Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel.

Abstract:

:Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysis of the entire collection of the resistance peptides allowed their classification into five distinct groups according to their sequence similarity and the type of resistance they confer. A strong correlation was observed between the structures of macrolide antibiotics and sequences of the peptides conferring resistance. Such a correlation indicates that sequence-specific interactions between the nascent peptide and the macrolide antibiotic and/or the ribosome can occur in the ribosomal exit tunnel.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A

doi

10.1111/j.1365-2958.2004.04290.x

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

376-85

issue

2

eissn

0950-382X

issn

1365-2958

pii

MMI4290

journal_volume

54

pub_type

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