Abstract:
:All 21 of the Nudix hydrolase genes from the radiation-resistant organism Deinococcus radiodurans have been cloned into vectors under the control of T7 promoters and expressed as soluble proteins in Escherichia coli. Their sizes range from 9.8 kDa (91 amino acids) to 59 kDa (548 amino acids). Two novel proteins were identified, each with two Nudix boxes in its primary structure, unique among all other known Nudix hydrolases. Extracts of each of the expressed proteins were assayed by a generalized procedure that measures the hydrolysis of nucleoside diphosphate derivatives, and several enzymatic activities were tentatively identified. In addition to representatives of known Nudix hydrolase subfamilies active on ADP-ribose, NADH, dinucleoside polyphosphates or (deoxy)nucleoside triphosphates, two new enzymes, a UDP-glucose pyrophosphatase and a CoA pyrophosphatase, were identified.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Xu W,Shen J,Dunn CA,Desai S,Bessman MJdoi
10.1046/j.1365-2958.2001.02267.xsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
286-90issue
2eissn
0950-382Xissn
1365-2958pii
mmi2267journal_volume
39pub_type
杂志文章abstract::Slipped-strand mispairing at sites containing so-called coding repeats (CRs) can lead to phase variation of surface proteins in Gram-negative bacteria. This mechanism, believed to contribute to virulence, has so far not been identified in a Gram-positive bacterium. In the genome of the Gram-positive human pathogen Str...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02493.x
更新日期:2001-06-01 00:00:00
abstract::The heptacistronic dnaK heat shock operon of Bacillus subtilis consists of the genes hrcA, grpE, dnaK, dnaJ, orf35, orf28 and orf50. It is controlled by the CIRCE/HrcA operator/repressor system and specifies three primary transcripts, two of which are processed into three different products. We have analysed the regul...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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abstract::Aspergillus nidulans was used as a model organism to investigate the fungal propionate metabolism and the mechanism of growth inhibition by propionate. The fungus is able to grow slowly on propionate as sole carbon and energy source. Propionate is oxidized to pyruvate via the methylcitrate cycle. The key enzyme methyl...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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更新日期:2002-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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更新日期:2003-11-01 00:00:00
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更新日期:2004-07-01 00:00:00
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更新日期:2010-12-01 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00