Tandem DNA-bound cAMP-CRP complexes are required for transcriptional repression of the deoP2 promoter by the CytR repressor in Escherichia coli.

abstract：:The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence in the human fungal pathogen Candida albicans. It has long been thought that the PKA catalytic subunit is essential for cell viability in this fungus. Paradoxically, the single adenylyl c...

journal_title：Molecular microbiology

authors： Cao C,Wu M,Bing J,Tao L,Ding X,Liu X,Huang G

更新日期：2017-07-01 00:00:00

abstract：:Small GTP-binding proteins of the highly conserved Rho family act as molecular switches regulating cell signalling, cytoskeletal organization and vesicle trafficking in eukaryotic cells. Here we show that in the dimorphic plant pathogenic fungus Ustilago maydis deletion of either cdc42 or rac1 results in loss of virul...

journal_title：Molecular microbiology

authors： Mahlert M,Leveleki L,Hlubek A,Sandrock B,Bölker M

更新日期：2006-01-01 00:00:00

abstract：:Previous evidence showed that b- and a-type flagellins of Pseudomonas aeruginosa are modified in vivo by phosphorylation at tyrosine. This research was designed to demonstrate phosphorylation of flagellin at tyrosine in vitro. Evidence presented showed that flagellin is labelled by [gamma-32P]-ATP, but not by [alpha-3...

journal_title：Molecular microbiology

authors： South SL,Nichols R,Montie TC

更新日期：1994-06-01 00:00:00

abstract：:Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

journal_title：Molecular microbiology

authors： Ding Q,Kusano S,Villarejo M,Ishihama A

更新日期：1995-05-01 00:00:00

abstract：:RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region. In principle, hp2 could function either as a cleavage site for RNase E or as a binding si...

journal_title：Molecular microbiology

authors： Schuck A,Diwa A,Belasco JG

更新日期：2009-04-01 00:00:00

abstract：:A 60 kbp region of the Bacillus subtilis chromosome encompassing the genes concerned with teichoic acid biosynthesis has been subjected to physical analysis. No homology was detected by Southern hybridization between DNA segments encoding the tag genes of strain 168, concerned with polyglycerol phosphate (poly(groP)) ...

journal_title：Molecular microbiology

authors： Young M,Mauël C,Margot P,Karamata D

更新日期：1989-12-01 00:00:00

abstract：:To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expre...

journal_title：Molecular microbiology

authors： Wells JM,Wilson PW,Norton PM,Gasson MJ,Le Page RW

更新日期：1993-06-01 00:00:00

abstract：:Pseudomonas aeruginosa, a Gram-negative bacterium, is a significant opportunistic pathogen associated with skin and soft tissue infections, nosocomial pneumonia and sepsis. In addition, it can chronically colonize the lungs of cystic fibrosis (CF) patients. Overproduction of the exopolysaccharide called alginate provi...

journal_title：Molecular microbiology

authors： Damron FH,Goldberg JB

更新日期：2012-05-01 00:00:00

abstract：:Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or...

journal_title：Molecular microbiology

authors： Rozalski A,Brade L,Kosma P,Moxon R,Kusumoto S,Brade H

更新日期：1997-02-01 00:00:00

abstract：:Two papers in this issue of Molecular Microbiology from Carol Gross and colleagues describe experiments that investigate how two alternative Escherichia coli sigma proteins recognize target promoter -10 regions. A combination of genetics, biochemistry and bioinformatics is used to show that determinants in two adjacen...

journal_title：Molecular microbiology

authors： Busby SJ

更新日期：2009-05-01 00:00:00

abstract：:MsDef1 and MtDef4 from Medicago spp. are small cysteine-rich defensins with potent antifungal activity against a broad range of filamentous fungi. Each defensin has a hallmark γ-core motif (GXCX(3-9) C), which contains major determinants of its antifungal activity. In this study, the antifungal activities of MsDef1, M...

journal_title：Molecular microbiology

authors： Muñoz A,Chu M,Marris PI,Sagaram US,Kaur J,Shah DM,Read ND

更新日期：2014-06-01 00:00:00

abstract：:Bacterial ribosome requires elongation factor P to translate fragments harbouring consecutive proline codons. Given the abundance of ORFs with potential EF-P regulated sites, EF-P was assumed to be constitutively expressed. Here, we report that the intracellular pathogen Salmonella enterica serovar Typhimurium decreas...

journal_title：Molecular microbiology

authors： Nam D,Choi E,Shin D,Lee EJ

更新日期：2016-10-01 00:00:00

abstract：:Acetyl-coenzyme A synthetase (Acs) activates acetate into acetyl-coenzyme A (Ac-CoA) in most cells. In Salmonella enterica, acs expression and Acs activity are controlled. It is unclear why the sirtuin-dependent protein acylation/deacylation system (SDPADS) controls the activity of Acs. Here we show that, during growt...

journal_title：Molecular microbiology

authors： Chan CH,Garrity J,Crosby HA,Escalante-Semerena JC

更新日期：2011-04-01 00:00:00

abstract：:Mycelial fungi play a central role in element cycling in nature by degrading dead organic material such as wood. Fungal colonization of a substrate starts with the invasion of exploring hyphae. These hyphae secrete enzymes that convert the organic material into small molecules that can be taken up by the fungus to ser...

journal_title：Molecular microbiology

authors： Vinck A,Terlou M,Pestman WR,Martens EP,Ram AF,van den Hondel CA,Wösten HA

更新日期：2005-11-01 00:00:00

abstract：:Gene expression in fungi by ambient pH is regulated via a conserved signalling cascade whose terminal component is the zinc finger transcription factor PacC/Rim1p. We have identified a pacC orthologue in the vascular wilt pathogen Fusarium oxysporum that binds the consensus 5'-GCCAAG-3' sequence and is proteolytically...

journal_title：Molecular microbiology

authors： Caracuel Z,Roncero MI,Espeso EA,González-Verdejo CI,García-Maceira FI,Di Pietro A

更新日期：2003-05-01 00:00:00

abstract：:Misfolded proteins are usually arrested in the endoplasmic reticulum (ER) and degraded by the ER-associated degradation (ERAD) machinery. Several mutant alleles of PMA1, the gene coding for the plasma membrane H(+)-ATPase, render misfolded proteins that are retained in the ER and degraded by ERAD. A subset of misfolde...

journal_title：Molecular microbiology

authors： Mazón MJ,Eraso P,Portillo F

更新日期：2007-02-01 00:00:00

abstract：:Bacteria attach to their appropriate environmental niche by using adhesins. To maximize their contact with the environment, adhesins are often present on the ends of long hairlike structures called pili. Recently, attention has focused on pili of Gram-positive bacteria because they may be vaccine candidates in importa...

journal_title：Molecular microbiology

authors： Scott JR,Zähner D

更新日期：2006-10-01 00:00:00

abstract：:XylR is a σ⁵⁴-dependent transcriptional factor of Pseudomonas putida that activates the Pu promoter of the TOL plasmid upon binding its natural effector, m-xylene. The search for mutants of the signal-sensing module of XylR that respond to the xenobiotic compound 2,4-dinitrotoluene recurrently yields protein variants ...

journal_title：Molecular microbiology

authors： de Las Heras A,de Lorenzo V

更新日期：2011-03-01 00:00:00

abstract：:Photorhabdus and Xenorhabdus bacteria colonize the intestines of the infective soil-dwelling stage of entomophagous nematodes, Heterorhabditis and Steinernema, respectively. These nematodes infect susceptible insect larvae and release the bacteria into the insect blood. The bacteria kill the insect larvae and convert ...

journal_title：Molecular microbiology

authors： Goodrich-Blair H,Clarke DJ

更新日期：2007-04-01 00:00:00

abstract：:To establish a lysogenic lifestyle, the temperate bacteriophage φC31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site). This reaction is promoted by a phage-encoded serine rec...

journal_title：Molecular microbiology

authors： Stark WM

更新日期：2011-06-01 00:00:00

abstract：:Fungal ribotoxins, such as mitogillin and the related Aspergillus toxins restrictocin and alpha-sarcin, are highly specific ribonucleases, which inactivate the ribosome enzymatically by cleaving the eukaryotic 28S RNA of the large ribosomal subunit at a single phosphodiester bond. The site of cleavage occurs between G...

journal_title：Molecular microbiology

authors： Kao R,Shea JE,Davies J,Holden DW

更新日期：1998-08-01 00:00:00

abstract：:In cell-free Yersinia pseudotuberculosis culture supernatants, we have chemically characterized three N-acyl homoserine lactone (AHL) molecules, N-octanoyl homoserine lactone (C8-HSL), N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6-HSL) and N-hexanoyl homoserine lactone (C6-HSL). We have identified, cloned and sequence...

journal_title：Molecular microbiology

authors： Atkinson S,Throup JP,Stewart GS,Williams P

更新日期：1999-09-01 00:00:00

abstract：:The virulent phenotype of Shigella requires loci on the chromosome as well as on the large virulence plasmid, and is regulated via a complex web of interactions amongst various chromosomal and large plasmid genes. To further investigate the role of chromosomal loci in virulence, we performed random Tn10 mutagenesis in...

journal_title：Molecular microbiology

authors： Mac Síomóin RA,Nakata N,Murai T,Yoshikawa M,Tsuji H,Sasakawa C

更新日期：1996-02-01 00:00:00

abstract：:Transcription of lysine genes in Saccharomyces cerevisiae is dependent on Lys14p and on alpha-aminoadipate semialdehyde (alphaAASA), an intermediate of the pathway. The two-thirds C-terminal end of Lys14p is sufficient to ensure the activation function of the protein and its modulation by alphaAASA. Here, we show that...

journal_title：Molecular microbiology

authors： El Alami M,Feller A,Piérard A,Dubois E

更新日期：2002-03-01 00:00:00

abstract：:Bacteria of Shigella spp. are responsible for shigellosis in humans. They use a type III secretion (TTS) system encoded by a 200 kb virulence plasmid to enter epithelial cells and trigger apoptosis in macrophages. This TTS system comprises a secretion apparatus, translocators and effectors that transit through this ap...

journal_title：Molecular microbiology

authors： Penno C,Sansonetti P,Parsot C

更新日期：2005-04-01 00:00:00

abstract：:Bacterial cytochrome cbb3 oxidases are members of the haeme-copper oxidase superfamily that are important for energy conservation by a variety of proteobacteria under oxygen-limiting conditions. The opportunistic pathogen Pseudomonas aeruginosa is unusual in possessing two operons that each potentially encode a cbb3 o...

journal_title：Molecular microbiology

authors： Comolli JC,Donohue TJ

更新日期：2004-02-01 00:00:00

abstract：:Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...

journal_title：Molecular microbiology

authors： Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

更新日期：2002-11-01 00:00:00

abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...

journal_title：Molecular microbiology

authors： Veith A,Klingl A,Zolghadr B,Lauber K,Mentele R,Lottspeich F,Rachel R,Albers SV,Kletzin A

更新日期：2009-07-01 00:00:00

abstract：:The hok/sok locus of plasmid R1 mediates plasmid stabilization by the killing of plasmid-free cells. Many bacterial plasmids carry similar loci. For example, the F plasmid carries two hok homologues, flm and srnB, that mediate plasmid stabilization by this specialized type of programmed cell death. Here, we show that ...

journal_title：Molecular microbiology

authors： Pedersen K,Gerdes K

更新日期：1999-06-01 00:00:00

abstract：:The exosporium is the outermost layer of the Bacillus anthracis spore. The predominant protein on the exosporium surface is BclA, a collagen-like glycoprotein. BclA is incorporated on the spore surface late in the B. anthracis sporulation pathway. A second collagen-like protein, BclB, has been shown to be surface-expo...

journal_title：Molecular microbiology

authors： Thompson BM,Stewart GC

更新日期：2008-10-01 00:00:00