Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus.


:The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably serve unrelated functions in the cells. The chromosomally encoded genetic determinants for production of the pheromones have been identified and designated camE (encoding cAM373) and camS (encoding staph-cAM373). Truncated and full-length clones of camE were generated in Escherichia coli, in which cAM373 activity was expressed. In E. faecalis, insertional inactivation in the middle of camE had no detectable phenotypic effects on the pheromone system. Establishment of an in frame translation stop codon within the signal sequence resulted in reduction of cAM373 activity to 3% of normal levels. The camS determinant has homologues in Staphylococcus epidermidis, Bacillus subtilis and Listeria monocytogenes; however, corresponding heptapeptides present within those sequences do not resemble staph-cAM373 closely. The particular significance of staph-cAM373 as a potential intergeneric inducer of transfer-proficient genetic elements is discussed.


Mol Microbiol


Molecular microbiology


Flannagan SE,Clewell DB




Has Abstract


2002-05-01 00:00:00














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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gostick DO,Griffin HG,Shearman CA,Scott C,Green J,Gasson MJ,Guest JR

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    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lange U,Hausner W

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    pub_type: 杂志文章


    authors: Bennett HJ,Pearce DM,Glenn S,Taylor CM,Kuhn M,Sonenshein AL,Andrew PW,Roberts IS

    更新日期:2007-03-01 00:00:00