Abstract:
:The Bacillus thuringiensis insecticidal crystal protein CryIIA has both high mosquito activity and gypsy moth activity; in contrast CryIIB, which is 87% homologous, displays no mosquito activity and has a three-fold lower gypsy moth activity. The regions responsible for specificity against gypsy moth (Lymantria dispar) and mosquito (Aedes aegypti) larvae were located by introducing MluI and XhoI sites into homologous positions within the putative domain II of both cryIIA and cryIIB genes, which divided almost equally the respective second domains into three regions. Taking advantage of naturally occurring NheI and NarI sites that border the putative domain II, a set of seven chimeric proteins were produced by exchanging all combinations of those regions between CryIIA and CryIIB. Analysis of the toxicity of these chimeric proteins demonstrated that the lepidopteran and dipteran specificity regions of CryIIA were not colinear. While the specificity region of CryIIA against mosquito larvae involved region 1 and probably also region 2, the specificity region of CryIIA against gypsy moth larvae was located within region 2.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Liang Y,Dean DHdoi
10.1111/j.1365-2958.1994.tb00451.xsubject
Has Abstractpub_date
1994-08-01 00:00:00pages
569-75issue
4eissn
0950-382Xissn
1365-2958journal_volume
13pub_type
杂志文章abstract::Colletotrichum lagenarium and Magnaporthe grisea are plant pathogenic fungi that produce melanin during the appressorial differentiation stage of conidial germination and during the late stationary phase of mycelial growth. Here, we report the identification of genes for two unique transcription factors, CMR1 (Colleto...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1995-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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更新日期:1994-02-01 00:00:00
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更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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更新日期:2011-03-01 00:00:00