Abstract:
:Ribosome-tethered chaperones that interact with nascent polypeptide chains have been identified in both prokaryotic and eukaryotic systems. However, these ribosome-associated chaperones share no sequence similarity: bacterial trigger factors (TF) form an independent protein family while the yeast machinery is Hsp70-based. The absence of any component of the yeast machinery results in slow growth at low temperatures and sensitivity to aminoglycoside protein synthesis inhibitors. After establishing that yeast ribosomal protein Rpl25 is able to recruit TF to ribosomes when expressed in place of its Escherichia coli homologue L23, the ribosomal TF tether, we tested whether such divergent ribosome-associated chaperones are functionally interchangeable. E. coli TF was expressed in yeast cells that lacked the endogenous ribosome-bound machinery. TF associated with yeast ribosomes, cross-linked to yeast nascent polypeptides and partially complemented the aminoglycoside sensitivity, demonstrating that ribosome-associated chaperones from divergent organisms share common functions, despite their lack of sequence similarity.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Rauch T,Hundley HA,Pfund C,Wegrzyn RD,Walter W,Kramer G,Kim SY,Craig EA,Deuerling Edoi
10.1111/j.1365-2958.2005.04690.xsubject
Has Abstractpub_date
2005-07-01 00:00:00pages
357-65issue
2eissn
0950-382Xissn
1365-2958pii
MMI4690journal_volume
57pub_type
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