Expanded linkage map of Erwinia chrysanthemi strain 3937.

abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

journal_title：Molecular microbiology

authors： Quinn FD,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Amino acid sequence analyses have indicated that the amino-terminal part of bacteriophage PRD1 structural protein P7 carries a conserved transglycosylase domain. We analysed wild-type PRD1 and different mutant particles in zymograms and found a glycolytic activity that was associated with protein P7. This is the first...

journal_title：Molecular microbiology

authors： Rydman PS,Bamford DH

更新日期：2000-07-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:A 34 kb fragment of the Nocardia lactamdurans DNA carrying the cluster of early cephamycin biosynthetic genes was cloned in lambda EMBL3 by hybridization with probes internal to the pcbAB and pcbC genes of Penicillium chrysogenum and Streptomyces griseus. The pcbAB and pcbC genes were found to be closely linked togeth...

journal_title：Molecular microbiology

authors： Coque JJ,Martín JF,Calzada JG,Liras P

更新日期：1991-05-01 00:00:00

abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

journal_title：Molecular microbiology

authors： Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

更新日期：2009-06-01 00:00:00

abstract：:Slipped-strand mispairing at sites containing so-called coding repeats (CRs) can lead to phase variation of surface proteins in Gram-negative bacteria. This mechanism, believed to contribute to virulence, has so far not been identified in a Gram-positive bacterium. In the genome of the Gram-positive human pathogen Str...

journal_title：Molecular microbiology

authors： Rasmussen M,Björck L

更新日期：2001-06-01 00:00:00

abstract：:Crs5 is a Saccharomyces cerevisiae Metallothionein (MT), non-homologous to the paradigmatic Cu-thionein Cup1. Although considered a secondary copper-resistance agent, we show here that it determines survival under zinc overload in a CUP1-null background. Its overexpression prevents the deleterious effects exhibited by...

journal_title：Molecular microbiology

authors： Pagani A,Villarreal L,Capdevila M,Atrian S

更新日期：2007-01-01 00:00:00

abstract：:HapR is a quorum sensing-regulated transcription factor that represses the virulence cascade in Vibrio cholerae by binding to a specific site centred at -71 in the aphA promoter, ultimately preventing activation of the tcpPH promoter on the Vibrio pathogenicity island. In an effort to elucidate the mechanism by which ...

journal_title：Molecular microbiology

authors： Lin W,Kovacikova G,Skorupski K

更新日期：2007-05-01 00:00:00

abstract：:The secretion of numerous proteins during vegetative growth of Myxococcus xanthus, and the multicellular development cycle induced upon starvation of these bacteria, are partially interrelated in so far as mutants impaired in extracellular protein production are unable to undergo development. We have cloned and sequen...

journal_title：Molecular microbiology

authors： Laval-Favre K,Letouvet-Pawlak B,Friedrich T,Alexandre J,Guespin-Michel JF

更新日期：1997-03-01 00:00:00

abstract：:Oligomerization of the initiator protein, DnaA, on the origin of replication (oriC) is crucial for initiation of DNA replication. Studies in Escherichia coli (Gram-negative) have revealed that binding of DnaA to ATP, but not hydrolysis of ATP, is sufficient to promote DnaA binding, oligomerization and DNA strand separ...

journal_title：Molecular microbiology

authors： Madiraju MV,Moomey M,Neuenschwander PF,Muniruzzaman S,Yamamoto K,Grimwade JE,Rajagopalan M

更新日期：2006-03-01 00:00:00

abstract：:We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine...

journal_title：Molecular microbiology

authors： Green R,Hanfrey CC,Elliott KA,McCloskey DE,Wang X,Kanugula S,Pegg AE,Michael AJ

更新日期：2011-08-01 00:00:00

abstract：:A mechanistic hypothesis for the origin of the three domains of life is proposed. A population of evolving pre-cells is suggested to have had a membrane of a racemate of chiral lipids that continuously underwent spontaneous symmetry breaking by spatial phase segregation into two enantiomerically enriched membrane doma...

journal_title：Molecular microbiology

authors： Wächtershäuser G

更新日期：2003-01-01 00:00:00

abstract：:Biofilm formation in Bacillus subtilis requires expression of the eps and tapA-sipW-tasA operons to synthesize the extracellular matrix components, extracellular polysaccharide and TasA amyloid proteins, respectively. Expression of both operons is inhibited by the DNA-binding protein master regulator of biofilm format...

journal_title：Molecular microbiology

authors： Winkelman JT,Bree AC,Bate AR,Eichenberger P,Gourse RL,Kearns DB

更新日期：2013-06-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

journal_title：Molecular microbiology

authors： Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

更新日期：2009-06-01 00:00:00

abstract：:Invasive sporozoite and merozoite stages of malaria parasites that infect mammals enter and subsequently reside in hepatocytes and red blood cells respectively. Each invasive stage may exhibit unique adaptations that allow it to interact with and survive in its distinct host cell environment, and these adaptations are...

journal_title：Molecular microbiology

authors： Kaiser K,Matuschewski K,Camargo N,Ross J,Kappe SH

更新日期：2004-03-01 00:00:00

abstract：:Progress towards understanding the molecular basis of virulence in Clostridium difficile has been hindered by the lack of effective gene transfer systems. We have now, for the first time, developed procedures that may be used to introduce autonomously replicating vectors into this organism through their conjugative, o...

journal_title：Molecular microbiology

authors： Purdy D,O'Keeffe TA,Elmore M,Herbert M,McLeod A,Bokori-Brown M,Ostrowski A,Minton NP

更新日期：2002-10-01 00:00:00

abstract：:The structural complexity of a ligand in association with the molecular chaperones SecB and SecA was investigated using three species of precursor maltose-binding protein, which differ in their stability as a result of an amino acid substitution in each that affects the rate of folding of the polypeptide. In the prese...

journal_title：Molecular microbiology

authors： de Cock H,Randall LL

更新日期：1998-01-01 00:00:00

abstract：:The gltX gene, encoding the glutamyl-tRNA synthetase (GluRS), and the valU operon, whose transcripts contain three tRNAVal/UAC and one tRNALys/UUU, are adjacent and divergently transcribed. It is the only known case of adjacent genes encoding an aminoacyl-tRNA synthetase and a tRNA precursor in Escherichia coli. The g...

journal_title：Molecular microbiology

authors： Champagne N,Lapointe J

更新日期：1998-03-01 00:00:00

abstract：:Sequence determination of a region downstream from the vanXYc gene in Enterococcus gallinarum BM4174 revealed an open reading frame, designated vanT, that encodes a 698-amino-acid polypeptide with an amino-terminal domain containing 10 predicted transmembrane segments. The protein contained a highly conserved pyridoxa...

journal_title：Molecular microbiology

authors： Arias CA,Martín-Martinez M,Blundell TL,Arthur M,Courvalin P,Reynolds PE

更新日期：1999-03-01 00:00:00

abstract：:Pathogenic strains of Yersinia deploy a type III secretion system to inject the potent tyrosine phosphatase YopH into host cells, where it dephosphorylates focal adhesion-associated substrates. The amino-terminal, non-catalytic domain of YopH is bifunctional; it is essential for the secretion and binding of the specif...

journal_title：Molecular microbiology

authors： Smith CL,Khandelwal P,Keliikuli K,Zuiderweg ER,Saper MA

更新日期：2001-11-01 00:00:00

abstract：:DNA double-strand break repair can be accomplished by homologous recombination when a sister chromatid or a homologous chromosome is available. However, the study of sister chromatid double-strand break repair in prokaryotes is complicated by the difficulty in targeting a break to only one copy of two essentially iden...

journal_title：Molecular microbiology

authors： Cromie GA,Leach DR

更新日期：2001-08-01 00:00:00

abstract：:Comparison of the Leishmania infantum genome with Leishmania braziliensis and Leishmania major genomes has identified 25 L. infantum species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To determine whether these L. infantum species-specific genes are involved in visceral Leishmania i...

journal_title：Molecular microbiology

authors： Zhang WW,Matlashewski G

更新日期：2010-07-01 00:00:00

abstract：:Various features of the regulation of pathways for biodegradation of recalcitrant compounds by Pseudomonas provide insights into the mechanisms by which operons evolve to acquire conditionally active promoters that permit the corresponding genes to be transcribed only when required. The "regulatory noise hypothesis' p...

journal_title：Molecular microbiology

authors： de Lorenzo V,Pérez-Martín J

更新日期：1996-03-01 00:00:00

abstract：:Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or...

journal_title：Molecular microbiology

authors： Rozalski A,Brade L,Kosma P,Moxon R,Kusumoto S,Brade H

更新日期：1997-02-01 00:00:00

abstract：:The nucleotide sequence of the mip genes and their inferred amino acid sequences were determined from 35 Legionella species and compared with the published sequences for L. pneumophila, L. micdadei and L. longbeachae. The sequences were 69-97% conserved at the nucleotide level and 82-99% at the amino acid level, with ...

journal_title：Molecular microbiology

authors： Ratcliff RM,Donnellan SC,Lanser JA,Manning PA,Heuzenroeder MW

更新日期：1997-09-01 00:00:00

abstract：:The assembly of proteins that display complementary activities into supramolecular intra- and extracellular complexes is central to cellular function. One such nanomachine of considerable biological and industrial significance is the plant cell wall degrading apparatus of anaerobic bacteria termed the cellulosome. The...

journal_title：Molecular microbiology

authors： Gilbert HJ

更新日期：2007-03-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteoba...

journal_title：Molecular microbiology

authors： Pini F,Frage B,Ferri L,De Nisco NJ,Mohapatra SS,Taddei L,Fioravanti A,Dewitte F,Galardini M,Brilli M,Villeret V,Bazzicalupo M,Mengoni A,Walker GC,Becker A,Biondi EG

更新日期：2013-10-01 00:00:00

abstract：:Interpretation of the constantly expanding body of genomic information requires that the function of each gene be established. Here we report the genomic analysis and structural modelling of a previously uncharacterized redox-metabolism protein UrdA (SO_4620) of Shewanella oneidensis MR-1, which led to a discovery of ...

journal_title：Molecular microbiology

authors： Bogachev AV,Bertsova YV,Bloch DA,Verkhovsky MI

更新日期：2012-12-01 00:00:00