A rapid method for reconstitution of bacterial membrane proteins.

abstract：:In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15-acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host...

journal_title：Molecular microbiology

authors： Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

更新日期：2020-10-01 00:00:00

abstract：:Cell-cell communication via the production and detection of chemical signal molecules has been the focus of a great deal of research over the past decade. One class of chemical signals widely used by proteobacteria consists of N-acyl-homoserine lactones, which are synthesized by proteins related to LuxI of Vibrio fisc...

journal_title：Molecular microbiology

authors： Pappas KM,Weingart CL,Winans SC

更新日期：2004-08-01 00:00:00

abstract：:The localization of SeqA, thymidylate synthase, DnaB (helicase) and the DNA polymerase components alpha and tau, has been studied by immunofluorescence microscopy. The origin has been labelled through GFP-LacI bound near oriC. SeqA was located in the cell centre for one replication factory (RF) and at 1/4 and 3/4 posi...

journal_title：Molecular microbiology

authors： den Blaauwen T,Aarsman ME,Wheeler LJ,Nanninga N

更新日期：2006-11-01 00:00:00

abstract：:AtzR is a LysR-type regulator responsible for activation of the cyanuric acid utilization operon atzDEF. AtzR binds the PatzDEF promoter region at a strong recognition element, designated the repressor binding site, and a weaker binding determinant, the activator binding site (ABS). AtzR activates transcription in res...

journal_title：Molecular microbiology

authors： Porrúa O,Platero AI,Santero E,Del Solar G,Govantes F

更新日期：2010-04-01 00:00:00

abstract：:Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus. The VirB membrane proteins are postulated to form a pore for the transport of the DNA across the bacterial membranes. Immunofluorescence and immunoelectron microscopy were used to study the transport pore complex. Three likely c...

journal_title：Molecular microbiology

authors： Kumar RB,Xie YH,Das A

更新日期：2000-05-01 00:00:00

abstract：:The non-essential RGD1 gene from Saccharomyces cerevisiae encodes a protein that has been characterized in vitro as a Rho GTPase activating protein (RhoGAP) for the Rho3 and Rho4 proteins. Rgd1p, which displays a conserved FCH-coiled coil-Rho-GAP domain organization, showed a patch-like distribution in the cell, inclu...

journal_title：Molecular microbiology

authors： Roumanie O,Peypouquet MF,Bonneu M,Thoraval D,Doignon F,Crouzet M

更新日期：2000-06-01 00:00:00

abstract：:In response to recommendations from the Steering Committees responsible for co-ordination of World Health Organization programmes for research on the immunology of leprosy (IMMLEP) and tuberculosis (IMMTUB), a list was prepared summarizing the properties of mycobacterial proteins currently under investigation with res...

journal_title：Molecular microbiology

authors： Young DB,Kaufmann SH,Hermans PW,Thole JE

更新日期：1992-01-01 00:00:00

abstract：:We cloned the CaYFH1 gene that encodes the yeast frataxin homologue in Candida albicans. CaYFH1 was expressed in Deltayfh1 Saccharomyces cerevisiae cells, where it compensated for all the phenotypes tested except for the lack of cytochromes. Double DeltaCayfh1/DeltaCayfh1 mutant had severe defective growth, accumulate...

journal_title：Molecular microbiology

authors： Santos R,Buisson N,Knight SA,Dancis A,Camadro JM,Lesuisse E

更新日期：2004-10-01 00:00:00

abstract：:Oxidative stress serves as an important host/environmental signal that triggers a wide range of responses from the human pathogen Staphylococcus aureus. Among these, a thiol-based oxidation sensing pathway through a global regulator MgrA controls the virulence and antibiotic resistance of the bacterium. Herein, we rep...

journal_title：Molecular microbiology

authors： Chen PR,Nishida S,Poor CB,Cheng A,Bae T,Kuechenmeister L,Dunman PM,Missiakas D,He C

更新日期：2009-01-01 00:00:00

abstract：:When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtil...

journal_title：Molecular microbiology

authors： Wiegert T,Homuth G,Versteeg S,Schumann W

更新日期：2001-07-01 00:00:00

abstract：:Myxococcus xanthus is a Gram-negative, soil-dwelling bacterium with a complex life cycle which includes fruiting body formation and sporulation in response to starvation. This developmental process is slow, requiring a minimum of 24-48 h, and requires cells to be at high cell density on a solid surface. It is known th...

journal_title：Molecular microbiology

authors： O'Connor KA,Zusman DR

更新日期：1997-05-01 00:00:00

abstract：:The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine th...

journal_title：Molecular microbiology

authors： Parveen N,Cornell KA

更新日期：2011-01-01 00:00:00

abstract：:We determined the genome-wide environmental stress response (ESR) expression profile of Candida glabrata, a human pathogen related to Saccharomyces cerevisiae. Despite different habitats, C. glabrata, S. cerevisiae, Schizosaccharomyces pombe and Candida albicans have a qualitatively similar ESR. We investigate the fun...

journal_title：Molecular microbiology

authors： Roetzer A,Gregori C,Jennings AM,Quintin J,Ferrandon D,Butler G,Kuchler K,Ammerer G,Schüller C

更新日期：2008-08-01 00:00:00

abstract：:Arginine auxotrophs of the dinitrogen-fixing cyanobacterium Anabaena species strain PCC 7120 were isolated after ultraviolet light mutagenesis and penicillin enrichment. Two of these auxotrophs were complemented by a cosmid gene library of the wild-type strain established in Escherichia coli that was transferred en ma...

journal_title：Molecular microbiology

authors： Floriano B,Herrero A,Flores E

更新日期：1992-08-01 00:00:00

abstract：:The pathogenic fungus Candida albicans commonly causes mucosal surface infections. In immunocompromised patients, C. albicans may penetrate into deeper tissue, enter the bloodstream and disseminate within the host causing life-threatening systemic infections. In order to elucidate how C. albicans responds to the chall...

journal_title：Molecular microbiology

authors： Fradin C,Kretschmar M,Nichterlein T,Gaillardin C,d'Enfert C,Hube B

更新日期：2003-03-01 00:00:00

abstract：:We describe a chemotactic-like response of Escherichia coli strains lacking most of the known chemotaxis machinery but containing high levels of the response regulator CheY. The bacteria accumulated in aspartate-containing capillaries, they formed rings on tryptone-containing semisolid agar, and the probability of cou...

journal_title：Molecular microbiology

authors： Barak R,Eisenbach M

更新日期：1999-02-01 00:00:00

abstract：:A recurring theme in morphogenesis is the coupling of the expression of genes that drive morphogenesis and the morphogenetic process per se. This coupling ensures that gene expression and morphogenesis are carried out in synchrony. Morphogenesis of the spore-filled fruiting bodies in Myxococcus xanthus illustrates thi...

journal_title：Molecular microbiology

authors： Søgaard-Andersen L,Overgaard M,Lobedanz S,Ellehauge E,Jelsbak L,Rasmussen AA

更新日期：2003-04-01 00:00:00

abstract：:To study the role of cell division in the process of nucleoid segregation, we measured the DNA content of individual nucleoids in isogenic Escherichia coli cell division mutants by image cytometry. In pbpB(Ts) and ftsZ strains growing as filaments at 42 degrees C, nucleoids contained, on average, more than two chromos...

journal_title：Molecular microbiology

authors： Huls PG,Vischer NO,Woldringh CL

更新日期：1999-09-01 00:00:00

abstract：:A novel two-component system (TCS) designated as DraR-K (sco3063/sco3062) was identified to be involved in differential regulation of antibiotic biosynthesis in Streptomyces coelicolor. The S. coelicolor mutants with deletion of either or both of draR and draK exhibited significantly reduced actinorhodin (ACT) but inc...

journal_title：Molecular microbiology

authors： Yu Z,Zhu H,Dang F,Zhang W,Qin Z,Yang S,Tan H,Lu Y,Jiang W

更新日期：2012-08-01 00:00:00

abstract：:The Escherichia coli Sm-like host factor I (Hfq) is thought to play direct and indirect roles in post-transcriptional regulation by targeting small regulatory RNAs and mRNAs. In this study, we have used proteomics to identify new mRNA targets of Hfq. We have identified 11 candidate proteins, synthesis of which was dif...

journal_title：Molecular microbiology

authors： Vecerek B,Moll I,Afonyushkin T,Kaberdin V,Bläsi U

更新日期：2003-11-01 00:00:00

abstract：:The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a 'bound-form', which is covalently bound to the cell's peptidoglycan layer, ...

journal_title：Molecular microbiology

authors： Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

更新日期：2011-03-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:Disulfide bonds are found in many proteins associated with the cell wall of Escherichia coli, and for some of these proteins the disulfide bond is critical to their stability and function. One protein found to contain a disulfide bond is the essential cell division protein FtsN, but the importance of this bond to the ...

journal_title：Molecular microbiology

authors： Meehan BM,Landeta C,Boyd D,Beckwith J

更新日期：2017-02-01 00:00:00

abstract：:Bacillus subtilis SpoIIE is a Ser protein phosphatase whose action on the phosphoprotein SpoIIAA triggers the cell type-specific activation of a sporulation transcription factor. Here we report that SpoIIE displays sequence similarity to the PP2C family of eukaryotic Ser/Thr protein phosphatases, and that residues com...

journal_title：Molecular microbiology

authors： Adler E,Donella-Deana A,Arigoni F,Pinna LA,Stragler P

更新日期：1997-01-01 00:00:00

abstract：:Accumulation of stalled ribosomes at the 3' end of mRNA without a stop codon (non-stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescue such stalled ribosomes: SsrA-dependent trans-translation and ArfA-dependent ribosome rescue. Combination of the ssrA and ...

journal_title：Molecular microbiology

authors： Chadani Y,Ono K,Kutsukake K,Abo T

更新日期：2011-05-01 00:00:00

abstract：:The bacterial flagellum contains a specialized secretion apparatus in its base that pumps certain protein subunits through the growing structure to their sites of installation beyond the membrane. A related apparatus functions in the injectisomes of gram-negative pathogens to export virulence factors into host cells. ...

journal_title：Molecular microbiology

authors： Erhardt M,Wheatley P,Kim EA,Hirano T,Zhang Y,Sarkar MK,Hughes KT,Blair DF

更新日期：2017-04-01 00:00:00

abstract：:In Saccharomyces cerevisiae MATa cells, export of the a-factor mating pheromone is mediated by Ste6p, a member of the ATP-binding cassette (ABC) superfamily of transporters and a close homologue of mammalian multidrug transporter P-glycoproteins (Pgps). We have used functional complementation of a ste6delta mutation t...

journal_title：Molecular microbiology

authors： Raymond M,Dignard D,Alarco AM,Mainville N,Magee BB,Thomas DY

更新日期：1998-02-01 00:00:00

abstract：:Sensory adaptation of low-abundance chemoreceptors in Escherichia coli requires assistance from high-abundance receptors, because only high-abundance receptors carry the carboxyl-terminal pentapeptide sequence NWETF that enhances adaptational covalent modification. Using membrane vesicles containing both high-abundanc...

journal_title：Molecular microbiology

authors： Li M,Hazelbauer GL

更新日期：2005-06-01 00:00:00

abstract：:Escherichia coli K-12 strain PS1-28-37 carries the multicopy plasmid pPSO28-37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and pur...

journal_title：Molecular microbiology

authors： Schülein K,Schmid K,Benzl R

更新日期：1991-09-01 00:00:00

abstract：:Exposure of bacterial cells to temperature changes induces the synthesis of a set proteins. We investigated the control of expression of the cspA gene, coding for the major cold-shock protein of Escherichia coli. This protein was shown to be transiently induced upon shift to low temperature. We demonstrated that the c...

journal_title：Molecular microbiology

authors： Goldenberg D,Azar I,Oppenheim AB

更新日期：1996-01-01 00:00:00