Abstract:
:The porin oprE gene of Pseudomonas aeruginosa PAO1 was isolated. Its nucleotide sequence indicated that the structural gene of 1383 nucleotide residues encodes a precursor consisting of 460 amino acid residues with a signal peptide of 29 amino acid residues, which was confirmed by the N-terminal 23-amino-acid sequence and the reaction with anti-OprE polyclonal antiserum. Anaerobiosis induced OprE production at the transcription level. The transcription start site was determined to be 40 nucleotides upstream from the ATG initiation codon. The control region contained an appropriately situated E sigma 54 recognition site and the putative second half of an ANR box. The amino acid sequence of OprE had some clusters of sequence homologous with that of OprD of P. aeruginosa, which might be responsible for the outer membrane permeability of imipenem and basic amino acids.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Yamano Y,Nishikawa T,Komatsu Ydoi
10.1111/j.1365-2958.1993.tb01643.xsubject
Has Abstractpub_date
1993-05-01 00:00:00pages
993-1004issue
5eissn
0950-382Xissn
1365-2958journal_volume
8pub_type
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