Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro.

abstract：:We have cloned the rpoD gene encoding the principal sigma (sigma) factor of Helicobacter pylori. The deduced amino acid sequence reveals a predicted polypeptide of 676 residues that has amino acid homology with the principal sigma factors of a number of divergent prokaryotes. We have designated this factor sigma80. Am...

journal_title：Molecular microbiology

authors： Beier D,Spohn G,Rappuoli R,Scarlato V

更新日期：1998-10-01 00:00:00

abstract：:Pathogenic enterotoxigenic Escherichia coli (ETEC) are the major bacterial cause of diarrhea in young children in developing countries and in travelers, causing significant mortality in children. Adhesive fimbriae are a prime virulence factor for ETEC, initiating colonization of the small intestinal epithelium. Simila...

journal_title：Molecular microbiology

authors： Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

更新日期：2015-01-01 00:00:00

abstract：:The group A streptococcus (GAS) causes a variety of human diseases, including toxic shock syndrome and necrotizing fasciitis, which are both associated with significant mortality. Even the superficial self-limiting diseases caused by GAS, such as pharyngitis, impose a significant economic burden on society. GAS can ca...

journal_title：Molecular microbiology

authors： Churchward G

更新日期：2007-04-01 00:00:00

abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...

journal_title：Molecular microbiology

authors： Delany I,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-12-01 00:00:00

abstract：:Osmoregulatory mutants of Saccharomyces cerevisiae with a defect in their capacity to readjust the cell volume/buoyant density after osmotically induced dehydration were enriched by density gradient centrifugation. Colonies derived from cells that remained dense after dehydration were screened for sensitivity to high ...

journal_title：Molecular microbiology

authors： Larsson K,Ansell R,Eriksson P,Adler L

更新日期：1993-12-01 00:00:00

abstract：:Synthesis and secretion of the 110kDa haemolysin toxin of Escherichia coli and other pathogenic Gram-negative bacteria are governed by the four genes of the hly operon. We have identified, by transposon mutagenesis, an E. coli cellular locus, hlyT, required for the synthesis and secretion of haemolysin encoded in tran...

journal_title：Molecular microbiology

authors： Bailey MJ,Koronakis V,Schmoll T,Hughes C

更新日期：1992-04-01 00:00:00

abstract：:The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

journal_title：Molecular microbiology

authors： Outten FW,Djaman O,Storz G

更新日期：2004-05-01 00:00:00

abstract：:The fluidity and phase state of bacterial lipid bilayers commonly change in response to ambient environmental conditions to maintain the critical functions of the envelope as a semipermeable and selective boundary. A special, and intricate, set of alterations in membrane lipid metabolism is elicited by conditions caus...

journal_title：Molecular microbiology

authors： DiRusso CC,Nyström T

更新日期：1998-01-01 00:00:00

abstract：:Transposon mutagenesis was used to isolate insertion mutants of the photosynthetic bacterium Rhodobacter capsulatus which were unable to grow under aerobic conditions in the dark on malate, succinate or fumarate as sole carbon sources. Of five mutants isolated, all were deficient in C4-dicarboxylate transport. However...

journal_title：Molecular microbiology

authors： Hamblin MJ,Shaw JG,Curson JP,Kelly DJ

更新日期：1990-09-01 00:00:00

abstract：:Strains of Rhizobium leguminosarum biovar viciae specifically make an abundant protein (Rhi) in free-living culture but not in bacteroids. Genes needed for Rhi synthesis are on a Sym plasmid and here we show that one of these genes, rhiA, is the structural gene that specifies this polypeptide. Transcription of rhiA re...

journal_title：Molecular microbiology

authors： Economou A,Hawkins FK,Downie JA,Johnston AW

更新日期：1989-01-01 00:00:00

abstract：:Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysi...

journal_title：Molecular microbiology

authors： Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A

更新日期：2004-10-01 00:00:00

abstract：:The phenomenon of cell-density-dependent control of gene expression, called autoinduction, has long been a subject of interest and investigation in bioluminescent marine bacteria. It is now becoming clear that many other bacteria, including animal and plant pathogens, use an autoinduction mechanism to regulate a varie...

journal_title：Molecular microbiology

authors： Sitnikov DM,Schineller JB,Baldwin TO

更新日期：1995-09-01 00:00:00

abstract：:In Candida glabrata, the sirtuins Sir2 and Hst1 control the expression of a wide number of genes including adhesins required for host colonization and niacin transporters needed for growth. Given that these sirtuins can be inactivated during infection, we asked if their inhibition could modify the response of C. glabr...

journal_title：Molecular microbiology

authors： Orta-Zavalza E,Guerrero-Serrano G,Gutiérrez-Escobedo G,Cañas-Villamar I,Juárez-Cepeda J,Castaño I,De Las Peñas A

更新日期：2013-06-01 00:00:00

abstract：:MsDef1 and MtDef4 from Medicago spp. are small cysteine-rich defensins with potent antifungal activity against a broad range of filamentous fungi. Each defensin has a hallmark γ-core motif (GXCX(3-9) C), which contains major determinants of its antifungal activity. In this study, the antifungal activities of MsDef1, M...

journal_title：Molecular microbiology

authors： Muñoz A,Chu M,Marris PI,Sagaram US,Kaur J,Shah DM,Read ND

更新日期：2014-06-01 00:00:00

abstract：:The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

journal_title：Molecular microbiology

authors： O Cróinín T,Dorman CJ

更新日期：2007-10-01 00:00:00

abstract：:Inhibitors of protein synthesis cause defects in the assembly of ribosomal subunits. In response to treatment with the antibiotics erythromycin or chloramphenicol, precursors of both large and small ribosomal subunits accumulate. We have used a pulse-labelling approach to demonstrate that the accumulating subribosomal...

journal_title：Molecular microbiology

authors： Siibak T,Peil L,Dönhöfer A,Tats A,Remm M,Wilson DN,Tenson T,Remme J

更新日期：2011-04-01 00:00:00

abstract：:Staphylococcus aureus strain M produces large amounts of capsular polysaccharide. It produces a non-encapsulated variant at a frequency of 0.01% at 37 degrees C. At high temperature (43 degrees C), the frequency of capsule loss was shown to be 1-38%. A 19 kb plasmid and a prophage were found to be carried by the M str...

journal_title：Molecular microbiology

authors： Lee CY

更新日期：1992-06-01 00:00:00

abstract：:Escherichia coli cells use two distinct sensory circuits during chemotaxis towards carbohydrates. One circuit requires the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and is independent of any specific chemoreceptor, whereas the other uses a chemoreceptor-dependent sensory mechanism analogous to that...

journal_title：Molecular microbiology

authors： Kristich CJ,Glekas GD,Ordal GW

更新日期：2003-03-01 00:00:00

abstract：:To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expre...

journal_title：Molecular microbiology

authors： Wells JM,Wilson PW,Norton PM,Gasson MJ,Le Page RW

更新日期：1993-06-01 00:00:00

abstract：:The genetic organization of the spoVAF-serA area of the Bacillus subtilis chromosome and its putative transcription map have been derived from analysis of the nucleotide sequence. In order to confirm this transcription map as regards size of transcripts and to determine growth conditions for their appearance, we under...

journal_title：Molecular microbiology

authors： Azevedo V,Sorokin A,Ehrlich SD,Serror P

更新日期：1993-10-01 00:00:00

abstract：:The stringent response is important for bacterial survival under stressful conditions, such as amino acid starvation, and is characterized by the accumulation of ppGpp and pppGpp. ObgE (CgtA, YhbZ) is an essential conserved GTPase in Escherichia coli and several observations have implicated the protein in the control ...

journal_title：Molecular microbiology

authors： Persky NS,Ferullo DJ,Cooper DL,Moore HR,Lovett ST

更新日期：2009-07-01 00:00:00

abstract：:Sequence-inherent targeting information directs polypeptides synthesized in the cytosol to their respective cellular compartment. Some proteins use ambiguous sorting signals or specific folding properties to be dually distributed between the cytosol and mitochondria. A study published in this issue of Molecular Microb...

journal_title：Molecular microbiology

authors： Herrmann JM

更新日期：2009-04-01 00:00:00

abstract：:Cell division in most bacteria is directed by FtsZ, a conserved tubulin-like GTPase that assembles forming the cytokinetic Z-ring and constitutes a target for the discovery of new antibiotics. The developmental regulator MciZ, a 40-amino acid peptide endogenously produced during Bacillus subtilis sporulation, halts cy...

journal_title：Molecular microbiology

authors： Araújo-Bazán L,Huecas S,Valle J,Andreu D,Andreu JM

更新日期：2019-04-01 00:00:00

abstract：:Rpc25 is a strongly conserved subunit of RNA polymerase III with homology to Rpa43 in RNA polymerase I, Rpb7 in RNA polymerase II and the archaeal RpoE subunit. A central domain of Rpc25 can replaced the corresponding region of Rpb7 with little or no growth defect, underscoring the functional relatedness of these prot...

journal_title：Molecular microbiology

authors： Zaros C,Thuriaux P

更新日期：2005-01-01 00:00:00

abstract：:Escherichia coli RNA polymerase is able to site-specifically melt 12 bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA polymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymera...

journal_title：Molecular microbiology

authors： deHaseth PL,Helmann JD

更新日期：1995-06-01 00:00:00

abstract：:RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region. In principle, hp2 could function either as a cleavage site for RNase E or as a binding si...

journal_title：Molecular microbiology

authors： Schuck A,Diwa A,Belasco JG

更新日期：2009-04-01 00:00:00

abstract：:A major obstacle to tuberculosis (TB) control is the problem of chronic TB infection (CTBI). Here we report that 5'-adenosinephosphosulphate reductase (CysH), an enzyme essential for the production of reduced-sulphur-containing metabolites, is critical for Mycobacterium tuberculosis (Mtb) survival in chronic infection...

journal_title：Molecular microbiology

authors： Senaratne RH,De Silva AD,Williams SJ,Mougous JD,Reader JR,Zhang T,Chan S,Sidders B,Lee DH,Chan J,Bertozzi CR,Riley LW

更新日期：2006-03-01 00:00:00

abstract：:Differential expression of the genes expressing Pap pili in Escherichia coli was suggested to involve mRNAs with different stabilities. As the result of a post-transcriptional processing event, a papA gene-specific mRNA product (mRNA-A) accumulates in large excess relative to the primary mRNA-BA transcript. Our result...

journal_title：Molecular microbiology

authors： Nilsson P,Uhlin BE

更新日期：1991-07-01 00:00:00

abstract：:Nitrogen-regulated genes in enterobacteria are positively controlled by the transcriptional activator of σ(N) -dependent promoters NtrC, either directly or indirectly, through the dual regulator Nac. Similar to enterobacteria, gdhA encoding glutamate dehydrogenase from Pseudomonas putida is one of the few genes that i...

journal_title：Molecular microbiology

authors： Hervás AB,Canosa I,Santero E

更新日期：2010-10-01 00:00:00

abstract：:A series of lipoproteins was detected in the membrane fraction of Yersinia enterocolitica W227, a typical strain from serotype O:9. At least two of them, YlpA and YlpB, are encoded by the pYV plasmid. The sequence of ylpA reveals the presence of a typical lipoprotein signal peptide. The mature YlpA protein would be 22...

journal_title：Molecular microbiology

authors： China B,Michiels T,Cornelis GR

更新日期：1990-09-01 00:00:00