FliL is essential for swarming: motor rotation in absence of FliL fractures the flagellar rod in swarmer cells of Salmonella enterica.

abstract：:The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subje...

journal_title：Molecular microbiology

authors： Libby RT,Gallant JA

更新日期：1991-05-01 00:00:00

abstract：:Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...

journal_title：Molecular microbiology

authors： Peters NT,Morlot C,Yang DC,Uehara T,Vernet T,Bernhardt TG

更新日期：2013-08-01 00:00:00

abstract：:Osmoregulatory mutants of Saccharomyces cerevisiae with a defect in their capacity to readjust the cell volume/buoyant density after osmotically induced dehydration were enriched by density gradient centrifugation. Colonies derived from cells that remained dense after dehydration were screened for sensitivity to high ...

journal_title：Molecular microbiology

authors： Larsson K,Ansell R,Eriksson P,Adler L

更新日期：1993-12-01 00:00:00

abstract：:N-linked protein glycosylation occurs in all three branches of life, eukaryotes, bacteria and archaea. The simplest system is that of the bacterium, Campylobacter jejuni, in which a heptasaccharide glycan is added to multiple proteins from a single lipid carrier molecule. In the eukaryotic system a conserved tetradeca...

journal_title：Molecular microbiology

authors： Guerry P

更新日期：2011-09-01 00:00:00

abstract：:The formation of biofilm results in a major lifestyle switch that is thought to affect the expression of multiple genes and operons. We used DNA arrays to study the global effect of biofilm formation on gene expression in mature Escherichia coli K-12 biofilm. We show that, when biofilm is compared with the exponential...

journal_title：Molecular microbiology

authors： Beloin C,Valle J,Latour-Lambert P,Faure P,Kzreminski M,Balestrino D,Haagensen JA,Molin S,Prensier G,Arbeille B,Ghigo JM

更新日期：2004-02-01 00:00:00

abstract：:Gram-negative bacteria, mitochondria, and chloroplasts all possess an outer membrane populated with a host of β-barrel outer-membrane proteins (βOMPs). These βOMPs play crucial roles in maintaining viability of their hosts, and therefore, it is essential to understand the biogenesis of this class of membrane proteins....

journal_title：Molecular microbiology

authors： Lundquist K,Billings E,Bi M,Wellnitz J,Noinaj N

更新日期：2020-12-13 00:00:00

abstract：:Mycoplasma hyopneumoniae induces respiratory disease in swine by colonizing cilia causing ciliostasis, cilial loss and epithelial cell death. Heparin binds to M. hyopneumoniae cells in a dose-dependent manner and blocks its ability to adhere to porcine cilia. We show here that Mhp493 (P216), a paralogue of the cilium ...

journal_title：Molecular microbiology

authors： Wilton J,Jenkins C,Cordwell SJ,Falconer L,Minion FC,Oneal DC,Djordjevic MA,Connolly A,Barchia I,Walker MJ,Djordjevic SP

更新日期：2009-02-01 00:00:00

abstract：:Pseudomonas aeruginosa, an opportunistic human pathogen, is a major causative agent of mortality and morbidity in immunocompromised individuals and those with cystic fibrosis (CF). In CF patients, the secretion of abnormally high amounts of mucus into the airways contributes to their susceptibility to infection by P. ...

journal_title：Molecular microbiology

authors： Wang J,Lory S,Ramphal R,Jin S

更新日期：1996-12-01 00:00:00

abstract：:A strategy was developed to mutate and genetically identify exported proteins in Streptococcus pneumoniae. Vectors were created and used to screen pneumococcal DNA in Escherichia coli and S. pneumoniae for translational gene fusions to alkaline phosphatase (PhoA). Twenty five PhoA+ pneumococcal mutants were isolated a...

journal_title：Molecular microbiology

authors： Pearce BJ,Yin YB,Masure HR

更新日期：1993-09-01 00:00:00

abstract：:Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here,...

journal_title：Molecular microbiology

authors： Davis R,Écija-Conesa A,Gallego-Jara J,de Diego T,Filippova EV,Kuffel G,Anderson WF,Gibson BW,Schilling B,Canovas M,Wolfe AJ

更新日期：2018-01-01 00:00:00

abstract：:We report the construction and the expression in Escherichia coli of three different fusion genes encoding the extended human IgG3 hinge region (Hi) fused in-phase to the C-terminal end of bacterial TEM1 beta-lactamase (Bla). In the first fusion gene blahi, TEM1 beta-lactamase (Bla). In the first fusion gene blahi, th...

journal_title：Molecular microbiology

authors： De Sutter K,Remaut E,Fiers W

更新日期：1992-08-01 00:00:00

abstract：:Bacteria of Shigella spp. are responsible for shigellosis in humans. They use a type III secretion (TTS) system encoded by a 200 kb virulence plasmid to enter epithelial cells and trigger apoptosis in macrophages. This TTS system comprises a secretion apparatus, translocators and effectors that transit through this ap...

journal_title：Molecular microbiology

authors： Penno C,Sansonetti P,Parsot C

更新日期：2005-04-01 00:00:00

abstract：:The bacteriocin pediocin PA-1 operon of Pediococcus acidilactici PAC1.0 encompasses four genes: pedA, pedB, pedC and pedD. Transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of pedA. The major transcript comprises pedA, pedB, and pe...

journal_title：Molecular microbiology

authors： Venema K,Kok J,Marugg JD,Toonen MY,Ledeboer AM,Venema G,Chikindas ML

更新日期：1995-08-01 00:00:00

abstract：:Bordetella pertussis regulates expression of its virulence factors such as pertussis toxin (Ptx) via the bvg locus, which encodes a two-component system composed of a sensor protein, BvgS, and a transcription activator, BvgA. We used a ptx-lac fusion on the B. pertussis chromosome to analyse promoter activation by alt...

journal_title：Molecular microbiology

authors： Marques RR,Carbonetti NH

更新日期：1997-06-01 00:00:00

abstract：:For malaria to be transmitted, the Plasmodium falciparum parasite must invade an erythrocyte and undergo gametocytogenesis. When mature intraerythrocytic gametocytes are taken up in a blood meal by a mosquito they emerge as gametes and, once fertilized, continue to differentiate into infectious sporozoites. One of the...

journal_title：Molecular microbiology

authors： Eksi S,Stump A,Fanning SL,Shenouda MI,Fujioka H,Williamson KC

更新日期：2002-06-01 00:00:00

abstract：:The development of new drugs for treating Pneumocystis carinii infections in AIDS patients is hampered by the lack of long-term culture systems, and by our generally limited knowledge of this organism. Recently, however, we observed significant activity of various benzimidazoles against growth of this organism in shor...

journal_title：Molecular microbiology

authors： Edlind TD,Bartlett MS,Weinberg GA,Prah GN,Smith JW

更新日期：1992-11-01 00:00:00

abstract：:The important human pathogen Streptococcus pyogenes (the group A streptococcus or GAS) causes diseases ranging from mild, self-limiting pharyngitis to severe invasive infections. Regulation of the expression of GAS genes in response to specific environmental differences within the host is probably key in determining t...

journal_title：Molecular microbiology

authors： Opdyke JA,Scott JR,Moran CP Jr

更新日期：2001-10-01 00:00:00

abstract：:On the basis of the published N-terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein-processing site, was shown ...

journal_title：Molecular microbiology

authors： Ehlert K,Höltje JV,Templin MF

更新日期：1995-05-01 00:00:00

abstract：:The molecular chaperone Hsp90 is a regulatory component of some key signalling proteins in the cytosol of eukaryotic cells. For some of these functions, its interaction with co-chaperones is required. Limited proteolysis defined stable folded units of Hsp90. Both an N-terminal (N210) and a C-terminal (262C) fragment i...

journal_title：Molecular microbiology

authors： Scheibel T,Weikl T,Rimerman R,Smith D,Lindquist S,Buchner J

更新日期：1999-11-01 00:00:00

abstract：:The cGvpE protein of Halobacterium salinarum PHH4 has been identified as transcriptional activator for the promoter of the c-gvpA gene encoding the major gas vesicle structural protein cGvpA. Molecular modelling of the carboxy-terminal region of cGvpE suggests that this protein resembles a basic leucine-zipper protein...

journal_title：Molecular microbiology

authors： Plösser P,Pfeifer F

更新日期：2002-07-01 00:00:00

abstract：:Metabolic adaptations are critical to the ability of bacterial pathogens to grow within host cells and are normally preceded by sensing of host-specific metabolic signals, which in turn can influence the pathogen's virulence state. Previously, we reported that the intracellular bacterial pathogen Listeria monocytogene...

journal_title：Molecular microbiology

authors： Lobel L,Sigal N,Borovok I,Belitsky BR,Sonenshein AL,Herskovits AA

更新日期：2015-02-01 00:00:00

abstract：:In all eukaryotic organisms, messenger RNA (mRNA) is synthesized in the nucleus and then exported to the cytoplasm for translation. The export reaction requires the concerted action of a large number of protein components, including a set of shuttle proteins that can exit and re-enter the nucleus through the nuclear p...

journal_title：Molecular microbiology

authors： Gratzer S,Beilharz T,Beddoe T,Henry MF,Lithgow T

更新日期：2000-03-01 00:00:00

abstract：:Glutaredoxins and thioredoxins are small heat-stable oxidoreductases that have been conserved throughout evolution. The yeast Saccharomyces cerevisiae contains two gene pairs encoding cytoplasmic glutaredoxins (GRX1, GRX2) and thioredoxins (TRX1, TRX2). We report here that the quadruple trx1 trx2 grx1 grx2 mutant is i...

journal_title：Molecular microbiology

authors： Draculic T,Dawes IW,Grant CM

更新日期：2000-06-01 00:00:00

abstract：:The psaBCA locus of Streptococcus pneumoniae encodes a putative ABC Mn2+-permease complex. Downstream of the operon is psaD, which may be co-transcribed and encodes a thiol peroxidase. Previously, there has been discordance concerning the phenotypic impact of mutations in the psa locus, resolution of which has been co...

journal_title：Molecular microbiology

authors： McAllister LJ,Tseng HJ,Ogunniyi AD,Jennings MP,McEwan AG,Paton JC

更新日期：2004-08-01 00:00:00

abstract：:To investigate the possible role of accessory subunits of Escherichia coli DNA polymerase III holoenzyme (HE) in determining chromosomal replication fidelity, we have investigated the role of the dnaX gene. This gene encodes both the tau and gamma subunits of HE, which play a central role in the organization and funct...

journal_title：Molecular microbiology

authors： Pham PT,Zhao W,Schaaper RM

更新日期：2006-02-01 00:00:00

abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...

journal_title：Molecular microbiology

authors： Simser JA,Rahman MS,Dreher-Lesnick SM,Azad AF

更新日期：2005-10-01 00:00:00

abstract：:Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or...

journal_title：Molecular microbiology

authors： Rozalski A,Brade L,Kosma P,Moxon R,Kusumoto S,Brade H

更新日期：1997-02-01 00:00:00

abstract：:TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

journal_title：Molecular microbiology

authors： Larsen RA,Wood GE,Postle K

更新日期：1993-12-01 00:00:00

abstract：:Summary Successful malaria transmission from the mosquito vector to the mammalian host depends crucially on active sporozoite motility. Sporozoite locomotion and host cell invasion are driven by the parasite's own actin/myosin motor. A unique feature of this motor machinery is the presence of very short subpellicular ...

journal_title：Molecular microbiology

authors： Ganter M,Schüler H,Matuschewski K

更新日期：2009-12-01 00:00:00

abstract：:The interactions between bacterial pathogens and their eukaryotic hosts are vital in determining the outcome of infections. Bacterial pathogens employ molecular sensors to detect and facilitate adaptation to changes in their niche. The sensing of these extracellular signals enables the pathogen to navigate within mamm...

journal_title：Molecular microbiology

authors： Karavolos MH,Winzer K,Williams P,Khan CM

更新日期：2013-02-01 00:00:00