 00:00:00

abstract：:The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

journal_title：Molecular microbiology

authors： Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

更新日期：2009-06-01 00:00:00

abstract：:The AMA1 sequence is an efficient plasmid replicator and transformation enhancer in Aspergillus nidulans. It comprises two long perfect inverted repeats (MATE elements) flanking a short, unique, central spacer. Subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is ne...

journal_title：Molecular microbiology

authors： Aleksenko A,Gems D,Clutterbuck J

更新日期：1996-04-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:The heterotrimeric G-protein pathway is a ubiquitous eukaryotic signalling module that is known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1, a gene encoding a G-protein alpha subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs to...

journal_title：Molecular microbiology

authors： Latijnhouwers M,Ligterink W,Vleeshouwers VG,van West P,Govers F

更新日期：2004-02-01 00:00:00

abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...

journal_title：Molecular microbiology

authors： Veith A,Klingl A,Zolghadr B,Lauber K,Mentele R,Lottspeich F,Rachel R,Albers SV,Kletzin A

更新日期：2009-07-01 00:00:00

abstract：:Pathogenic enterotoxigenic Escherichia coli (ETEC) are the major bacterial cause of diarrhea in young children in developing countries and in travelers, causing significant mortality in children. Adhesive fimbriae are a prime virulence factor for ETEC, initiating colonization of the small intestinal epithelium. Simila...

journal_title：Molecular microbiology

authors： Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

更新日期：2015-01-01 00:00:00

abstract：:The transport of iron complexes through outer membrane transporters from Gram-negative bacteria is highly dependent on the TonB system. Together, the three components of the system, TonB, ExbB and ExbD, energize the transport of iron complexes through the outer membrane by utilizing the proton motive force across the ...

journal_title：Molecular microbiology

authors： Garcia-Herrero A,Peacock RS,Howard SP,Vogel HJ

更新日期：2007-11-01 00:00:00

abstract：:The CovR/S two-component system regulates the transcription of many genes that are crucial for the virulence of Streptococcus pyogenes (group A Streptococcus, GAS). Previously, we demonstrated that one gene repressed directly by CovR is rivR, which encodes a member of the RofA-like family of transcriptional regulators...

journal_title：Molecular microbiology

authors： Roberts SA,Scott JR

更新日期：2007-12-01 00:00:00