Sequence, exon-intron organization, transcription and mutational analysis of prnA, the gene encoding the transcriptional activator of the prn gene cluster in Aspergillus nidulans.

abstract：:Apicomplexan parasites, including Toxoplasma gondii, apically attach to their host cells before invasion. Recent studies have implicated the contents of micronemes, which are small secretory organelles confined to the apical region of the parasite, in the process of host cell attachment. Here, we demonstrate that micr...

journal_title：Molecular microbiology

authors： Carruthers VB,Sibley LD

更新日期：1999-01-01 00:00:00

abstract：:Bacillus subtilis SpoIIE is a Ser protein phosphatase whose action on the phosphoprotein SpoIIAA triggers the cell type-specific activation of a sporulation transcription factor. Here we report that SpoIIE displays sequence similarity to the PP2C family of eukaryotic Ser/Thr protein phosphatases, and that residues com...

journal_title：Molecular microbiology

authors： Adler E,Donella-Deana A,Arigoni F,Pinna LA,Stragler P

更新日期：1997-01-01 00:00:00

abstract：:Bacterial cytokinesis is orchestrated by an assembly of essential cell division proteins that form a supramolecular structure known as the divisome. DivIB and its orthologue FtsQ are essential members of the divisome in Gram-positive and Gram-negative bacteria respectively. DivIB is a bitopic membrane protein composed...

journal_title：Molecular microbiology

authors： Wadsworth KD,Rowland SL,Harry EJ,King GF

更新日期：2008-03-01 00:00:00

abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...

journal_title：Molecular microbiology

authors： Delany I,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-12-01 00:00:00

abstract：:Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

journal_title：Molecular microbiology

authors： Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

更新日期：2009-11-01 00:00:00

abstract：:Various physiological stimuli trigger the conversion of noninfective Leishmania donovani promastigotes to the infective form. Here, we present the first evidence of the effect of glucose starvation, on virulence and survival of these parasites. Glucose starvation resulted in a decrease in metabolically active parasite...

journal_title：Molecular microbiology

authors： Saini S,Kumar Ghosh A,Singh R,Das S,Abhishek K,Kumar A,Verma S,Mandal A,Hasan Sardar A,Purkait B,Kumar A,Kumar Sinha K,Das P

更新日期：2016-12-01 00:00:00

abstract：:fliL is the first gene in a flagellar operon that specifies members of the switch complex and type III export system in Salmonella enterica and Escherichia coli, but no function has been ascribed to this gene thus far. Here we report that a fliL mutant is slightly impaired for swimming but completely defective in swar...

journal_title：Molecular microbiology

authors： Attmannspacher U,Scharf BE,Harshey RM

更新日期：2008-04-01 00:00:00

abstract：:Bacteria in terrestrial habitats frequently reside as biofilm communities on surfaces that are unsaturated, i.e. biofilms are covered in water films varying in thickness depending on the environmental conditions. Water availability in these habitats is influenced by the osmolarity of the water (solute stress) and by c...

journal_title：Molecular microbiology

authors： van de Mortel M,Halverson LJ

更新日期：2004-05-01 00:00:00

abstract：:Proteic toxin-antitoxin (TA) loci were first identified in bacterial plasmids, and they were regarded as involved in stable plasmid maintenance by a so-called 'addiction' mechanism. Later, chromosomally encoded TA loci were identified and their function ascribed to survival mechanisms when bacteria were subjected to s...

journal_title：Molecular microbiology

authors： Nieto C,Pellicer T,Balsa D,Christensen SK,Gerdes K,Espinosa M

更新日期：2006-02-01 00:00:00

abstract：:Streptococcus pneumoniae is one of the most important model organisms for studies on natural genetic transformation in bacteria. The prevalence of this gene exchange mechanism in the genus Streptococcus has not been subjected to systematic investigations, but it has been known for decades that only a few streptococcal...

journal_title：Molecular microbiology

authors： Håvarstein LS

更新日期：2010-11-01 00:00:00

abstract：:In Escherichia coli, bulky DNA lesions are repaired primarily by nucleotide excision repair (NER). Unrepaired lesions encountered by DNA polymerase at the replication fork create a blockage which may be relieved through RecF-dependent recombination. We have designed an assay to monitor the different mechanisms through...

journal_title：Molecular microbiology

authors： Bichara M,Pinet I,Lambert IB,Fuchs RP

更新日期：2007-07-01 00:00:00

abstract：:Many proteins have developed the potential to sequester a client polypeptide chain in its various folding states as a specific intermolecular ligand and, thus, exhibit the properties of a holding chaperone. The resulting complexes can be of a diverse nature in terms of structure and reaction dynamics and are character...

journal_title：Molecular microbiology

authors： Fischer G,Wawra S

更新日期：2006-09-01 00:00:00

abstract：:Candida albicans hypha formation which has been stimulated via the Ras1-cAMP-Efg1 signalling cascade is inhibited by farnesol, a C. albicans autoregulatory factor, and small molecules such as dodecanol. In cultures containing farnesol or dodecanol, hypha formation was restored upon addition of dibutyryl-cAMP. The CAI4...

journal_title：Molecular microbiology

authors： Davis-Hanna A,Piispanen AE,Stateva LI,Hogan DA

更新日期：2008-01-01 00:00:00

abstract：:The genome of the Bacillus subtilis 168-type strain contains 10 ribosomal RNA (rRNA) operons. In the intergenic spacer region (ISR) between the 16S and 23S rRNA genes, five rRNA operons, rrnI-H-G and rrnJ-W, lack a trinucleotide signature region. Precise determination of molecular weight (MW), using electrospray mass ...

journal_title：Molecular microbiology

authors： Shaver YJ,Nagpal ML,Fox KF,Rudner R,Fox A

更新日期：2001-10-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces type 4 fimbriae which promote adhesion to epithelial cells and are associated with a form of surface translocation called twitching motility. Transposon mutagenesis was used to identify loci required for fimbrial assembly or function by screening for mutants t...

journal_title：Molecular microbiology

authors： Martin PR,Watson AA,McCaul TF,Mattick JS

更新日期：1995-05-01 00:00:00

abstract：:srfA is a locus required for the production of the lipopeptide antibiotic surfactin. This locus is also necessary for efficient sporulation and competence development. Mutations in the 5' portion of the srfA operon affect all three of these processes, whereas mutations in the 3' portion of srfA only affect sporulation...

journal_title：Molecular microbiology

authors： van Sinderen D,Galli G,Cosmina P,de Ferra F,Withoff S,Venema G,Grandi G

更新日期：1993-05-01 00:00:00

abstract：:Invasive sporozoite and merozoite stages of malaria parasites that infect mammals enter and subsequently reside in hepatocytes and red blood cells respectively. Each invasive stage may exhibit unique adaptations that allow it to interact with and survive in its distinct host cell environment, and these adaptations are...

journal_title：Molecular microbiology

authors： Kaiser K,Matuschewski K,Camargo N,Ross J,Kappe SH

更新日期：2004-03-01 00:00:00

abstract：:Ribonucleotide reductases supply cells with their deoxyribonucleotides. Three enzyme types are known, classes I, II and III. Class II enzymes are anaerobic whereas class I enzymes are aerobic, and so class I and II enzymes are often produced by the same organism under opposing oxygen regimes. Escherichia coli contains...

journal_title：Molecular microbiology

authors： Andrews SC

更新日期：2011-04-01 00:00:00

abstract：:Understanding the mechanisms of polysialic acid synthesis in Escherichia coli K1 requires a molecular description of the polymerase complex. Since the number of potential models explaining polysialic acid assembly would be constrained if only one sialyltransferase were required for this process, the phenotypes of a si...

journal_title：Molecular microbiology

authors： Steenbergen SM,Vimr ER

更新日期：1990-04-01 00:00:00

abstract：:The alternative sigma factor sigma 54 is required for transcription of nitrogen fixation genes in Klebsiella pneumoniae and other diazotrophs. The nif genes, and other E sigma 54-dependent genes whose products are necessary for a wide range of processes, are postively regulated. A unifying model that is well supported...

journal_title：Molecular microbiology

authors： Buck M,Cannon W

更新日期：1992-06-01 00:00:00

abstract：:We isolated and characterized three spontaneous mutations leading to trimethylamine N-oxide (TMAO)-independent expression of the tor operon encoding the TMAO-reductase anaerobic respiratory system in Escherichia coli. The mutations lie in a new for regulatory gene, the torS gene, which probably encodes a sensor protei...

journal_title：Molecular microbiology

authors： Jourlin C,Bengrine A,Chippaux M,Méjean V

更新日期：1996-06-01 00:00:00

abstract：:The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

journal_title：Molecular microbiology

authors： Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

更新日期：2011-08-01 00:00:00

abstract：:Thymidylate, an essential building block of DNA, is synthesized either from deoxyuridylate by thymidylate synthase (TS) or thymidine (dT) by thymidine kinase (TK). Thymidylate kinase (TMPK) phosphorylates dTMP to dTTP. Thymidine phosphorylase (TP) catalyses reversible phosphorolysis of dT. Using transposon mutagenesis...

journal_title：Molecular microbiology

authors： Wang L,Hames C,Schmidl SR,Stülke J

更新日期：2010-09-01 00:00:00

abstract：:Photorhabdus are bacteria found colonizing the gut of a specialized stage of the nematode Heterorhabditis, called the infective juvenile (IJ). The IJ is a free-living stage of the nematode that seeks out and infects insect larvae. Once inside the insect the IJ release Photorhabdus into the haemolymph where the bacteri...

journal_title：Molecular microbiology

authors： Watson RJ,Joyce SA,Spencer GV,Clarke DJ

更新日期：2005-05-01 00:00:00

abstract：:N-linked glycosylation is recognized as an important post-translational modification across all three domains of life. However, the understanding of the genetic pathways for the assembly and attachment of N-linked glycans in eukaryotic and bacterial systems far outweighs the knowledge of comparable processes in Archae...

journal_title：Molecular microbiology

authors： Chaban B,Voisin S,Kelly J,Logan SM,Jarrell KF

更新日期：2006-07-01 00:00:00

abstract：:Many fungi undergo a morphological transition to filamentous growth in response to limiting nutrient conditions. Constitutively elongated Saccharomyces cerevisiae mutants (elm) have been isolated; the ELM1 gene encodes a putative serine/threonine protein kinase. A novel allele, elm1-15, has been isolated in an S288C-d...

journal_title：Molecular microbiology

authors： Garrett JM

更新日期：1997-11-01 00:00:00

abstract：:Enhancement of enzyme thermostability by protein engineering gives us information about the thermostabilization mechanism as well as advantages for industrial use of enzymes. In this study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase complex (cellulosome) from Clostridium cell...

journal_title：Molecular microbiology

authors： Murashima K,Kosugi A,Doi RH

更新日期：2002-08-01 00:00:00

abstract：:Malaria parasites scavenge nutrients from their host but also harbour enzymatic pathways for de novo macromolecule synthesis. One such pathway is apicoplast-targeted type II fatty acid synthesis, which is essential for late liver-stage development in rodent malaria. It is likely that fatty acids synthesized in the api...

journal_title：Molecular microbiology

authors： Lindner SE,Sartain MJ,Hayes K,Harupa A,Moritz RL,Kappe SH,Vaughan AM

更新日期：2014-02-01 00:00:00

abstract：:LamB glycoporin has an important general role in carbohydrate uptake during growth at low extracellular sugar concentrations. lamB and mal regulon induction during glucose starvation and glucose-limited continuous culture was investigated using lacZ fusions. A low-level burst of lamB induction occurred upon entry into...

journal_title：Molecular microbiology

authors： Notley L,Ferenci T

更新日期：1995-04-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the transcription factors Gln3p and Nil1p of the GATA family play a determinant role in expression of genes that are subject to nitrogen catabolite repression. Here we report the isolation of a new yeast mutant, gan1-1, exhibiting dramatically decreased NAD-linked glutamate dehydrogenase (...

journal_title：Molecular microbiology

authors： Soussi-Boudekou S,André B

更新日期：1999-02-01 00:00:00