The endosomal sorting complex required for transport machinery influences haem uptake and capsule elaboration in Cryptococcus neoformans.

abstract：:Molecular analyses were applied to the genes encoding variants of the serosubtyping antigen, the class 1 outer membrane protein (PorA), from 55 serogroup A Neisseria meningitidis strains. These genes were evolutionarily stable and exhibited a limited range of genetic variation, primarily generated by recombination. Tr...

journal_title：Molecular microbiology

authors： Suker J,Feavers IM,Achtman M,Morelli G,Wang JF,Maiden MC

更新日期：1994-04-01 00:00:00

abstract：:Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cel...

journal_title：Molecular microbiology

authors： Ebersbach G,Ringgaard S,Møller-Jensen J,Wang Q,Sherratt DJ,Gerdes K

更新日期：2006-09-01 00:00:00

abstract：:We previously observed that hypoxia is an important component of host microenvironments during pulmonary fungal infections. However, mechanisms of fungal growth in these in vivo hypoxic conditions are poorly understood. Here, we report that mitochondrial respiration is active in hypoxia (1% oxygen) and critical for fu...

journal_title：Molecular microbiology

authors： Grahl N,Dinamarco TM,Willger SD,Goldman GH,Cramer RA

更新日期：2012-04-01 00:00:00

abstract：:The lipopolysaccharide (LPS) of H. influenzae is highly variable. Much of the structural diversity is derived from phase variation, or high frequency on-off switching, of molecules attached during LPS biosynthesis. In this study, we examined the dynamics of LPS phase variation following exposure to human serum as a so...

journal_title：Molecular microbiology

authors： Clark SE,Eichelberger KR,Weiser JN

更新日期：2013-05-01 00:00:00

abstract：:The fibronectin binding protein, FnBPA, is a multifunctional microbial surface component recognizing adhesive matrix molecule (MSCRAMM) that promotes bacterial adherence to immobilized fibrinogen and elastin via the N-terminal A domain. The binding site for fibrinogen and elastin was localized to subdomains N2N3. A th...

journal_title：Molecular microbiology

authors： Keane FM,Loughman A,Valtulina V,Brennan M,Speziale P,Foster TJ

更新日期：2007-02-01 00:00:00

abstract：:Bacterial cytokinesis is driven by the septal ring apparatus, the assembly of which in Escherichia coli is directed to mid-cell by the Min system. Despite suffering aberrant divisions at the poles, cells lacking the minCDE operon (Min(-)) have an almost normal growth rate. We developed a generally applicable screening...

journal_title：Molecular microbiology

authors： Bernhardt TG,de Boer PA

更新日期：2004-06-01 00:00:00

abstract：:The increase in bacterial resistance to multiple drugs represents a serious and growing health risk. One component of multidrug resistance (MDR) is a group of multidrug transporters that are often regulated at the transcriptional level by repressors and/or activators. Some of these transcription factors are also multi...

journal_title：Molecular microbiology

authors： Schumacher MA,Brennan RG

更新日期：2002-08-01 00:00:00

abstract：:Era is a low-molecular-weight GTPase essential for Escherichia coli viability. The gene encoding Era is found in the rnc operon, and the synthesis of both RNase III and Era increases with growth rate. Mutants that are partially defective in Era GTPase activity or that are reduced in the synthesis of wild-type Era beco...

journal_title：Molecular microbiology

authors： Britton RA,Powell BS,Dasgupta S,Sun Q,Margolin W,Lupski JR,Court DL

更新日期：1998-02-01 00:00:00

abstract：:Previous evidence showed that b- and a-type flagellins of Pseudomonas aeruginosa are modified in vivo by phosphorylation at tyrosine. This research was designed to demonstrate phosphorylation of flagellin at tyrosine in vitro. Evidence presented showed that flagellin is labelled by [gamma-32P]-ATP, but not by [alpha-3...

journal_title：Molecular microbiology

authors： South SL,Nichols R,Montie TC

更新日期：1994-06-01 00:00:00

abstract：:The non-essential RGD1 gene from Saccharomyces cerevisiae encodes a protein that has been characterized in vitro as a Rho GTPase activating protein (RhoGAP) for the Rho3 and Rho4 proteins. Rgd1p, which displays a conserved FCH-coiled coil-Rho-GAP domain organization, showed a patch-like distribution in the cell, inclu...

journal_title：Molecular microbiology

authors： Roumanie O,Peypouquet MF,Bonneu M,Thoraval D,Doignon F,Crouzet M

更新日期：2000-06-01 00:00:00

abstract：:We report here the construction and analysis of insertional mutations in each of the three genes of the gltBDF operon and the nucleotide sequence of the region downstream from gltD. Two open reading frames were identified, the first of which corresponds to gltF. The gltB and gltD genes code for the large and small sub...

journal_title：Molecular microbiology

authors： Castaño I,Flores N,Valle F,Covarrubias AA,Bolivar F

更新日期：1992-09-01 00:00:00

abstract：:The de novo purine biosynthetic enzymes 5-amino-4-imidazolecarboxamide-ribonucleotide (AICAR) transformylase (EC 2.1.2.3), IMP cyclohydrolase (EC 3.5.4.10) and glycineamide-ribonucleotide (GAR) synthetase (EC 2.1.2.2) are encoded by the purHD locus of Escherichia coli. The DNA sequence of this locus revealed two open ...

journal_title：Molecular microbiology

authors： Flannigan KA,Hennigan SH,Vogelbacker HH,Gots JS,Smith JM

更新日期：1990-03-01 00:00:00

abstract：:Peptide transport in microorganisms is important for nutrition of the cell and various signalling processes including regulation of gene expression, sporulation, chemotaxis, competence and virulence development. Peptide transport is mediated via different combinations of ion-linked and ATP-binding cassette (ABC) trans...

journal_title：Molecular microbiology

authors： Doeven MK,Kok J,Poolman B

更新日期：2005-08-01 00:00:00

abstract：:Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...

journal_title：Molecular microbiology

authors： Pagliarulo C,Salvatore P,De Vitis LR,Colicchio R,Monaco C,Tredici M,Talà A,Bardaro M,Lavitola A,Bruni CB,Alifano P

更新日期：2004-03-01 00:00:00

abstract：:The pathogenicity of Plasmodium falciparum is partly due to parasite-induced host cell modifications. These modifications are facilitated by exported P. falciparum proteins, collectively referred to as the exportome. Export of several hundred proteins is mediated by the PEXEL/HT, a protease cleavage site. The PEXEL/HT...

journal_title：Molecular microbiology

authors： Schulze J,Kwiatkowski M,Borner J,Schlüter H,Bruchhaus I,Burmester T,Spielmann T,Pick C

更新日期：2015-07-01 00:00:00

abstract：:Myxococcus xanthus is a Gram-negative bacterium capable of complex developmental processes involving vegetative swarming and fruiting body formation. Social (S-) gliding motility, one of the two motility systems used by M. xanthus, requires at least two cell surface structures: type IV pili (TFP) and extracellular pol...

journal_title：Molecular microbiology

authors： Yang Z,Lux R,Hu W,Hu C,Shi W

更新日期：2010-06-01 00:00:00

abstract：:The genome of the malaria parasite Plasmodium falciparum contains several multicopy gene families, including var, rifin, stevor and Pfmc-2TM. These gene families undergo expression switching and appear to play a role in antigenic variation. It has recently been shown that forcing parasites to express high copy numbers...

journal_title：Molecular microbiology

authors： Howitt CA,Wilinski D,Llinás M,Templeton TJ,Dzikowski R,Deitsch KW

更新日期：2009-09-01 00:00:00

abstract：:Iron is a critical nutrient for the growth and survival of most bacterial species. Accordingly, much attention has been paid to the mechanisms by which host organisms sequester iron from invading bacteria and how bacteria acquire iron from their environment. However, under oxidative stress conditions such as those enc...

journal_title：Molecular microbiology

authors： Frawley ER,Fang FC

更新日期：2014-08-01 00:00:00

abstract：:The 136 codon (408 bp) denA gene encoding endonuclease II (Endoll) of bacteriophage T4 was unambiguously identified through sequencing and subsequent cloning. Endoll prepared from cloned DNA through coupled in vitro transcription-translation nicked and cut DNA in vitro in a sequence-specific manner. In vitro (and in v...

journal_title：Molecular microbiology

authors： Carlson K,Kosturko LD,Nyström AC

更新日期：1999-03-01 00:00:00

abstract：:Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease. Here, we show that another critical cell cycle regulator, SciP, is also degraded...

journal_title：Molecular microbiology

authors： Gora KG,Cantin A,Wohlever M,Joshi KK,Perchuk BS,Chien P,Laub MT

更新日期：2013-03-01 00:00:00

abstract：:Klebsiella pneumoniae strain PAP996 was previously shown to secrete fatty acylated, aggregated (micellar) pullulanase only after the end of exponential growth. Here we show that the closely related strain K21 secretes large amounts of unacylated, non-aggregated (monomeric) pullulanase during exponential growth. Only a...

journal_title：Molecular microbiology

authors： Kornacker MG,Boyd A,Pugsley AP,Plastow GS

更新日期：1989-04-01 00:00:00

abstract：:Only few small, regulatory RNAs encoded opposite another gene have been identified in bacteria. Here, we report the characterization of a locus where a small RNA (SymR) is encoded in cis to an SOS-induced gene whose product shows homology to the antitoxin MazE (SymE). Synthesis of the SymE protein is tightly repressed...

journal_title：Molecular microbiology

authors： Kawano M,Aravind L,Storz G

更新日期：2007-05-01 00:00:00

abstract：:Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here,...

journal_title：Molecular microbiology

authors： Davis R,Écija-Conesa A,Gallego-Jara J,de Diego T,Filippova EV,Kuffel G,Anderson WF,Gibson BW,Schilling B,Canovas M,Wolfe AJ

更新日期：2018-01-01 00:00:00

abstract：:In this paper we show that the Escherichia coli protein Fis has a regulatory function in Mu transposition in the presence of Mu repressor. Fis can lower the transposition frequency of a mini-Mu 3-80-fold, but only if the Mu repressor is expressed simultaneously. In this novel type of regulation of transposition by the...

journal_title：Molecular microbiology

authors： van Drunen CM,van Zuylen C,Mientjes EJ,Goosen N,van de Putte P

更新日期：1993-10-01 00:00:00

abstract：:N-terminal protein acetylation is common in eukaryotes and halophilic archaea, but very rare in bacteria. We demonstrate that some of the most abundant proteins present in the crenarchaeote Sulfolobus solfataricus, including subunits of the thermosome, proteosome and ribosome, are acetylated at the N-terminus. Modific...

journal_title：Molecular microbiology

authors： Mackay DT,Botting CH,Taylor GL,White MF

更新日期：2007-06-01 00:00:00

abstract：:The cyclic AMP protein kinase A pathway governs numerous biological features of the fungal pathogen Candida albicans. The catalytic protein kinase A subunits, Tpk1 (orf19.4892) and Tpk2 (orf19.2277), have divergent roles, and most studies indicate a more pronounced role for Tpk2. Here we dissect two Tpk1-responsive pr...

journal_title：Molecular microbiology

authors： Fanning S,Xu W,Beaurepaire C,Suhan JP,Nantel A,Mitchell AP

更新日期：2012-10-01 00:00:00

abstract：:We previously identified three well-dispersed mutations, E978-K, F989-L and D1009-R within the haemolysin A signal region, located at positions -46, -35 and -15, with respect to the C-terminus, respectively. Each mutation reduces the efficiency of secretion two- to threefold leaving 30-45% of the wild-type activity. W...

journal_title：Molecular microbiology

authors： Kenny B,Chervaux C,Holland IB

更新日期：1994-01-01 00:00:00

abstract：:Only one species of Shigella, Shigella dysenteriae 1, has been demonstrated to produce Shiga toxin (Stx). Stx is closely related to the toxins produced by Shiga toxin-producing Escherichia coli (STEC). In STEC, these toxins are often encoded on lambdoid bacteriophages and are major virulence factors for these organism...

journal_title：Molecular microbiology

authors： McDonough MA,Butterton JR

更新日期：1999-12-01 00:00:00

abstract：:A two-hybrid approach was applied to screen for proteins with the ability to interact with PHB synthase (PhaC1) of Ralstonia eutropha. The H16_A0141 gene (phaM) was identified in the majority of positive clones. PhaM (26.6 kDa) strongly interacted with PhaC1 and with phasin PhaP5 but not with PhaP1 or other PHB granul...

journal_title：Molecular microbiology

authors： Pfeiffer D,Wahl A,Jendrossek D

更新日期：2011-11-01 00:00:00

abstract：:The O-antigen of Salmonella lipopolysaccharide is a major antigenic determinant and its chemical composition forms the basis for Salmonella serotyping. Modifications of the O-antigen that can affect the serotype include those carried out by the products of glycosyltransferase operons (gtr), which are present on specif...

journal_title：Molecular microbiology

authors： Broadbent SE,Davies MR,van der Woude MW

更新日期：2010-07-01 00:00:00