Abstract:
:The relF gene in Escherichia coli is related to the hok gene on plasmid R1. Both genes encode small proteins which, when overexpressed in E. coli lead to collapse of the membrane potential and cell death. A third gene, designated gef, which encodes a homologous cell-toxic protein, has been isolated from E. coli DNA. Both gef and relF are transcribed in E. coli and subject to post-transcriptional regulation which, in the case of gef, is coupled to translation of a leader sequence. The finding of homologous sequences in such distantly related bacteria as Agrobacterium and Rhizobium species suggests an important physiological role.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Poulsen LK,Larsen NW,Molin S,Andersson Pdoi
10.1111/j.1365-2958.1989.tb00131.xsubject
Has Abstractpub_date
1989-11-01 00:00:00pages
1463-72issue
11eissn
0950-382Xissn
1365-2958journal_volume
3pub_type
杂志文章abstract::In contrast to many type II restriction enzymes, dimeric proteins that cleave DNA at individual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to two copies of an elongated sequence before cutting the DNA at both sites. The mode of action of the SfiI endonuclease thus seems mor...
journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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更新日期:2007-12-01 00:00:00
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