Isolation of arginine auxotrophs, cloning by mutant complementation, and sequence analysis of the argC gene from the cyanobacterium Anabaena species PCC 7120.

abstract：:Sigma28 controls the expression of flagella-related genes and is the most widely distributed alternative sigma factor, present in motile Gram-positive and Gram-negative bacteria. The distinguishing feature of sigma28 promoters is a long -10 region (GCCGATAA). Despite the fact that the upstream GC is highly conserved, ...

journal_title：Molecular microbiology

authors： Koo BM,Rhodius VA,Campbell EA,Gross CA

更新日期：2009-05-01 00:00:00

abstract：:The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green f...

journal_title：Molecular microbiology

authors： Bryan SJ,Burroughs NJ,Shevela D,Yu J,Rupprecht E,Liu LN,Mastroianni G,Xue Q,Llorente-Garcia I,Leake MC,Eichacker LA,Schneider D,Nixon PJ,Mullineaux CW

更新日期：2014-10-13 00:00:00

abstract：:The chromosome of Escherichia coli K-12 contains a putative gene, yheB (chiA), at centisome 74.7, whose product shows sequence similarity with chitinases of bacterial and viral origin. We cloned the chiA (yheB) gene and demonstrated that it codes for a 94.5 kDa periplasmic protein with endochitinase/lysozyme activity....

journal_title：Molecular microbiology

authors： Francetic O,Badaut C,Rimsky S,Pugsley AP

更新日期：2000-03-01 00:00:00

abstract：:The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiom...

journal_title：Molecular microbiology

authors： Hardy SJ,Christodoulides M,Weller RO,Heckels JE

更新日期：2000-05-01 00:00:00

abstract：:Phycobilisomes are the major constituents of the light-harvesting apparatus in both cyanobacteria and red algae and consist of a central core with radiating rods. From a genomic library of the cyanobacterium Calothrix 7601, a DNA fragment encoding allophycocyanin B, one of the two terminal energy acceptors of the core...

journal_title：Molecular microbiology

authors： Houmard J,Capuano V,Coursin T,Tandean de Marsac N

更新日期：1988-01-01 00:00:00

abstract：:Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of ...

journal_title：Molecular microbiology

authors： Monack DM,Arico B,Rappuoli R,Falkow S

更新日期：1989-12-01 00:00:00

abstract：:The protozoan parasite Trypanosoma brucei develops antigenic variation to escape the immune response of its host. To this end, the trypanosome genome contains multiple telomeric expression sites competent for transcription of variant surface glycoprotein genes, but as a rule only a single antigen is expressed at any t...

journal_title：Molecular microbiology

authors： Vanhamme L,Poelvoorde P,Pays A,Tebabi P,Van Xong H,Pays E

更新日期：2000-04-01 00:00:00

abstract：:The efficiency of beta-lactam antibiotics, which are among our most useful chemotherapeutic weapons, is continuously challenged by the emergence of resistant bacterial strains. This is most often due to the production of beta-lactamases by the resistant cells. These enzymes inactivate the antibiotics by hydrolysing th...

journal_title：Molecular microbiology

authors： Frère JM

更新日期：1995-05-01 00:00:00

abstract：:In contrast to many antimicrobial peptides, members of the proline-rich group of antimicrobial peptides inactivate Gram-negative bacteria by a non-lytic mechanism. Several lines of evidence indicate that they are internalized into bacteria and their activity mediated by interaction with unknown cellular components. Wi...

journal_title：Molecular microbiology

authors： Mattiuzzo M,Bandiera A,Gennaro R,Benincasa M,Pacor S,Antcheva N,Scocchi M

更新日期：2007-10-01 00:00:00

abstract：:9 alpha-Hydroxylation of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial steroid catabolism. Very limited knowledge is presently available on the KSH enzyme. Here, we report for the first time the identification and ...

journal_title：Molecular microbiology

authors： van der Geize R,Hessels GI,van Gerwen R,van der Meijden P,Dijkhuizen L

更新日期：2002-08-01 00:00:00

abstract：:Biosynthesis of fatty acids is one of the most fundamental biochemical pathways in nature. In bacteria and plant chloroplasts, the committed and rate-limiting step in fatty acid biosynthesis is catalyzed by a multi-subunit form of the acetyl-CoA carboxylase enzyme (ACC). This enzyme carboxylates acetyl-CoA to produce ...

journal_title：Molecular microbiology

authors： Gerhardt EC,Rodrigues TE,Müller-Santos M,Pedrosa FO,Souza EM,Forchhammer K,Huergo LF

更新日期：2015-03-01 00:00:00

abstract：:The Escherichia coli UvrD helicase (or helicase II) is known for its involvement in DNA repair. We report that UvrD is required for DNA replication of several different rolling-circle plasmids in E. coli, whereas its homologue, the Rep helicase, is not. Lack of UvrD helicase does not impair the first step of plasmid r...

journal_title：Molecular microbiology

authors： Bruand C,Ehrlich SD

更新日期：2000-01-01 00:00:00

abstract：:The hok/sok locus of plasmid R1 mediates plasmid stabilization by the killing of plasmid-free cells. Many bacterial plasmids carry similar loci. For example, the F plasmid carries two hok homologues, flm and srnB, that mediate plasmid stabilization by this specialized type of programmed cell death. Here, we show that ...

journal_title：Molecular microbiology

authors： Pedersen K,Gerdes K

更新日期：1999-06-01 00:00:00

abstract：:The Yersinia Ysc apparatus is made of more than 20 proteins, 11 of which have homologues in many type III systems. Here, we characterize YscP from Yersinia enterocolitica. This 515-residue protein has a high proline content, a large tandem repetition and a slow migration in SDS-PAGE. Unlike the products of neighbourin...

journal_title：Molecular microbiology

authors： Stainier I,Bleves S,Josenhans C,Karmani L,Kerbourch C,Lambermont I,Tötemeyer S,Boyd A,Cornelis GR

更新日期：2000-09-01 00:00:00

abstract：:Interactions between domains of ATP-binding cassette (ABC) transporters are of great functional importance and yet are poorly understood. To gain further knowledge of these protein-protein interactions, we studied the inner membrane complex of the maltose transporter of Escherichia coli. We focused on interactions bet...

journal_title：Molecular microbiology

authors： Mourez M,Jéhanno M,Schneider E,Dassa E

更新日期：1998-10-01 00:00:00

abstract：:Bacteria can respond to a variety of environmental stimuli by means of systems generally composed of two proteins. The first protein (sensor or transmitter) is usually a transmembrane protein with cytoplasmic and extracytoplasmic domains. The extracytoplasmic domain (sensor) senses the environment and transfers the si...

journal_title：Molecular microbiology

authors： Gross R,Aricò B,Rappuoli R

更新日期：1989-11-01 00:00:00

abstract：:The unfolded protein response (UPR) is an important stress signalling pathway involved in the cellular development and environmental adaptation of fungi. We investigated the importance of the UPR pathway in the pathogenicity of the plant necrotrophic fungus Alternaria brassicicola, which causes black spot disease on a...

journal_title：Molecular microbiology

authors： Joubert A,Simoneau P,Campion C,Bataillé-Simoneau N,Iacomi-Vasilescu B,Poupard P,François JM,Georgeault S,Sellier E,Guillemette T

更新日期：2011-03-01 00:00:00

abstract：:Aspergillus nidulans was used as a model organism to investigate the fungal propionate metabolism and the mechanism of growth inhibition by propionate. The fungus is able to grow slowly on propionate as sole carbon and energy source. Propionate is oxidized to pyruvate via the methylcitrate cycle. The key enzyme methyl...

journal_title：Molecular microbiology

authors： Brock M,Fischer R,Linder D,Buckel W

更新日期：2000-03-01 00:00:00

abstract：:The Ca(2+)-dependent protein phosphatase calcineurin is an important regulator of ion transporters from many organisms, including the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger Vcx1p. In yeast and plants, cation/H(+) exchangers are important in shaping cytosolic Ca(2+) levels involved in signal transducti...

journal_title：Molecular microbiology

authors： Pittman JK,Cheng NH,Shigaki T,Kunta M,Hirschi KD

更新日期：2004-11-01 00:00:00

abstract：:A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of PfHT as a novel drug target. Following recent re-annotations of the P....

journal_title：Molecular microbiology

authors： Slavic K,Straschil U,Reininger L,Doerig C,Morin C,Tewari R,Krishna S

更新日期：2010-03-01 00:00:00

abstract：:Cpmk2, encoding a mitogen-activated protein (MAP) kinase from the ascomycete Claviceps purpurea, is an orthologue of SLT2 from Saccharomyces cerevisiae, the first isolated from a biotrophic, non-appressorium-forming pathogen. Deletion mutants obtained by a gene replacement approach show impaired vegetative properties ...

journal_title：Molecular microbiology

authors： Mey G,Held K,Scheffer J,Tenberge KB,Tudzynski P

更新日期：2002-10-01 00:00:00

abstract：:The archaeal transcription apparatus is chimeric: its core components (RNA polymerase and basal factors) closely resemble those of eukaryotic RNA polymerase II, but the putative archaeal transcriptional regulators are overwhelmingly of bacterial type. Particular interest attaches to how these bacterial-type effectors,...

journal_title：Molecular microbiology

authors： Ouhammouch M,Langham GE,Hausner W,Simpson AJ,El-Sayed NM,Geiduschek EP

更新日期：2005-05-01 00:00:00

abstract：:We have isolated and sequenced a set of deletions stimulated by DNA palindromes in Escherichia coli. All of the deletions are asymmetric with respect to the parental sequence and have occurred at short direct repeats. This is consistent with deletion by strand slippage during DNA replication. The orientation of the as...

journal_title：Molecular microbiology

authors： Pinder DJ,Blake CE,Lindsey JC,Leach DR

更新日期：1998-05-01 00:00:00

abstract：:The cGvpE protein of Halobacterium salinarum PHH4 has been identified as transcriptional activator for the promoter of the c-gvpA gene encoding the major gas vesicle structural protein cGvpA. Molecular modelling of the carboxy-terminal region of cGvpE suggests that this protein resembles a basic leucine-zipper protein...

journal_title：Molecular microbiology

authors： Plösser P,Pfeifer F

更新日期：2002-07-01 00:00:00

abstract：:Structural maintenance of chromosome (SMC) and the SMC-interacting kleisin protein families have key functions in the chromosome organization of most organisms. Here, we report that the Bacillus subtilis kleisin, ScpA, can form a ternary complex with the SMC and ScpB proteins in a yeast tri-hybrid assay, supporting th...

journal_title：Molecular microbiology

authors： Dervyn E,Noirot-Gros MF,Mervelet P,McGovern S,Ehrlich SD,Polard P,Noirot P

更新日期：2004-03-01 00:00:00

abstract：:Phospholipid homeostasis of the bacterial membrane is maintained by biochemical regulation of the synthesis enzymes depending on the environment. However, genes encoding phospholipid synthesis enzymes might also be regulated during stress responses, in order for the bacteria to adapt their growth to changing environme...

journal_title：Molecular microbiology

authors： Wahl A,My L,Dumoulin R,Sturgis JN,Bouveret E

更新日期：2011-06-01 00:00:00

abstract：:The proton-driven flagellar motor of Salmonella enterica can accommodate a dozen MotA/B stators in a load-dependent manner. The C-terminal periplasmic domain of MotB acts as a structural switch to regulate the number of active stators in the motor in response to load change. The cytoplasmic loop termed MotAC is respon...

journal_title：Molecular microbiology

authors： Pourjaberi SNS,Terahara N,Namba K,Minamino T

更新日期：2017-11-01 00:00:00

abstract：:Multimer formation reduces plasmid copy number and is an established cause of segregational instability. Nevertheless, it is difficult to rationalize observations that low levels of dimers can cause severe instability, if we assume they are distributed evenly in cell populations. We report here that dimer distribution...

journal_title：Molecular microbiology

authors： Summers DK,Beton CW,Withers HL

更新日期：1993-06-01 00:00:00

abstract：:We report selective phosphorylation of the DNA-binding domain of the Streptococcus pneumoniae transcriptional regulator RitR. RitR is annotated as a two-component response regulator, but lacks a cognate His kinase as a neighbouring locus in the genome. In addition, Asn replaces Asp at the expected acceptor site. By th...

journal_title：Molecular microbiology

authors： Ulijasz AT,Falk SP,Weisblum B

更新日期：2009-01-01 00:00:00

abstract：:Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis, is embedded in the plasma membrane bilayer via four transmembrane segments TM1-TM4 that form...

journal_title：Molecular microbiology

authors： Hardt K,Joris B,Lepage S,Brasseur R,Lampen JO,Frère JM,Fink AL,Ghuysen JM

更新日期：1997-03-01 00:00:00