Abstract:
:The dominant, polA1-independent replicon of pGSH500, rep beta (1.8 kb), consists of a cis-acting oriV region of 245 bp; a repB gene that is essential for autonomous replication and 18, 30 to 36 bp iterons which constitute the inc/cop region. The molecular organization of rep beta resembles that of mini-pCU1 (IncN). Furthermore, there is a 58% identity between the Rep proteins of these replicons. RepB also shows a 31% identity with RepE of mini-F. In addition, an 80% identity over 200 bp was identified between the cis-acting beta oriV region and the equivalent region of ori-2 (mini-F). Replicons with deletions of repB could be complemented by Rep (pCU1) and RepE (mini-F) in trans, supporting the hypothesis that rep beta is a natural hybrid between a pCU1-like and F-like replicon.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
da Silva-Tatley FM,Steyn LMdoi
10.1111/j.1365-2958.1993.tb01171.xsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
805-23issue
5eissn
0950-382Xissn
1365-2958journal_volume
7pub_type
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