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tRNAPro -mediated downregulation of elongation factor P is required for mgtCBR expression during Salmonella infection.

Abstract：

:Bacterial ribosome requires elongation factor P to translate fragments harbouring consecutive proline codons. Given the abundance of ORFs with potential EF-P regulated sites, EF-P was assumed to be constitutively expressed. Here, we report that the intracellular pathogen Salmonella enterica serovar Typhimurium decreases efp mRNA levels during course of infection. We determined that the decrease in efp mRNA is triggered by low levels of charged tRNAPro , a condition that Salmonella experiences when inside a macrophage phagosome. Surprisingly, downregulation of EF-P selectively promotes expression of the virulence mgtC gene and contributes to Salmonella's ability to survive inside macrophages. The decrease in EF-P levels induces ribosome stalling at the consecutive proline codons of the mgtP open reading frame in the mgtCBR leader RNA, and thus allows formation of a stem-loop structure promoting transcription of the mgtC gene. The substitution of proline codons in the mgtP gene eliminates EF-P-mediated mgtC expression and thus Salmonella's survival inside macrophages. Our findings indicate that Salmonella benefits virulence genes by decreasing EF-P levels and inducing the stringent response inside host.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Nam D,Choi E,Shin D,Lee EJ

doi

10.1111/mmi.13454

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

221-232

issue

2

eissn

0950-382X

issn

1365-2958

journal_volume

102

pub_type

杂志文章

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