Abstract:
:Saccharomyces cerevisiae responds to environ-mental stimuli such as an exposure to pheromone or to hexoses after carbon source limitation with a transient elevation of cytosolic calcium (TECC) response. In this study, we examined whether hexose transport and phosphorylation are necessary for the TECC response. We found that a mutant strain lacking most of the known hexose transporters was unable to carry out the TECC response when exposed to glucose. A mutant strain that lacked the ability to phosphorylate glucose was unable to respond to glucose addition, but displayed a normal TECC response after the addition of galactose. These results indicate that hexose uptake and phosphorylation are required to trigger the hexose-induced TECC response. We also found that the TECC response was significantly smaller than normal when the level of environmental calcium was reduced, and was abolished in a mid1 mutant that lacked a subunit of the high-affinity calcium channel of the yeast plasma membrane. These results indicate that most or all of the TECC response is mediated by an influx of calcium from the extracellular space. Our results indicate that this transient increase in plasma membrane calcium permeability may be linked to the accumulation of Glc-1-P (or a related glucose metabolite) in yeast.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Tökés-Füzesi M,Bedwell DM,Repa I,Sipos K,Sümegi B,Rab A,Miseta Adoi
10.1046/j.1365-2958.2002.02956.xsubject
Has Abstractpub_date
2002-06-01 00:00:00pages
1299-308issue
5eissn
0950-382Xissn
1365-2958pii
2956journal_volume
44pub_type
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