Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel.


:Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysis of the entire collection of the resistance peptides allowed their classification into five distinct groups according to their sequence similarity and the type of resistance they confer. A strong correlation was observed between the structures of macrolide antibiotics and sequences of the peptides conferring resistance. Such a correlation indicates that sequence-specific interactions between the nascent peptide and the macrolide antibiotic and/or the ribosome can occur in the ribosomal exit tunnel.


Mol Microbiol


Molecular microbiology


Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A




Has Abstract


2004-10-01 00:00:00














  • Involvement of the amino-terminal phosphorylation module of UhpA in activation of uhpT transcription in Escherichia coli.

    abstract::The UhpA protein is required for expression of the sugar phosphate transporter UhpT in Escherichia coli and is regulated by phosphate transfer from the transmembrane UhpBC sensor kinase complex. UhpA action requires the sensor kinase complex and the site of phosphorylation, Asp-54, under normal conditions, but not whe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Webber CA,Kadner RJ

    更新日期:1997-06-01 00:00:00

  • Live cell imaging of SOS and prophage dynamics in isogenic bacterial populations.

    abstract::Almost all bacterial genomes contain DNA of viral origin, including functional prophages or degenerated phage elements. A frequent but often unnoted phenomenon is the spontaneous induction of prophage elements (SPI) even in the absence of an external stimulus. In this study, we have analyzed SPI of the large, degenera...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Helfrich S,Pfeifer E,Krämer C,Sachs CC,Wiechert W,Kohlheyer D,Nöh K,Frunzke J

    更新日期:2015-11-01 00:00:00

  • Requirement of essential Pbp2x and GpsB for septal ring closure in Streptococcus pneumoniae D39.

    abstract::Bacterial cell shapes are manifestations of programs carried out by multi-protein machines that synthesize and remodel the resilient peptidoglycan (PG) mesh and other polymers surrounding cells. GpsB protein is conserved in low-GC Gram-positive bacteria and is not essential in rod-shaped Bacillus subtilis, where it pl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Land AD,Tsui HC,Kocaoglu O,Vella SA,Shaw SL,Keen SK,Sham LT,Carlson EE,Winkler ME

    更新日期:2013-12-01 00:00:00

  • Light-dependent gene activation in Aspergillus nidulans is strictly dependent on phytochrome and involves the interplay of phytochrome and white collar-regulated histone H3 acetylation.

    abstract::The ability for light sensing is found from bacteria to humans but relies only on a small number of evolutionarily conserved photoreceptors. A large number of fungi react to light, mostly to blue light. Aspergillus nidulans also responds to red light using a phytochrome light sensor, FphA, for the control of hundreds ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hedtke M,Rauscher S,Röhrig J,Rodríguez-Romero J,Yu Z,Fischer R

    更新日期:2015-08-01 00:00:00

  • Lactococcus lactis: high-level expression of tetanus toxin fragment C and protection against lethal challenge.

    abstract::To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expre...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wells JM,Wilson PW,Norton PM,Gasson MJ,Le Page RW

    更新日期:1993-06-01 00:00:00

  • Proteomic and functional analysis of the suite of Ysp proteins exported by the Ysa type III secretion system of Yersinia enterocolitica Biovar 1B.

    abstract::Full virulence of Yersinia enterocolitica Biovar 1B requires two distinct and distantly related contact-dependent type III secretion (T3S) systems. The plasmid-encoded Ysc T3S system is essential for systemic stages of infection and the Yop effector proteins it translocates have been extensively studied. The chromosom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Matsumoto H,Young GM

    更新日期:2006-01-01 00:00:00

  • Regulated proteolysis of a transcription factor complex is critical to cell cycle progression in Caulobacter crescentus.

    abstract::Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease. Here, we show that another critical cell cycle regulator, SciP, is also degraded...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gora KG,Cantin A,Wohlever M,Joshi KK,Perchuk BS,Chien P,Laub MT

    更新日期:2013-03-01 00:00:00

  • Protein exporter function and in vitro ATPase activity are correlated in ABC-domain mutants of HlyB.

    abstract::The Escherichia coli toxin exporter HlyB comprises an integral membrane domain fused to a cytoplasmic domain of the ATP-binding cassette (ABC) super-family, and it directs translocation of the 110kDa haemolysin protein out of the bacterial cell without using an N-terminal secretion signal peptide. We have exploited th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Koronakis E,Hughes C,Milisav I,Koronakis V

    更新日期:1995-04-01 00:00:00

  • gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression.

    abstract::We report here the construction and analysis of insertional mutations in each of the three genes of the gltBDF operon and the nucleotide sequence of the region downstream from gltD. Two open reading frames were identified, the first of which corresponds to gltF. The gltB and gltD genes code for the large and small sub...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Castaño I,Flores N,Valle F,Covarrubias AA,Bolivar F

    更新日期:1992-09-01 00:00:00

  • Hexose phosphorylation and the putative calcium channel component Mid1p are required for the hexose-induced transient elevation of cytosolic calcium response in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae responds to environ-mental stimuli such as an exposure to pheromone or to hexoses after carbon source limitation with a transient elevation of cytosolic calcium (TECC) response. In this study, we examined whether hexose transport and phosphorylation are necessary for the TECC response. We foun...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tökés-Füzesi M,Bedwell DM,Repa I,Sipos K,Sümegi B,Rab A,Miseta A

    更新日期:2002-06-01 00:00:00

  • Carbon catabolite repression of the Aspergillus nidulans xlnA gene.

    abstract::Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Orejas M,MacCabe AP,Pérez González JA,Kumar S,Ramón D

    更新日期:1999-01-01 00:00:00

  • Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter.

    abstract::Bacterial autotransporters consist of an N-terminal 'passenger domain' that is transported into the extracellular space by an unknown mechanism and a C-terminal 'beta-domain' that forms a beta-barrel in the outer membrane. Recent studies have revealed that fully assembled autotransporters have an unusual architecture ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ieva R,Skillman KM,Bernstein HD

    更新日期:2008-01-01 00:00:00

  • Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes.

    abstract::bfp, the structural gene of the major repeating bundle-forming pilus (BFP) subunit, was cloned from the enteroadherent factor (EAF) plasmid of enteropathogenic Escherichia coli (EPEC) strain B171 (O111:NM). The bfp open reading frame encoded a 193-amino-acid protein; comparison of this sequence with the biochemically ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sohel I,Puente JL,Murray WJ,Vuopio-Varkila J,Schoolnik GK

    更新日期:1993-02-01 00:00:00

  • Heterologous expression and self-assembly of the S-layer protein SbsA of Bacillus stearothermophilus in Escherichia coli.

    abstract::The cell surface of Bacillus stearothermophilus PV72 is covered by a regular surface layer (S-layer) composed of single species of protein, SbsA, with a molecular weight of 130,000. Recently, the sequence of the corresponding gene (sbsA) has been determined. The SbsA coding region including the signal sequence was clo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kuen B,Sára M,Lubitz W

    更新日期:1996-02-01 00:00:00

  • Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes.

    abstract::Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ding Q,Kusano S,Villarejo M,Ishihama A

    更新日期:1995-05-01 00:00:00

  • BrgE is a regulator of Myxococcus xanthus development.

    abstract::We report here the identification and characterization of a member of the Myxococcus xanthus SdeK signal transduction pathway, BrgE. This protein was identified as an SdeK-interacting component using a yeast two-hybrid screen, and we further confirmed this interaction by the glutathione S-transferase (GST) pulldown as...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pham VD,Shebelut CW,Zumstein EJ,Singer M

    更新日期:2005-08-01 00:00:00

  • The transferrin-iron import system from pathogenic Neisseria species.

    abstract::Two pathogenic species within the genus Neisseria cause the diseases gonorrhoea and meningitis. While vaccines are available to protect against four N. meningitidis serogroups, there is currently no commercial vaccine to protect against serogroup B or against N. gonorrhoeae. Moreover, the available vaccines have signi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Noinaj N,Buchanan SK,Cornelissen CN

    更新日期:2012-10-01 00:00:00

  • Site-directed mutagenesis of the katG gene of Mycobacterium tuberculosis: effects on catalase-peroxidase activities and isoniazid resistance.

    abstract::Recent studies examining the molecular mechanisms of isoniazid (INH) resistance in Mycobacterium tuberculosis have demonstrated that a significant percentage of drug-resistant strains are mutated in the katG gene which encodes a catalase-peroxidase, and the majority of these alterations are missense mutations which re...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rouse DA,DeVito JA,Li Z,Byer H,Morris SL

    更新日期:1996-11-01 00:00:00

  • A yeast genetic system for the identification and characterization of substrate proteins transferred into host cells by the Legionella pneumophila Dot/Icm system.

    abstract::The Dot/Icm system is a type IVb secretion system used by Legionella pneumophila to modulate vesicular transport in both protozoan and mammalian host cells. It has been shown that proteins and processes that are highly conserved in all eukaryotic cells are targets for some of the proteins injected by the Dot/Icm syste...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Campodonico EM,Chesnel L,Roy CR

    更新日期:2005-05-01 00:00:00

  • Assembly and interactions of cotJ-encoded proteins, constituents of the inner layers of the Bacillus subtilis spore coat.

    abstract::During Bacillus subtilis endospore formation, a complex protein coat is assembled around the maturing spore. The coat is made up of more than two dozen proteins that form an outer layer, which provides chemical resistance, and an inner layer, which may play a role in the activation of germination. A third, amorphous l...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Seyler RW Jr,Henriques AO,Ozin AJ,Moran CP Jr

    更新日期:1997-09-01 00:00:00

  • Rga5p is a specific Rho1p GTPase-activating protein that regulates cell integrity in Schizosaccharomyces pombe.

    abstract::Schizosaccharomyces pombe Rho1p regulates (1,3)beta-d-glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different S. pombe genes code for proteins predicted to act as Rho GTPase-activating protei...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Calonge TM,Arellano M,Coll PM,Perez P

    更新日期:2003-01-01 00:00:00

  • Evidence that F-plasmid proteins TraV, TraK and TraB assemble into an envelope-spanning structure in Escherichia coli.

    abstract::We have examined the role of the F-plasmid TraV outer membrane lipoprotein in the assembly of F-pili. Yeast two-hybrid analysis with a traV bait repeatedly identified traK, which is predicted to encode a periplasmic protein, among positive prey plasmids. A traK bait in turn identified traV and traB, which is predicted...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Harris RL,Hombs V,Silverman PM

    更新日期:2001-11-01 00:00:00

  • RutR is the uracil/thymine-sensing master regulator of a set of genes for synthesis and degradation of pyrimidines.

    abstract::Using the genomic SELEX, a total of six Escherichia coli DNA fragments have been identified, which formed complexes with transcription factor RutR. The RutR regulon was found to include a large number of genes encoding components for not only degradation of pyrimidines but also transport of glutamate, synthesis of glu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Shimada T,Hirao K,Kori A,Yamamoto K,Ishihama A

    更新日期:2007-11-01 00:00:00

  • The FNR-type transcriptional regulator SinR controls maturation of Agrobacterium tumefaciens biofilms.

    abstract::Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutan...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ramey BE,Matthysse AG,Fuqua C

    更新日期:2004-06-01 00:00:00

  • In vitro characterization of constitutive CysB proteins from Salmonella typhimurium.

    abstract::Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Colyer TE,Kredich NM

    更新日期:1996-07-01 00:00:00

  • Biochemical analysis and structure determination of Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 muteins reveal the PHB binding site and details of substrate-enzyme interactions.

    abstract::Five amino acids (Y105, Y176, Y189, Y189, W207) that constitute the substrate binding site of PHB depolymerase PhaZ7 were identified. All residues are located at a single surface-exposed location of PhaZ7. Exchange of these amino acids by less hydrophobic, hydrophilic or negatively charged residues reduced binding of ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Jendrossek D,Hermawan S,Subedi B,Papageorgiou AC

    更新日期:2013-11-01 00:00:00

  • Light and oxygen effects share a common regulatory DNA sequence in Rhodobacter capsulatus.

    abstract::External factors regulate the formation of pigment protein complexes in facultatively photosynthetic bacteria. The puf operon of Rhodobacter capsulatus encodes the pigment binding proteins of the reaction centre and light-harvesting I complex. Here we demonstrate that a single base-pair exchange within a sequence of d...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Klug G,Gad'on N,Jock S,Narro ML

    更新日期:1991-05-01 00:00:00

  • A role for the FtsQLB complex in cytokinetic ring activation revealed by an ftsL allele that accelerates division.

    abstract::The cytokinetic apparatus of bacteria is initially formed by the polymerization of the tubulin-like FtsZ protein into a ring structure at midcell. This so-called Z-ring facilitates the recruitment of many additional proteins to the division site to form the mature divisome machine. Although the assembly pathway leadin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tsang MJ,Bernhardt TG

    更新日期:2015-03-01 00:00:00

  • Pre-replication assembly of E. coli replisome components.

    abstract::The localization of SeqA, thymidylate synthase, DnaB (helicase) and the DNA polymerase components alpha and tau, has been studied by immunofluorescence microscopy. The origin has been labelled through GFP-LacI bound near oriC. SeqA was located in the cell centre for one replication factory (RF) and at 1/4 and 3/4 posi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: den Blaauwen T,Aarsman ME,Wheeler LJ,Nanninga N

    更新日期:2006-11-01 00:00:00

  • Hysteretic and graded responses in bacterial two-component signal transduction.

    abstract::Bacterial two-component systems (TCS) are key signal transduction networks regulating global responses to environmental change. Environmental signals may modulate the phosphorylation state of sensor kinases (SK). The phosphorylated SK transfers the phosphate to its cognate response regulator (RR), which causes physiol...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Igoshin OA,Alves R,Savageau MA

    更新日期:2008-06-01 00:00:00