Abstract:
:Enteropathogenic Escherichia coli (EPEC) elicit changes in host cell morphology and cause actin rearrangement, a phenotype that has commonly been referred to as attaching/effacing (AE) lesions. The ability of EPEC to induce AE lesions is dependent upon a type III protein secretion/translocation system that is encoded by genes clustered in a 35.6 kb DNA segment, named the locus of enterocyte effacement (LEE). We used transcriptional fusions between the green fluorescent protein (gfp) reporter gene and LEE genes rorf2, orf3, orf5, escJ, escV and eae, together with immunoblot analysis with antibodies against Tir, intimin, EspB and EspF, to analyse the genetic regulation of the LEE. The expression of all these LEE genes was strictly dependent upon the presence of a functional integration host factor (IHF). IHF binds specifically upstream from the ler (orf1) promoter and appears to activate expression of ler, orf3, orf5 and rorf2 directly. The ler-encoded Ler protein was involved in activating the expression of escJ, escV, tir, eae, espB and espF. Expression of both IHF and Ler was needed to elicit actin rearrangement associated with AE lesions. In conclusion, IHF directly activates the expression of the ler and rorf2 transcriptional units, and Ler in turn mediates the expression of the other LEE genes.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Friedberg D,Umanski T,Fang Y,Rosenshine Idoi
10.1046/j.1365-2958.1999.01655.xsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
941-52issue
5eissn
0950-382Xissn
1365-2958pii
mmi1655journal_volume
34pub_type
杂志文章abstract::We previously observed that hypoxia is an important component of host microenvironments during pulmonary fungal infections. However, mechanisms of fungal growth in these in vivo hypoxic conditions are poorly understood. Here, we report that mitochondrial respiration is active in hypoxia (1% oxygen) and critical for fu...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Molecular microbiology
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更新日期:2002-12-01 00:00:00
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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更新日期:2005-05-01 00:00:00
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更新日期:2009-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13345
更新日期:2016-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2007-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1989.tb00275.x
更新日期:1989-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1989-08-01 00:00:00
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更新日期:1998-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2958.1996.321396.x
更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01515.x
更新日期:1999-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14236
更新日期:2019-06-01 00:00:00
abstract::HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demon...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1994.tb00356.x
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00