RecA-mediated excision repair: a novel mechanism for repairing DNA lesions at sites of arrested DNA synthesis.

Abstract:

:In Escherichia coli, bulky DNA lesions are repaired primarily by nucleotide excision repair (NER). Unrepaired lesions encountered by DNA polymerase at the replication fork create a blockage which may be relieved through RecF-dependent recombination. We have designed an assay to monitor the different mechanisms through which a DNA polymerase blocked by a single AAF lesion may be rescued by homologous double-stranded DNA sequences. Monomodified single-stranded plasmids exhibit low survival in non-SOS induced E. coli cells; we show here that the presence of a homologous sequence enhances the survival of the damaged plasmid more than 10-fold in a RecA-dependent way. Remarkably, in an NER proficient strain, 80% of the surviving colonies result from the UvrA-dependent repair of the AAF lesion in a mechanism absolutely requiring RecA and RecF activity, while the remaining 20% of the surviving colonies result from homologous recombination mechanisms. These results uncover a novel mechanism - RecA-mediated excision repair - in which RecA-dependent pairing of the mono-modified single-stranded template with a complementary sequence allows its repair by the UvrABC excinuclease.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Bichara M,Pinet I,Lambert IB,Fuchs RP

doi

10.1111/j.1365-2958.2007.05790.x

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

218-29

issue

1

eissn

0950-382X

issn

1365-2958

pii

MMI5790

journal_volume

65

pub_type

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