Structural and genetic analysis of the bvg locus in Bordetella species.

abstract：:Phytopathogenic bacteria possess a large number of genes that allow them to grow and cause disease on plants. Many of these genes should be induced when the bacteria come in contact with plant tissue. We used a modified in vivo expression technology (IVET) approach to identify genes from the plant pathogen Pseudomonas...

journal_title：Molecular microbiology

authors： Boch J,Joardar V,Gao L,Robertson TL,Lim M,Kunkel BN

更新日期：2002-04-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) consists of a set of cytoplasmic energy-coupling proteins and various integral membrane permeases/sugar phosphotransferases, each specific for a different sugar. We have conducted biochemical analyses of three PTS permeases (enzymes II), the g...

journal_title：Molecular microbiology

authors： Aboulwafa M,Saier M Jr

更新日期：2003-04-01 00:00:00

abstract：:In Escherichia coli, the SeqA protein is known as a negative regulator of chromosome replication. This protein is also suggested to have a role in chromosome organization. SeqA preferentially binds to hemi-methylated DNA and is by immunofluorescence microscopy seen as foci situated at the replication factories. Loss o...

journal_title：Molecular microbiology

authors： Klungsøyr HK,Skarstad K

更新日期：2004-10-01 00:00:00

abstract：:PII-like signalling molecules are trimeric proteins composed of 12-13 kDa polypeptides encoded by the glnB gene family. Heterologous expression of a cyanobacterial glnB gene in Escherichia coli leads to an inactivation of E. coli's own PII signalling system. In the present work, we show that this effect is caused by t...

journal_title：Molecular microbiology

authors： Forchhammer K,Hedler A,Strobel H,Weiss V

更新日期：1999-07-01 00:00:00

abstract：:We screened the putative rap-phr (response regulator aspartyl-phosphate phosphatase-phosphatase regulator) systems identified in the Bacillus subtilis genome for a rap gene that affects aprE (alkaline protease gene) expression by using a multicopy plasmid. We found that rapG was involved in the regulation of aprE, whi...

journal_title：Molecular microbiology

authors： Ogura M,Shimane K,Asai K,Ogasawara N,Tanaka T

更新日期：2003-09-01 00:00:00

abstract：:In cell-free Yersinia pseudotuberculosis culture supernatants, we have chemically characterized three N-acyl homoserine lactone (AHL) molecules, N-octanoyl homoserine lactone (C8-HSL), N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6-HSL) and N-hexanoyl homoserine lactone (C6-HSL). We have identified, cloned and sequence...

journal_title：Molecular microbiology

authors： Atkinson S,Throup JP,Stewart GS,Williams P

更新日期：1999-09-01 00:00:00

abstract：:The involvement of signal transduction in the repair of radiation-induced damage to DNA has been known in eukaryotes but remains understudied in bacteria. This article for the first time demonstrates a role for the periplasmic lipoprotein (YfgL) with protein kinase activity transducing a signal for DNA strand break re...

journal_title：Molecular microbiology

authors： Khairnar NP,Kamble VA,Mangoli SH,Apte SK,Misra HS

更新日期：2007-07-01 00:00:00

abstract：:The carboxyl-terminal region of diphtheria toxin (DT) has been analysed in order to determine regions of receptor recognition. Biochemical cleavage of the toxin with hydroxylamine (HA) was used to generate the peptides HA9DT (residues 454-535), HA6DT (residues 482-535), and HA3DT (residues 454-481). Characterization o...

journal_title：Molecular microbiology

authors： Rolf JM,Eidels L

更新日期：1993-02-01 00:00:00

abstract：:Fourteen Rhodobacter capsulatus mutants unable to grow with xanthine as sole nitrogen source were isolated by random Tn5 mutagenesis. Five of these Tn5 insertions were mapped within two adjacent chromosomal EcoRI fragments hybridizing to oligonucleotides synthesized according to conserved amino acid sequences of eukar...

journal_title：Molecular microbiology

authors： Leimkühler S,Kern M,Solomon PS,McEwan AG,Schwarz G,Mendel RR,Klipp W

更新日期：1998-02-01 00:00:00

abstract：:We identified interactions between the conserved bacterial replication initiator and transcription factor DnaA and the nucleoid-associated protein Rok of Bacillus subtilis. DnaA binds directly to clusters of DnaA boxes at the origin of replication and elsewhere, including the promoters of several DnaA-regulated genes....

journal_title：Molecular microbiology

authors： Seid CA,Smith JL,Grossman AD

更新日期：2017-03-01 00:00:00

abstract：:Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, A...

journal_title：Molecular microbiology

authors： Gourlay SC,Colloms SD

更新日期：2004-04-01 00:00:00

abstract：:Translating ribosomes require elongation factor P (EF-P) to incorporate consecutive prolines (XPPX) into nascent peptide chains. The proteome of Corynebacterium glutamicum ATCC 13032 contains a total of 1,468 XPPX motifs, many of which are found in proteins involved in primary and secondary metabolism. We show here th...

journal_title：Molecular microbiology

authors： Pinheiro B,Petrov DP,Guo L,Martins GB,Bramkamp M,Jung K

更新日期：2020-10-04 00:00:00

abstract：:Expression of Escherichia coli hupA and hupB, the structural genes encoding the most abundant nucleoid-associated proteins HUalpha and HUbeta has been studied during cold shock. This article demonstrates that: (i) transcriptional expression of hupA is blocked following a sudden temperature downshift (from 37 degrees C...

journal_title：Molecular microbiology

authors： Giangrossi M,Giuliodori AM,Gualerzi CO,Pon CL

更新日期：2002-04-01 00:00:00

abstract：:Strains of Neisseria meningitidis express one of two porin proteins. These porins have been identified as the class 2 and class 3 proteins, and express serotype-specific epitopes. The gene for the class 3 protein was amplified by the polymerase chain reaction from the DNA of a serotype 4 strain as a 1025 bp fragment. ...

journal_title：Molecular microbiology

authors： Zapata GA,Vann WF,Rubinstein Y,Frasch CE

更新日期：1992-12-01 00:00:00

abstract：:On the basis of the published N-terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein-processing site, was shown ...

journal_title：Molecular microbiology

authors： Ehlert K,Höltje JV,Templin MF

更新日期：1995-05-01 00:00:00

abstract：:Many filamentous cyanobacteria are capable of gliding motility by an undefined mechanism. Within the heterocyst-forming clades, some strains, such as the Nostoc spp. and Fisherella spp., are motile only as specialized filaments termed hormogonia. Here we report on the phenotype of inactivation of a methyl-accepting ch...

journal_title：Molecular microbiology

authors： Risser DD,Meeks JC

更新日期：2013-02-01 00:00:00

abstract：:The assembly of proteins that display complementary activities into supramolecular intra- and extracellular complexes is central to cellular function. One such nanomachine of considerable biological and industrial significance is the plant cell wall degrading apparatus of anaerobic bacteria termed the cellulosome. The...

journal_title：Molecular microbiology

authors： Gilbert HJ

更新日期：2007-03-01 00:00:00

abstract：:In conditions of halted or limited genome replication, like those experienced in sporulating cells of Bacillus subtilis, a more immediate detriment caused by DNA damage is altering the transcriptional programme that drives this developmental process. Here, we report that mfd, which encodes a conserved bacterial protei...

journal_title：Molecular microbiology

authors： Ramírez-Guadiana FH,Del Carmen Barajas-Ornelas R,Ayala-García VM,Yasbin RE,Robleto E,Pedraza-Reyes M

更新日期：2013-12-01 00:00:00

abstract：:Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type...

journal_title：Molecular microbiology

authors： Schmoll M,Seibel C,Tisch D,Dorrer M,Kubicek CP

更新日期：2010-09-01 00:00:00

abstract：:Type VI secretion is critical for Vibrio cholerae to successfully combat phagocytic eukaryotes and to survive in the presence of competing bacterial species. V. cholerae type VI secretion system genes are encoded in one large and two small clusters. In V. cholerae, type VI secretion is controlled by quorum sensing, th...

journal_title：Molecular microbiology

authors： Shao Y,Bassler BL

更新日期：2014-06-01 00:00:00

abstract：:Borrelia hermsii, an agent of relapsing fever, avoids the host's immune response by means of multiphasic antigenic variation. Serotype specificity is determined by variable antigens called the Vmp lipoproteins. Through recombination between linear plasmids a formerly silent vmp gene replaces another vmp gene at a telo...

journal_title：Molecular microbiology

authors： Barbour AG,Burman N,Carter CJ,Kitten T,Bergström S

更新日期：1991-02-01 00:00:00

abstract：:srfA is a locus required for the production of the lipopeptide antibiotic surfactin. This locus is also necessary for efficient sporulation and competence development. Mutations in the 5' portion of the srfA operon affect all three of these processes, whereas mutations in the 3' portion of srfA only affect sporulation...

journal_title：Molecular microbiology

authors： van Sinderen D,Galli G,Cosmina P,de Ferra F,Withoff S,Venema G,Grandi G

更新日期：1993-05-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active sta...

journal_title：Molecular microbiology

authors： Nakamura S,Hanaizumi Y,Morimoto YV,Inoue Y,Erhardt M,Minamino T,Namba K

更新日期：2020-04-01 00:00:00

abstract：:Previous evidence showed that b- and a-type flagellins of Pseudomonas aeruginosa are modified in vivo by phosphorylation at tyrosine. This research was designed to demonstrate phosphorylation of flagellin at tyrosine in vitro. Evidence presented showed that flagellin is labelled by [gamma-32P]-ATP, but not by [alpha-3...

journal_title：Molecular microbiology

authors： South SL,Nichols R,Montie TC

更新日期：1994-06-01 00:00:00

abstract：:DNA processing reactions often involve multiple components acting in concert to achieve the desired outcome. However, it is usually difficult to know how the components communicate and cooperate to orchestrate an ordered series of events. We address this question in the context of the Tn 10 transposition reaction, in ...

journal_title：Molecular microbiology

authors： Liu D,Sewitz S,Crellin P,Chalmers R

更新日期：2006-12-01 00:00:00

abstract：:OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth con...

journal_title：Molecular microbiology

authors： Feng X,Walthers D,Oropeza R,Kenney LJ

更新日期：2004-11-01 00:00:00

abstract：:Bacillus subtilis responds to signals of environmental and metabolic stress by inducing over 40 general stress genes under the control of the sigma B transcription factor. sigma B activity is regulated post-translationally by a multi-component network composed of two coupled partner-switching modules, RsbX-RsbS-RsbT a...

journal_title：Molecular microbiology

authors： Akbar S,Kang CM,Gaidenko TA,Price CW

更新日期：1997-05-01 00:00:00

abstract：:Iron-sulfur (Fe-S) clusters are prosthetic groups on proteins that function in a range of enzymatic and electron transfer reactions. Fe-S cluster synthesis is essential for the survival of all eukaryotes. Independent Fe-S cluster biosynthesis pathways occur in the mitochondrion, plastid, and cytosolic compartments of ...

journal_title：Molecular microbiology

authors： Aw YTV,Seidi A,Hayward JA,Lee J,Makota FV,Rug M,van Dooren GG

更新日期：2020-11-22 00:00:00

abstract：:The Streptomyces wild-type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5' ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0...

journal_title：Molecular microbiology

authors： Volff JN,Altenbuchner J

更新日期：1998-01-01 00:00:00