Abstract:
:DNA processing reactions often involve multiple components acting in concert to achieve the desired outcome. However, it is usually difficult to know how the components communicate and cooperate to orchestrate an ordered series of events. We address this question in the context of the Tn 10 transposition reaction, in which the DNA cleavage and joining events occur within a higher-order complex containing a transposase dimer, two transposon ends and the DNA-bending host-factor IHF (Integration Host Factor). Previously it was shown that the complex is asymmetric. The a side consists of an IHF protomer initially immobilized by a DNA-loop, but subsequently used to promote conformational changes required for the cleavage steps. The beta side of the complex was considered to fulfil a more passive role. Here we show that the a side of the complex promotes coupled conformational changes at both transposon ends, while the a and beta sides communicate and cooperate to dominate different phases of the transposition reaction. Together, these effects provide for a robust response to critical changes in the transposon end. These findings also explain the intriguing genetic phenotypes of a series of previously reported Tn10 mutants and have consequences for the evolution of new elements.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Liu D,Sewitz S,Crellin P,Chalmers Rdoi
10.1111/j.1365-2958.2006.05432.xsubject
Has Abstractpub_date
2006-12-01 00:00:00pages
1522-33issue
6eissn
0950-382Xissn
1365-2958pii
MMI5432journal_volume
62pub_type
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