Klebsiella pneumoniae strain K21: evidence for the rapid secretion of an unacylated form of pullulanase.

abstract：:A method of insertional mutagenesis for naturally transformable organisms has been adapted from Haemophilus influenzae and applied to the study of the pathogenesis of Campylobacter jejuni. A series of kanamycin-resistant insertional mutants of C. jejuni 81-176 has been generated and screened for loss of ability to inv...

journal_title：Molecular microbiology

authors： Yao R,Burr DH,Doig P,Trust TJ,Niu H,Guerry P

更新日期：1994-12-01 00:00:00

abstract：:Mycobacterium smegmatis mc2 155 has three genes (MSMEG_6383, furA1; MSMEG_3460, furA2; MSMEG_6253, furA3) encoding FurA (ferric-uptake regulator A) paralogs. Three FurA paralogs in M. smegmatis are functionally redundant and negatively regulate expression of a subset of genes involved in peroxide detoxification such a...

journal_title：Molecular microbiology

authors： Lee HN,Ji CJ,Lee HH,Park J,Seo YS,Lee JW,Oh JI

更新日期：2018-06-01 00:00:00

abstract：:The molecular chaperone Hsp90 is a regulatory component of some key signalling proteins in the cytosol of eukaryotic cells. For some of these functions, its interaction with co-chaperones is required. Limited proteolysis defined stable folded units of Hsp90. Both an N-terminal (N210) and a C-terminal (262C) fragment i...

journal_title：Molecular microbiology

authors： Scheibel T,Weikl T,Rimerman R,Smith D,Lindquist S,Buchner J

更新日期：1999-11-01 00:00:00

abstract：:We have measured repair of both of the major lesions induced by ultraviolet irradiation (cyclobutane pyrimidine dimers and 6-4 photoproducts) in wild-type Schizosaccharomyces pombe and in selected rad mutants, including mutants with deletions in genes from the main phenotypic groups. We find that rad13 delta, rad15 an...

journal_title：Molecular microbiology

authors： McCready S,Carr AM,Lehmann AR

更新日期：1993-11-01 00:00:00

abstract：:Two operons, comAB and comCDE, play a key role in the co-ordination of spontaneous competence development in cultures of Streptococcus pneumoniae. ComAB is required for export of the comC-encoded competence-stimulating peptide (CSP). Upon CSP binding, the histidine kinase ComD activates ComE, its cognate response regu...

journal_title：Molecular microbiology

authors： Martin B,Prudhomme M,Alloing G,Granadel C,Claverys JP

更新日期：2000-11-01 00:00:00

abstract：:The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active sta...

journal_title：Molecular microbiology

authors： Nakamura S,Hanaizumi Y,Morimoto YV,Inoue Y,Erhardt M,Minamino T,Namba K

更新日期：2020-04-01 00:00:00

abstract：:Reversible protein acetylation is established as a modification of major regulatory significance. In particular, histone acetylation regulates access to genetic information in eukaryotes. For example, class I and class II histone deacetylases are regulatory components of corepressor complexes involved in cell cycle pr...

journal_title：Molecular microbiology

authors： Ingram AK,Horn D

更新日期：2002-07-01 00:00:00

abstract：:The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription termin...

journal_title：Molecular microbiology

authors： Braun F,Hajnsdorf E,Régnier P

更新日期：1996-03-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae nuclear gene CYB2 encoding the mitochondrial enzyme L-(+)-lactate-cytochrome c oxidoreductase (EC 1.2.2.3) is subject to several strict metabolic controls at the transcriptional level: repression due to glucose fermentation, derepression by ethanol, induction by lactate and i...

journal_title：Molecular microbiology

authors： Ramil E,Agrimonti C,Shechter E,Gervais M,Guiard B

更新日期：2000-09-01 00:00:00

abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...

journal_title：Molecular microbiology

authors： Santos C,Ballesta JP

更新日期：2005-10-01 00:00:00

abstract：:A biochemical approach to identify proteins with high affinity for choline-containing pneumococcal cell walls has allowed the localization, cloning and sequencing of a gene (lytC ) coding for a protein that degrades the cell walls of Streptococcus pneumoniae. The lytC gene is 1506 bp long and encodes a protein (LytC) ...

journal_title：Molecular microbiology

authors： García P,Paz González M,García E,García JL,López R

更新日期：1999-07-01 00:00:00

abstract：:Clusters of regularly interspaced short palindromic repeats (CRISPRs) of Sulfolobus fall into three main families based on their repeats, leader regions, associated cas genes and putative recognition sequences on viruses and plasmids. Spacer sequence matches to different viruses and plasmids of the Sulfolobales reveal...

journal_title：Molecular microbiology

authors： Lillestøl RK,Shah SA,Brügger K,Redder P,Phan H,Christiansen J,Garrett RA

更新日期：2009-04-01 00:00:00

abstract：:Cell-cell communication via the production and detection of chemical signal molecules has been the focus of a great deal of research over the past decade. One class of chemical signals widely used by proteobacteria consists of N-acyl-homoserine lactones, which are synthesized by proteins related to LuxI of Vibrio fisc...

journal_title：Molecular microbiology

authors： Pappas KM,Weingart CL,Winans SC

更新日期：2004-08-01 00:00:00

abstract：:Electron microscopic studies have demonstrated that various gliding filamentous cyanobacteria have trichome surfaces with a common structural organization. They contain an S-layer attached to the outer membrane and an array of parallel fibrils on top of the S-layer. In all species studied, the helical arrangement of t...

journal_title：Molecular microbiology

authors： Hoiczyk E,Baumeister W

更新日期：1997-11-01 00:00:00

abstract：:The mitis group streptococci (MGS) are widespread in the oral cavity and are traditionally associated with oral health. However, these organisms have many attributes that contribute to the development of pathogenic oral communities. MGS adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment su...

journal_title：Molecular microbiology

authors： Whitmore SE,Lamont RJ

更新日期：2011-07-01 00:00:00

abstract：:Numerous eukaryotes have developed specific metabolic traits that are not present in extensively studied model organisms. For instance, the procyclic insect form of Trypanosoma brucei, a parasite responsible for sleeping sickness in its mammalian-specific bloodstream form, metabolizes glucose into excreted succinate a...

journal_title：Molecular microbiology

authors： Bringaud F,Biran M,Millerioux Y,Wargnies M,Allmann S,Mazet M

更新日期：2015-06-01 00:00:00

abstract：:Chromalveolates like the diatom Phaeodactylum tricornutum arose through the uptake of a red alga by a phagotrophic protist, a process termed secondary endosymbiosis. In consequence, the plastids are surrounded by two additional membranes compared with primary plastids. This plastid morphology poses additional barriers...

journal_title：Molecular microbiology

authors： Hempel F,Felsner G,Maier UG

更新日期：2010-05-01 00:00:00

abstract：:Members of the IS3 family of insertion sequences are found in a wide range of bacteria. At least 10 members of this family carry two major open reading frames: a small upstream frame (0 phase), and a longer downstream frame in the -1 phase. The downstream frame shows significant similarity at the amino acid level. A h...

journal_title：Molecular microbiology

authors： Fayet O,Ramond P,Polard P,Prère MF,Chandler M

更新日期：1990-10-01 00:00:00

abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

journal_title：Molecular microbiology

authors： Shakhnovich EA,Sturtevant D,Mekalanos JJ

更新日期：2007-12-01 00:00:00

abstract：:SpollE is an integral membrane protein that governs the establishment of cell-specific gene transcription during the process of sporulation in Bacillus subtilis. Synthesis of SpollE commences shortly after the onset of sporulation, after which the protein localizes at sites of potential cell division near both ends of...

journal_title：Molecular microbiology

authors： Levin PA,Losick R,Stragier P,Arigoni F

更新日期：1997-09-01 00:00:00

abstract：:Biofilm formation is a social behaviour that generates favourable conditions for sustained survival in the natural environment. For the Gram-positive bacterium Bacillus subtilis the process involves the differentiation of cell fate within an isogenic population and the production of communal goods that form the biofil...

journal_title：Molecular microbiology

authors： Cairns LS,Hobley L,Stanley-Wall NR

更新日期：2014-08-01 00:00:00

abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...

journal_title：Molecular microbiology

authors： Westermayer SA,Fritz G,Gutiérrez J,Megerle JA,Weißl MP,Schnetz K,Gerland U,Rädler JO

更新日期：2016-05-01 00:00:00

abstract：:Two-component signal transduction (TCST) pathways are regulatory systems that are highly homologous throughout the bacterial kingdom. Their established role in virulence and absence in vertebrates has made TCST an attractive target for therapeutic intervention. However, such systems have yet to yield success in the de...

journal_title：Molecular microbiology

authors： Hubbard JA,MacLachlan LK,King GW,Jones JJ,Fosberry AP

更新日期：2003-09-01 00:00:00

abstract：:The Myxococcus xanthus gene, pkn9, encodes a protein that contains significant homology with eukaryotic Ser/Thr protein kinases. The pkn9 gene was singled out of a previously identified family of kinase genes by amplification techniques that displayed differences in kinase gene expression during selected periods of th...

journal_title：Molecular microbiology

authors： Hanlon WA,Inouye M,Inouye S

更新日期：1997-02-01 00:00:00

abstract：:The interactions between pathogenic bacteria and the host need to be resolved at the molecular level in order to develop novel vaccines and drugs. We have previously identified strepadhesin, a novel glycoprotein-binding activity in Streptococcus pyogenes, which is regulated by Mga, a regulator of streptococcal virulen...

journal_title：Molecular microbiology

authors： Hytönen J,Haataja S,Gerlach D,Podbielski A,Finne J

更新日期：2001-01-01 00:00:00

abstract：:Ribosome-tethered chaperones that interact with nascent polypeptide chains have been identified in both prokaryotic and eukaryotic systems. However, these ribosome-associated chaperones share no sequence similarity: bacterial trigger factors (TF) form an independent protein family while the yeast machinery is Hsp70-ba...

journal_title：Molecular microbiology

authors： Rauch T,Hundley HA,Pfund C,Wegrzyn RD,Walter W,Kramer G,Kim SY,Craig EA,Deuerling E

更新日期：2005-07-01 00:00:00

abstract：:When fungi grow on plant or insect surfaces coated with wax polyesters that protect against pathogens, the fungi generally form aerial hyphae to contact the surfaces. Aerial structures such as hyphae and conidiophores are coated with hydrophobins, which are surface-active proteins involved in adhesion to hydrophobic s...

journal_title：Molecular microbiology

authors： Takahashi T,Maeda H,Yoneda S,Ohtaki S,Yamagata Y,Hasegawa F,Gomi K,Nakajima T,Abe K

更新日期：2005-09-01 00:00:00

abstract：:The recent development of specific probes for lipid molecules has led to the discovery of lipid domains in bacterial membranes, that is, of membrane areas differing in lipid composition. A view of the membrane as a patchwork is replacing the assumption of lipid homogeneity inherent in the fluid mosaic model of Singer ...

journal_title：Molecular microbiology

authors： Matsumoto K,Kusaka J,Nishibori A,Hara H

更新日期：2006-09-01 00:00:00

abstract：:The bioluminescent marine bacterium Vibrio harveyi controls light production (lux) by an elaborate quorum-sensing circuit. V. harveyi produces and responds to two different autoinducer signals (AI-1 and AI-2) to modulate the luciferase structural operon (luxCDABEGH) in response to changes in cell-population density. U...

journal_title：Molecular microbiology

authors： Lilley BN,Bassler BL

更新日期：2000-05-01 00:00:00

abstract：:All 21 of the Nudix hydrolase genes from the radiation-resistant organism Deinococcus radiodurans have been cloned into vectors under the control of T7 promoters and expressed as soluble proteins in Escherichia coli. Their sizes range from 9.8 kDa (91 amino acids) to 59 kDa (548 amino acids). Two novel proteins were i...

journal_title：Molecular microbiology

authors： Xu W,Shen J,Dunn CA,Desai S,Bessman MJ

更新日期：2001-01-01 00:00:00