Abstract：
:In the intracellular pathogen Brucella abortus, the general stress response (GSR) signalling system determines survival under acute stress conditions in vitro, and is required for long-term residence in a mammalian host. To date, the identity of the Brucella sensor kinase(s) that function to perceive stress and directly activate GSR signalling have remained undefined. We demonstrate that the flavin-binding sensor histidine kinase, LovhK (bab2_0652), functions as a primary B. abortus GSR sensor. LovhK rapidly and specifically phosphorylates the central GSR regulator, PhyR, and activates transcription of a set of genes that closely overlaps the known B. abortus GSR regulon. Deletion of lovhK severely compromises cell survival under defined oxidative and acid stress conditions. We further show that lovhK is required for cell survival during the early phase of mammalian cell infection and for establishment of long-term residence in a mouse infection model. Finally, we present evidence that particular regions of primary structure within the two N-terminal PAS domains of LovhK have distinct sensory roles under specific environmental conditions. This study elucidates new molecular components of a conserved signalling pathway that regulates B. abortus stress physiology and infection biology.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Kim HS,Willett JW,Jain-Gupta N,Fiebig A,Crosson Sdoi
10.1111/mmi.12809subject
Has Abstractpub_date
2014-11-01 00:00:00pages
913-25issue
4eissn
0950-382Xissn
1365-2958journal_volume
94pub_type
杂志文章abstract：:SpsA, a pneumococcal surface protein belonging to the family of choline-binding proteins, interacts specifically with secretory immunglobulin A (SIgA) via the secretory component (SC). SIgA and free SC from mouse, rat, rabbit and guinea-pig failed to interact with SpsA indicating species-specific binding to human SIgA...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01897.x
更新日期：2000-05-01 00:00:00
abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04806.x
更新日期：2005-10-01 00:00:00
abstract：:The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14236
更新日期：2019-06-01 00:00:00
abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06689.x
更新日期：2009-06-01 00:00:00
abstract：:Of the four Plasmodium species that routinely cause malaria in humans, Plasmodium falciparum is responsible for the majority of malaria mortality and consequently gets most of the headlines. Outside Africa, however, more malaria cases are caused by its distant cousin Plasmodium vivax, resulting in a daunting morbidity...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04518.x
更新日期：2005-03-01 00:00:00
abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04816.x
更新日期：2005-10-01 00:00:00
abstract：:Penicillium marneffei is a thermally dimorphic opportunistic human pathogen with a saprophytic filamentous hyphal form at 25 degrees C and a pathogenic unicellular yeast form at 37 degrees C. During infection. P. marneffei yeast cells exist intracellularly in macrophages. To cope with nutrient deprivation during the i...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05477.x
更新日期：2006-12-01 00:00:00
abstract：:Saccharomyces cerevisiae neutral trehalase, encoded by NTH1, controls trehalose hydrolysis in response to multiple stress conditions, including nutrient limitation. The presence of three stress responsive elements (STREs, CCCCT) in the NTH1 promoter suggested that the transcriptional activator proteins Msn2 and Msn4, ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01706.x
更新日期：2000-01-01 00:00:00
abstract：:SigD and SigE (Salmonella invasion gene) are proteins needed for optimal invasion of Salmonella typhimurium into eukaryotic cells in vitro. SigD is a secreted protein and SigE is a putative chaperone required for SigD stability and/or secretion. SigD is secreted by a type III secretion apparatus encoded within a patho...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01772.x
更新日期：2000-02-01 00:00:00
abstract：:The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably se...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2002.02922.x
更新日期：2002-05-01 00:00:00
abstract：:The Bordetella pertussis wlb locus (wlbpe, formerly bpl) is required for the biosynthesis of a trisaccharide that, when attached to the B. pertussis lipopolysaccharide (LPS) core (band B), generates band A LPS. The equivalent loci in Bordetella bronchiseptica (wlbbr) and Bordetella parapertussis (wlbpa) were identifie...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1998.00878.x
更新日期：1998-07-01 00:00:00
abstract：:We describe a chemotactic-like response of Escherichia coli strains lacking most of the known chemotaxis machinery but containing high levels of the response regulator CheY. The bacteria accumulated in aspartate-containing capillaries, they formed rings on tryptone-containing semisolid agar, and the probability of cou...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01251.x
更新日期：1999-02-01 00:00:00
abstract：:Cholesterol catabolism is widespread in actinobacteria and is critical for Mycobacterium tuberculosis (Mtb) virulence. Catabolism of steroid nucleus rings C and D is poorly understood: it is initiated by the CoA thioesterification of 3aα-H-4α(3'-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) by FadD3, whose gen...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12340
更新日期：2013-09-01 00:00:00
abstract：:Mutants (glk) of Streptomyces coelicolor A3(2) that are resistant to the non-utilizable glucose analogue 2-deoxyglucose are deficient in glucose kinase activity, defective in glucose repression, and usually unable to utilize glucose. A 2.9 kb BclI fragment, previously shown to restore a wild-type phenotype to a glk de...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01463.x
更新日期：1992-10-01 00:00:00
abstract：:The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al., 1988; Kimman et al., 1987). In this report the toxin-encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal-b...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb00652.x
更新日期：1990-05-01 00:00:00
abstract：:The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% ide...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01511.x
更新日期：1992-03-01 00:00:00
abstract：:Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03907.x
更新日期：2004-02-01 00:00:00
abstract：:Initiation of translation involves recognition of the start codon by the initiator tRNA in the 30S subunit. To investigate the role of ribosomal RNA (rRNA) in this process, we isolated a number of 16S rRNA mutations that increase translation from the non-canonical start codon AUC. These mutations cluster to distinct r...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06598.x
更新日期：2009-03-01 00:00:00
abstract：:Protein quality control and proteolysis are involved in cell maintenance and environmental acclimatization in bacteria and eukaryotes. The AAA protease FtsH2 of the cyanobacterium Synechocystis sp. PCC 6803 was identified during a screening for mutants impaired in osmoregulation. The ftsH2(-) mutant was salt sensitive...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05495.x
更新日期：2007-01-01 00:00:00
abstract：:It has been previously reported that the three-dimensional structures of the NAD-binding and catalytic site of bacterial toxins with ADP-ribosylating activity are superimposable, and that the key amino acids for the enzymatic activity are conserved. The model includes an NAD-binding and catalytic site formed by an alp...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2958.1996.321396.x
更新日期：1996-08-01 00:00:00
abstract：:The aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18. The nucleotide sequence of the P. multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01794.x
更新日期：1992-12-01 00:00:00
abstract：:Spatio-temporal oscillations of the Min proteins are essential for selecting the cell division site in Escherichia coli. These oscillations are a key example of a biological phenomenon that can only be understood on a systems level rather than on the level of its individual components. Here, we review the key concepts...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2007.05607.x
更新日期：2007-03-01 00:00:00
abstract：:Listeria monocytogenes grows in the cytosol of mammalian cells and spreads from cell to cell without exiting the intracellular milieu. During cell-cell spread, bacteria become transiently entrapped in double-membrane vacuoles. Escape from these vacuoles is mediated in part by a bacterial phospholipase C (PC-PLC), whos...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01708.x
更新日期：2000-01-01 00:00:00
abstract：:The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2002.02818.x
更新日期：2002-03-01 00:00:00
abstract：:Initiation of Escherichia coli chromosome replication is controlled by the DnaA initiator protein. Both rifampicin-mediated inhibition of transcription and ppGpp-induced changes in global transcription stops replication at the level of initiation. Here, we show that continued DnaA protein synthesis allows for replicat...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14531
更新日期：2020-12-01 00:00:00
abstract：:The cell envelope of mycobacteria is a complex multilaminar structure that protects the cell from stresses encountered in the environment, and plays an important role against the bactericidal activity of immune system cells. The outermost layer of the mycobacterial envelope typically contains species-specific glycolip...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04847.x
更新日期：2005-10-01 00:00:00
abstract：:It is now well established that the sigma S subunit of RNA polymerase is a master regulator in a complex regulatory network that governs the expression of many stationary-phase-inducible genes in Escherichia coli. In this review, more recent findings will be summarized that demonstrate that sigma S also acts as a glob...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2958.1996.511405.x
更新日期：1996-09-01 00:00:00
abstract：:Strains of Neisseria meningitidis express one of two porin proteins. These porins have been identified as the class 2 and class 3 proteins, and express serotype-specific epitopes. The gene for the class 3 protein was amplified by the polymerase chain reaction from the DNA of a serotype 4 strain as a 1025 bp fragment. ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01784.x
更新日期：1992-12-01 00:00:00
abstract：:We have determined the sizes of the chromosomes of six Bacillus cereus strains (range 2.4-4.3 Mb) and constructed a physical map of the smallest B. cereus chromosome (2.4 Mb). This map was compared to those of the chromosomes of four B. cereus strains and one B. thuringiensis strain previously determined to be 5.4-6.3...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb00411.x
更新日期：1994-07-01 00:00:00
abstract：:Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one of the high-Mr PBPs which appears to be altered both in resistant clinical isolates, and in cefotaxime-resistant la...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00115.x
更新日期：1989-10-01 00:00:00
