Abstract:
:Pseudomonas aeruginosa releases several extracellular proteins which are secreted via two independent secretion pathways. Alkaline protease (AprA) Is released by its own specific secretion machinery which is an ABC-transporter. Despite sequence similarities between components of ABC-transporters in different bacteria, each transporter is dedicated to the secretion of a particular protein or a family of closely related proteins. Heterologous complementation between ABC-transporters for unrelated polypeptides can occur, but only at a very low level. We show that the 50 C-terminal amino acids of AprA constitute an autonomous secretion signal. By heterologous complementation experiments between the unrelated alpha-haemolysin (HlyA) and Apr secretion systems we demonstrated that it is only the recognition of the secretion signal by the translocator which confers specificity to the secretion process. Secretion was size-dependent. However inclusion of glycine-rich repeats from HlyA in AprA seems to overcome the size limitation exerted by the Apr secretion apparatus such that the machinery secreted a hybrid protein 20 kDa larger than the normal maximal size.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Duong F,Lazdunski A,Murgier Mdoi
10.1111/j.1365-2958.1996.tb02555.xsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
459-70issue
3eissn
0950-382Xissn
1365-2958journal_volume
21pub_type
杂志文章abstract::A segment of the exotoxin A gene of Pseudomonas aeruginosa, coding for the N-terminal end of domain I and domain II of the toxin (ETA), was genetically fused to the diphtheria toxin gene of Corynebacterium diphtheriae, coding for the N-terminal end of A fragment of diphtheria toxin (DT). The resulting hybrid protein (...
journal_title:Molecular microbiology
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pub_type: 评论,杂志文章
doi:
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更新日期:2020-04-01 00:00:00
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更新日期:1999-07-01 00:00:00
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更新日期:2009-01-01 00:00:00
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更新日期:2000-05-01 00:00:00
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更新日期:2016-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
abstract::The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of antigenically diverse proteins is expressed on the surface of human erythrocytes infected with the malaria parasite P. falciparum, and mediates cytoadherence to the host vascular endothelium. In this report, we show that export of PfEMP1 is sl...
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