Dissecting functional similarities of ribosome-associated chaperones from Saccharomyces cerevisiae and Escherichia coli.

abstract：:We recently described the use of selective transposon mutagenesis to generate a series of avirulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly ho...

journal_title：Molecular microbiology

authors： Johnson K,Charles I,Dougan G,Pickard D,O'Gaora P,Costa G,Ali T,Miller I,Hormaeche C

更新日期：1991-02-01 00:00:00

abstract：:Ureaplasma urealyticum (U. urealyticum), belonging to the class Mollicutes, is a human pathogen colonizing the urogenital tract and causes among other things respiratory diseases in premature infants. We have studied the salvage of pyrimidine deoxynucleosides in U. urealyticum and cloned a key salvage enzyme, thymidin...

journal_title：Molecular microbiology

authors： Carnrot C,Wehelie R,Eriksson S,Bölske G,Wang L

更新日期：2003-11-01 00:00:00

abstract：:The Escherichia coli UvrD helicase (or helicase II) is known for its involvement in DNA repair. We report that UvrD is required for DNA replication of several different rolling-circle plasmids in E. coli, whereas its homologue, the Rep helicase, is not. Lack of UvrD helicase does not impair the first step of plasmid r...

journal_title：Molecular microbiology

authors： Bruand C,Ehrlich SD

更新日期：2000-01-01 00:00:00

abstract：:Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs show a great variability in their shapes, sizes, and location on the particle. Some RBPs are known to depolymerize surface polysaccharides of...

journal_title：Molecular microbiology

authors： Prokhorov NS,Riccio C,Zdorovenko EL,Shneider MM,Browning C,Knirel YA,Leiman PG,Letarov AV

更新日期：2017-08-01 00:00:00

abstract：:The minimum time (E) required for a new pair of replication origins (oriCs) produced upon initiating a round of replication to be ready to initiate the next round after one cell mass doubling, the 'eclipse', is explained in terms of a minimal distance (l(min)) that the replication forks must move away from oriC before...

journal_title：Molecular microbiology

authors： Zaritsky A,Vischer N,Rabinovitch A

更新日期：2007-01-01 00:00:00

abstract：:The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of trivalent metalloids, arsenite and antimonite. We have earlier shown that downregulation of LmAQP1 provides resistance to trivalent antimony compounds whereas increased expression of LmAQP1 in drug-resistant parasites can reverse the re...

journal_title：Molecular microbiology

authors： Figarella K,Uzcategui NL,Zhou Y,LeFurgey A,Ouellette M,Bhattacharjee H,Mukhopadhyay R

更新日期：2007-08-01 00:00:00

abstract：:Crs5 is a Saccharomyces cerevisiae Metallothionein (MT), non-homologous to the paradigmatic Cu-thionein Cup1. Although considered a secondary copper-resistance agent, we show here that it determines survival under zinc overload in a CUP1-null background. Its overexpression prevents the deleterious effects exhibited by...

journal_title：Molecular microbiology

authors： Pagani A,Villarreal L,Capdevila M,Atrian S

更新日期：2007-01-01 00:00:00

abstract：:The syrD gene of Pseudomonas syringae pathovar syringae strain B301D-R was characterized and sequenced. The syrD open reading frame is 1695 bp long and encodes a predicted protein, SyrD, of approximately 63 kDa. Database searches revealed that SyrD shares a high degree of similarity with the ATP-binding cassette (ABC)...

journal_title：Molecular microbiology

authors： Quigley NB,Mo YY,Gross DC

更新日期：1993-08-01 00:00:00

abstract：:Biofilm formation of Staphylococcus epidermidis on smooth polymer surfaces has been shown to be mediated by the ica operon. Upon activation of this operon, a polysaccharide intercellular adhesin (PIA) is synthesized that supports bacterial cell-to-cell contacts and triggers the production of thick, multilayered biofil...

journal_title：Molecular microbiology

authors： Ziebuhr W,Krimmer V,Rachid S,Lössner I,Götz F,Hacker J

更新日期：1999-04-01 00:00:00

abstract：:Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 oper...

journal_title：Molecular microbiology

authors： Webster C,Merryweather A,Brammar W

更新日期：1992-02-01 00:00:00

abstract：:The F factor traY gene product (TraYp) is a site-specific DNA-binding protein involved in initiation of DNA transfer during bacterial conjugation. The sequence of TraYp exhibits a unique direct-repeat structure predicted to have a ribbon-helix-helix DNA-binding motif in each repeat unit. The stoichiometry of TraYp bin...

journal_title：Molecular microbiology

authors： Nelson WC,Matson SW

更新日期：1996-06-01 00:00:00

abstract：:A bioassay was developed to identify stimuli that promote the transcriptional induction of the algD operon for alginate biosynthesis in Pseudomonas aeruginosa. Strain PAO1 carried the algD promoter fused to a chloramphenicol acetyl-transferase cartridge (PalgD-cat), and > 50 compounds were tested for promoting chloram...

journal_title：Molecular microbiology

authors： Wood LF,Leech AJ,Ohman DE

更新日期：2006-10-01 00:00:00

abstract：:Burkholderia mallei, the aetiologic agent of glanders, causes a variety of illnesses in animals and humans ranging from occult infections to acute fulminating septicaemias. To better understand the role of lipopolysaccharide (LPS) in the pathogenesis of these diseases, studies were initiated to characterize the struct...

journal_title：Molecular microbiology

authors： Brett PJ,Burtnick MN,Snyder DS,Shannon JG,Azadi P,Gherardini FC

更新日期：2007-01-01 00:00:00

abstract：:During a normal cell cycle, chromosomes are exposed to many biochemical reactions that require specific types of DNA movement. Separation forces move replicated chromosomes into separate sister cell compartments during cell division, and the contemporaneous acts of DNA replication, RNA transcription and cotranscriptio...

journal_title：Molecular microbiology

authors： Deng S,Stein RA,Higgins NP

更新日期：2005-09-01 00:00:00

abstract：:RepA, the initiator protein of plasmid P1, binds to multiple sites (iterons) in the origin. The binding normally requires participation of chaperones, DnaJ, DnaK and GrpE. When purified, RepA appears dimeric and is inactive in iteron binding. On reaction with chaperones, a species active in iteron binding is formed an...

journal_title：Molecular microbiology

authors： Dibbens JA,Muraiso K,Chattoraj DK

更新日期：1997-10-01 00:00:00

abstract：:In response to genotoxic stress (GS), Candida albicans can undergo polarized growth and massive genome rearrangements including loss-of-heterozygosity (LOH) events. We evaluated the contribution of the CaRad53p and CaDun1p kinases of the DNA damage checkpoint (DDCP) in these processes. Characterization of C. albicans ...

journal_title：Molecular microbiology

authors： Loll-Krippleber R,d'Enfert C,Feri A,Diogo D,Perin A,Marcet-Houben M,Bougnoux ME,Legrand M

更新日期：2014-02-01 00:00:00

abstract：:The cell surface of Bacillus stearothermophilus PV72 is covered by a regular surface layer (S-layer) composed of single species of protein, SbsA, with a molecular weight of 130,000. Recently, the sequence of the corresponding gene (sbsA) has been determined. The SbsA coding region including the signal sequence was clo...

journal_title：Molecular microbiology

authors： Kuen B,Sára M,Lubitz W

更新日期：1996-02-01 00:00:00

abstract：:Restart of arrested replication forks is an important process and PriA, the main Escherichia coli replication restart protein, is essential for viability under any condition that increases the frequency of fork arrest. In priA mutant, replication forks are arrested by spontaneously occurring roadblocks and blocked rep...

journal_title：Molecular microbiology

authors： Grompone G,Sanchez N,Dusko Ehrlich S,Michel B

更新日期：2004-04-01 00:00:00

abstract：:The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...

journal_title：Molecular microbiology

authors： Ye D,Blanke SR

更新日期：2002-03-01 00:00:00

abstract：:The Bacillus thuringiensis insecticidal crystal protein CryIIA has both high mosquito activity and gypsy moth activity; in contrast CryIIB, which is 87% homologous, displays no mosquito activity and has a three-fold lower gypsy moth activity. The regions responsible for specificity against gypsy moth (Lymantria dispar...

journal_title：Molecular microbiology

authors： Liang Y,Dean DH

更新日期：1994-08-01 00:00:00

abstract：:Cytochrome b558 in the cytoplasmic membrane of Bacillus subtilis constitutes the anchor and electron acceptor to the flavoprotein (Fp) and iron-sulphur protein (Ip) in succinate:quinone oxidoreductase, and seemingly contains two haem groups. EPR and MCD spectroscopic data indicate bis-imidazole ligation of the haem. A...

journal_title：Molecular microbiology

authors： Fridén H,Hederstedt L

更新日期：1990-06-01 00:00:00

abstract：:NalC is a TetR type regulator that represses the multidrug efflux pump MexAB-OprM in Pseudomonas aeruginosa. Here we explain the mechanism of NalC-mediated regulation of MexAB-OprM. We show that NalC non-covalently binds chlorinated phenols and chemicals containing chlorophenol side-chains such as triclosan. NalC-chlo...

journal_title：Molecular microbiology

authors： Ghosh S,Cremers CM,Jakob U,Love NG

更新日期：2011-03-01 00:00:00

abstract：:Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of ...

journal_title：Molecular microbiology

authors： Monack DM,Arico B,Rappuoli R,Falkow S

更新日期：1989-12-01 00:00:00

abstract：:Each year, several million tons of dimethylsulfoniopropionate (DMSP) are produced by marine phytoplankton and bacteria as an important osmolyte to regulate their cellular osmosis. Microbial breakdown of DMSP to the volatile gas dimethylsulfide (DMS) plays an important role in global biogeochemical cycles of the sulphu...

journal_title：Molecular microbiology

authors： Chen Y,Schäfer H

更新日期：2019-06-01 00:00:00

abstract：:Protein translocation across the bacterial cytoplasmic membrane has been studied extensively in Escherichia coli. The identification of the components involved and subsequent reconstitution of the purified translocation reaction have defined the minimal constituents that allowed extensive biochemical characterization ...

journal_title：Molecular microbiology

authors： Manting EH,Driessen AJ

更新日期：2000-07-01 00:00:00

abstract：:The gene cbhA from the cellulolytic bacterium Cellulomonas fimi encodes a protein of 872 amino acids designated cellobiohydrolase A (CbhA). Mature CbhA contains 832 amino acid residues and has a predicted molecular mass of 85,349 Da. It is composed of five domains: an N-terminal catalytic domain, three repeated sequen...

journal_title：Molecular microbiology

authors： Meinke A,Gilkes NR,Kwan E,Kilburn DG,Warren RA,Miller RC Jr

更新日期：1994-05-01 00:00:00

abstract：:A unique 373 bp region (igr66) between grpE and dnaK of Streptococcus mutans lacks a promoter but is required for optimal production of DnaK. Northern blotting using probes specific to hrcA, igr66 or dnaK revealed multiple transcripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcr...

journal_title：Molecular microbiology

authors： Palmer SR,Burne RA

更新日期：2015-10-01 00:00:00

abstract：:The structural complexity of a ligand in association with the molecular chaperones SecB and SecA was investigated using three species of precursor maltose-binding protein, which differ in their stability as a result of an amino acid substitution in each that affects the rate of folding of the polypeptide. In the prese...

journal_title：Molecular microbiology

authors： de Cock H,Randall LL

更新日期：1998-01-01 00:00:00

abstract：:Biofilm formation in Bacillus subtilis requires expression of the eps and tapA-sipW-tasA operons to synthesize the extracellular matrix components, extracellular polysaccharide and TasA amyloid proteins, respectively. Expression of both operons is inhibited by the DNA-binding protein master regulator of biofilm format...

journal_title：Molecular microbiology

authors： Winkelman JT,Bree AC,Bate AR,Eichenberger P,Gourse RL,Kearns DB

更新日期：2013-06-01 00:00:00

abstract：:The inv locus of Yersinia enterocolitica is sufficient to convert a non-invasive Escherichia coli K12 strain into a microorganism that is able to penetrate cultured mammalian cells. The nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin...

journal_title：Molecular microbiology

authors： Young VB,Miller VL,Falkow S,Schoolnik GK

更新日期：1990-07-01 00:00:00