Transcription of a Rhizobium leguminosarum biovar phaseoli gene needed for melanin synthesis is activated by nifA of Rhizobium and Klebsiella pneumoniae.

abstract：:In vitro, the sigma(s) subunit of RNA polymerase (RNAP), RpoS, recognizes nearly identical -35 and -10 promoter consensus sequences as the vegetative sigma70. In vivo, promoter selectivity of RNAP holoenzyme containing either sigma(s) (Esigma(s)) or sigma70 (Esigma70) seems to be achieved by the differential ability o...

journal_title：Molecular microbiology

authors： Typas A,Hengge R

更新日期：2006-02-01 00:00:00

abstract：:The cyanobacterium Synechocystis sp. PCC 6803 expresses four different FtsH protease subunits (FtsH1-4) that assemble into specific homo- and heterocomplexes. The FtsH2/FtsH3 complex is involved in photoprotection but the physiological roles of the other complexes, notably the essential FtsH1/FtsH3 complex, remain unc...

journal_title：Molecular microbiology

authors： Krynická V,Tichý M,Krafl J,Yu J,Kaňa R,Boehm M,Nixon PJ,Komenda J

更新日期：2014-11-01 00:00:00

abstract：:Each Pseudomonas aeruginosa cell localizes two types of motility structures, a single flagellum and one or two clusters of type IV pili, to the cell poles. Previous studies suggested that these motility structures arrive at the pole through distinct mechanisms. Here we performed a swimming motility screen to identify ...

journal_title：Molecular microbiology

authors： Cowles KN,Moser TS,Siryaporn A,Nyakudarika N,Dixon W,Turner JJ,Gitai Z

更新日期：2013-12-01 00:00:00

abstract：:The porin oprE gene of Pseudomonas aeruginosa PAO1 was isolated. Its nucleotide sequence indicated that the structural gene of 1383 nucleotide residues encodes a precursor consisting of 460 amino acid residues with a signal peptide of 29 amino acid residues, which was confirmed by the N-terminal 23-amino-acid sequence...

journal_title：Molecular microbiology

authors： Yamano Y,Nishikawa T,Komatsu Y

更新日期：1993-05-01 00:00:00

abstract：:Numerous eukaryotes have developed specific metabolic traits that are not present in extensively studied model organisms. For instance, the procyclic insect form of Trypanosoma brucei, a parasite responsible for sleeping sickness in its mammalian-specific bloodstream form, metabolizes glucose into excreted succinate a...

journal_title：Molecular microbiology

authors： Bringaud F,Biran M,Millerioux Y,Wargnies M,Allmann S,Mazet M

更新日期：2015-06-01 00:00:00

abstract：:The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiom...

journal_title：Molecular microbiology

authors： Hardy SJ,Christodoulides M,Weller RO,Heckels JE

更新日期：2000-05-01 00:00:00

abstract：:Bactericidal/permeability-increasing protein (BPI) of neutrophils is a lipopolysaccharide (LPS)-binding antibacterial protein with specificity for Gram negative bacteria. BPI binding to the bacterial surface rapidly triggers potentially reversible bacterial growth inhibition and alterations of the outer membrane and, ...

journal_title：Molecular microbiology

authors： Prohinar P,Forst SA,Reed D,Mandic-Mulec I,Weiss J

更新日期：2002-03-01 00:00:00

abstract：:Transcription of flagellar genes in Caulobacter crecentus is programmed to occur during the predivisional stage of the cell cycle. The mechanism of activation of Class II flagellar genes, the highest identified genes in the Caulobacter flagellar hierarchy, is unknown. As a step toward understanding this process, we ha...

journal_title：Molecular microbiology

authors： Stephens CM,Shapiro L

更新日期：1993-09-01 00:00:00

abstract：:When Escherichia coli is exposed to redox-cycling drugs, its SoxR transcription factor is activated by oxidation of its [2Fe-2S] cluster. In aerobic cells these drugs generate superoxide, and because superoxide dismutase (SOD) is a member of the SoxRS regulon, superoxide was initially thought to be the activator of So...

journal_title：Molecular microbiology

authors： Gu M,Imlay JA

更新日期：2011-03-01 00:00:00

abstract：:In their natural habitats, microorganisms are often exposed to osmolality changes in the environment. The osmotic stress must be sensed and converted into an activity change of specific enzymes and transport proteins and/or it must trigger their synthesis such that the osmotic imbalance can be rapidly restored. On the...

journal_title：Molecular microbiology

authors： Poolman B,Glaasker E

更新日期：1998-07-01 00:00:00

abstract：:In most soft-rotting Erwinia spp., including E. carotovora subsp. carotovora strain 71 (Ecc71), production of the plant cell wall degrading enzyme pectin lyase (Pnl) is activated by DNA-damaging agents such as mitomycin C (MC). Induction of Pnl production in Ecc71 requires a functional recA gene and the rdg locus. DNA...

journal_title：Molecular microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-12-01 00:00:00

abstract：:Although SsrA(tmRNA)-mediated trans-translation is thought to maintain the translation capacity of bacterial cells by rescuing ribosomes stalled on messenger RNA lacking an in-frame stop codon, single disruption of ssrA does not crucially hamper growth of Escherichia coli. Here, we identified YhdL (renamed ArfA for al...

journal_title：Molecular microbiology

authors： Chadani Y,Ono K,Ozawa S,Takahashi Y,Takai K,Nanamiya H,Tozawa Y,Kutsukake K,Abo T

更新日期：2010-11-01 00:00:00

abstract：:The GATA factor AreA is a wide-domain regulator in Aspergillus nidulans with transcriptional activation and chromatin remodelling functions. AreA interacts with the nitrate-specific Zn(2)-C(6) cluster protein NirA and both proteins cooperate to synergistically activate nitrate-responsive genes. We have previously esta...

journal_title：Molecular microbiology

authors： Berger H,Pachlinger R,Morozov I,Goller S,Narendja F,Caddick M,Strauss J

更新日期：2006-01-01 00:00:00

abstract：:Oligosaccharides derived from cell wall of fungal pathogens induce host primary immune responses. To understand fungal strategies circumventing the host plant immune responses, cell wall polysaccharide localization was investigated using fluorescent labels during infectious structure differentiation in the rice blast ...

journal_title：Molecular microbiology

authors： Fujikawa T,Kuga Y,Yano S,Yoshimi A,Tachiki T,Abe K,Nishimura M

更新日期：2009-08-01 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs) are persistent and toxic environmental pollutants that accumulate in anoxic habitats. With the exception of naphthalene, nothing is known about the microbial degradation of PAH in these environments. The challenge that must be met in anaerobic PAH degradation is the destabilizat...

journal_title：Molecular microbiology

authors： Sawers RG

更新日期：2015-01-01 00:00:00

abstract：:Cpmk2, encoding a mitogen-activated protein (MAP) kinase from the ascomycete Claviceps purpurea, is an orthologue of SLT2 from Saccharomyces cerevisiae, the first isolated from a biotrophic, non-appressorium-forming pathogen. Deletion mutants obtained by a gene replacement approach show impaired vegetative properties ...

journal_title：Molecular microbiology

authors： Mey G,Held K,Scheffer J,Tenberge KB,Tudzynski P

更新日期：2002-10-01 00:00:00

abstract：:9 alpha-Hydroxylation of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial steroid catabolism. Very limited knowledge is presently available on the KSH enzyme. Here, we report for the first time the identification and ...

journal_title：Molecular microbiology

authors： van der Geize R,Hessels GI,van Gerwen R,van der Meijden P,Dijkhuizen L

更新日期：2002-08-01 00:00:00

abstract：:The lipopolysaccharide (LPS) of H. influenzae is highly variable. Much of the structural diversity is derived from phase variation, or high frequency on-off switching, of molecules attached during LPS biosynthesis. In this study, we examined the dynamics of LPS phase variation following exposure to human serum as a so...

journal_title：Molecular microbiology

authors： Clark SE,Eichelberger KR,Weiser JN

更新日期：2013-05-01 00:00:00

abstract：:Copy-number mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B (MLS) resistance plasmid pT48 were isolated by their resistance to the non-inducing macrolide, tylosin. One mutant plasmid, pcopD3, showed a three- to five-fold cis-dominant increase in copy number, and nucleotide sequence analysis reve...

journal_title：Molecular microbiology

authors： Catchpole I,Dyke KG

更新日期：1991-04-01 00:00:00

abstract：:Salmonella typhimurium responds to a variety of environmental stresses by accumulating the alternative sigma factor sigmaS. The repertoire of sigmaS -dependent genes that are subsequently expressed confers tolerance to a variety of potentially lethal conditions including low pH and stationary phase. The mechanism(s) r...

journal_title：Molecular microbiology

authors： Webb C,Moreno M,Wilmes-Riesenberg M,Curtiss R 3rd,Foster JW

更新日期：1999-10-01 00:00:00

abstract：:In Escherichia coli, RecA protein regulates the DNA damage-inducible survival-enhancing SOS response. Mutant allele recA730, which causes constitutive SOS expression, is lethal at high temperatures in B/r, a derivative of wild-type B, but not in K-12 or in certain B/r--K-12 hybrids. We present evidence that killing is...

journal_title：Molecular microbiology

authors： Kirchner J,Lim D,Witkin EM,Garvey N,Roegner-Maniscalco V

更新日期：1992-10-01 00:00:00

abstract：:Attaching and effacing (A/E) lesions and actin polymerization, the hallmark of enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC) and Citrobacter rodentium (CR) infections, are dependent on the effector Tir. Phosphorylation of Tir(EPEC/CR) Y474/1 leads to recruitment of Nck and neural Wiskott-A...

journal_title：Molecular microbiology

authors： Crepin VF,Girard F,Schüller S,Phillips AD,Mousnier A,Frankel G

更新日期：2010-01-01 00:00:00

abstract：:Bacterial protein secretion is catalysed by the SecYEG protein-conducting channel complexed with the SecA ATPase motor. To gain insight into the SecA-SecYEG interaction we used peptide arrays, thermodynamic quantification, mutagenesis and functional assays. Our data reveal that: (i) SecA binds with low affinity on sev...

journal_title：Molecular microbiology

authors： Karamanou S,Bariami V,Papanikou E,Kalodimos CG,Economou A

更新日期：2008-10-01 00:00:00

abstract：:Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation ...

journal_title：Molecular microbiology

authors： Dempsey WB,Fee BE

更新日期：1990-06-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:To identify Rhodobacter capsulatus nif genes necessary for the alternative nitrogenase, strains carrying defined mutations in 32 genes and open reading frames of nif region A, B or C were constructed. The ability of these mutants to grow on nitrogen-free medium with molybdenum (Nif phenotype) or in a nifHDK deletion b...

journal_title：Molecular microbiology

authors： Schüddekopf K,Hennecke S,Liese U,Kutsche M,Klipp W

更新日期：1993-05-01 00:00:00

abstract：:The secretion of numerous proteins during vegetative growth of Myxococcus xanthus, and the multicellular development cycle induced upon starvation of these bacteria, are partially interrelated in so far as mutants impaired in extracellular protein production are unable to undergo development. We have cloned and sequen...

journal_title：Molecular microbiology

authors： Laval-Favre K,Letouvet-Pawlak B,Friedrich T,Alexandre J,Guespin-Michel JF

更新日期：1997-03-01 00:00:00

abstract：:Strains of Rhizobium leguminosarum biovar viciae specifically make an abundant protein (Rhi) in free-living culture but not in bacteroids. Genes needed for Rhi synthesis are on a Sym plasmid and here we show that one of these genes, rhiA, is the structural gene that specifies this polypeptide. Transcription of rhiA re...

journal_title：Molecular microbiology

authors： Economou A,Hawkins FK,Downie JA,Johnston AW

更新日期：1989-01-01 00:00:00

abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

journal_title：Molecular microbiology

authors： Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

更新日期：2009-06-01 00:00:00

abstract：:CS1 pili are filamentous proteinaceous appendages found on many enterotoxigenic Escherichia coli (ETEC) strains isolated from human diarrhoeal disease. They are thought to effect colonization of the upper intestine by facilitating binding to human ileal epithelial cells. We have identified a gene, cooB, which lies dir...

journal_title：Molecular microbiology

authors： Scott JR,Wakefield JC,Russell PW,Orndorff PE,Froehlich BJ

更新日期：1992-02-01 00:00:00