Transformation in Streptococcus pneumoniae: formation of eclipse complex in a coiA mutant implicates CoiA in genetic recombination.

abstract：:The repABC replicons contain an operon encoding the initiator protein (RepC) and partitioning proteins (RepA and RepB). The latter two proteins negatively regulate the transcription of the operon. In this article we have identified two novel regulatory elements, located within the conserved repB-repC intergenic sequen...

journal_title：Molecular microbiology

authors： Venkova-Canova T,Soberón NE,Ramírez-Romero MA,Cevallos MA

更新日期：2004-12-01 00:00:00

abstract：:The Pseudomonas aeruginosa protein PtxS negatively regulates its own synthesis by binding to the upstream region of its gene. We have recently identified a 14 bp palindromic sequence within the ptxS upstream region as the PtxS operator site (OP1). In this study, we searched the P. aeruginosa genomic sequence to determ...

journal_title：Molecular microbiology

authors： Swanson BL,Hager P,Phibbs P Jr,Ochsner U,Vasil ML,Hamood AN

更新日期：2000-08-01 00:00:00

abstract：:The H-NS protein of enteric bacteria is one of the major proteins of the bacterial nucleoid and seems to play an important role in nucleoid structure. Transcription of the hns gene encoding the H-NS protein appears to be negatively regulated by H-NS itself both in vitro and in vivo. We have examined the role of this m...

journal_title：Molecular microbiology

authors： Free A,Dorman CJ

更新日期：1995-10-01 00:00:00

abstract：:We report here the identification and characterization of a member of the Myxococcus xanthus SdeK signal transduction pathway, BrgE. This protein was identified as an SdeK-interacting component using a yeast two-hybrid screen, and we further confirmed this interaction by the glutathione S-transferase (GST) pulldown as...

journal_title：Molecular microbiology

authors： Pham VD,Shebelut CW,Zumstein EJ,Singer M

更新日期：2005-08-01 00:00:00

abstract：:Most available knowledge on fungal arginine metabolism is derived from studies on Saccharomyces cerevisiae, in which arginine catabolism is initiated by releasing urea via the arginase reaction. Orthologues of the S. cerevisiae genes encoding the first three enzymes in the arginase pathway were cloned from Kluyveromyc...

journal_title：Molecular microbiology

authors： Romagnoli G,Verhoeven MD,Mans R,Fleury Rey Y,Bel-Rhlid R,van den Broek M,Seifar RM,Ten Pierick A,Thompson M,Müller V,Wahl SA,Pronk JT,Daran JM

更新日期：2014-07-01 00:00:00

abstract：:A series of lipoproteins was detected in the membrane fraction of Yersinia enterocolitica W227, a typical strain from serotype O:9. At least two of them, YlpA and YlpB, are encoded by the pYV plasmid. The sequence of ylpA reveals the presence of a typical lipoprotein signal peptide. The mature YlpA protein would be 22...

journal_title：Molecular microbiology

authors： China B,Michiels T,Cornelis GR

更新日期：1990-09-01 00:00:00

abstract：:The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which ...

journal_title：Molecular microbiology

authors： Spiro S,Guest JR

更新日期：1988-11-01 00:00:00

abstract：:The translocation of proteins across the bacterial cell membrane is carried out by highly conserved components of the Sec system. Most bacterial species have a single copy of the genes encoding SecA and SecY, which are essential for viability. However, Streptococcus gordonii strain M99 encodes SecA and SecY homologues...

journal_title：Molecular microbiology

authors： Bensing BA,Sullam PM

更新日期：2002-05-01 00:00:00

abstract：:A new virus of halophilic Archaea is reported by Liu et al., and is remarkable in many ways. SNJ2 is the first temperate, pleomorphic virus (pleolipovirus) that integrates into the genome of its host. Analyses of the virus structure and its genome have provided an unexpected puzzle while at the same time solving anoth...

journal_title：Molecular microbiology

authors： Dyall-Smith M,Porter K

更新日期：2015-12-01 00:00:00

abstract：:Toxin-antitoxin loci have been identified in almost all free-living prokaryotes, often in high copy numbers. The biological function and molecular targets of the abundant vapBC loci are not yet known. Here we analyse the vapBC loci of Salmonella LT2 and Shigella plasmid pMYSH6000. Both loci encode putative PIN (PilT N...

journal_title：Molecular microbiology

authors： Winther KS,Gerdes K

更新日期：2009-05-01 00:00:00

abstract：:The 9.5 kb virB operon is the largest of the six major operons in the Ti plasmid vir region. This operon contains eleven genes, the largest of which is virB4. This gene encodes an 84 kDa protein whose function has not been identified. Its roles in conferring virulence on Agrobacterium tumefaciens and in the T-DNA tran...

journal_title：Molecular microbiology

authors： Shirasu K,Koukolíková-Nicola Z,Hohn B,Kado CI

更新日期：1994-02-01 00:00:00

abstract：:The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 ...

journal_title：Molecular microbiology

authors： Neale HC,Jackson RW,Preston GM,Arnold DL

更新日期：2018-11-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

journal_title：Molecular microbiology

authors： Hinnebusch J,Bergström S,Barbour AG

更新日期：1990-05-01 00:00:00

abstract：:Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these mole...

journal_title：Molecular microbiology

authors： Katz A,Elgamal S,Rajkovic A,Ibba M

更新日期：2016-08-01 00:00:00

abstract：:TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

journal_title：Molecular microbiology

authors： Larsen RA,Wood GE,Postle K

更新日期：1993-12-01 00:00:00

abstract：:DNA processing reactions often involve multiple components acting in concert to achieve the desired outcome. However, it is usually difficult to know how the components communicate and cooperate to orchestrate an ordered series of events. We address this question in the context of the Tn 10 transposition reaction, in ...

journal_title：Molecular microbiology

authors： Liu D,Sewitz S,Crellin P,Chalmers R

更新日期：2006-12-01 00:00:00

abstract：:The bacterium Streptomyces davaonensis produces the antibiotic roseoflavin, which is a riboflavin (vitamin B2 ) analog. The key enzyme of roseoflavin biosynthesis is the 8-demethyl-8-amino-riboflavin-5'-phosphate (AFP) synthase RosB which synthesizes AFP from riboflavin-5'-phosphate. AFP is not a substrate for the las...

journal_title：Molecular microbiology

authors： Schneider C,Konjik V,Kißling L,Mack M

更新日期：2020-10-01 00:00:00

abstract：:Escherichia coli has two heat shock regulons under the transcriptional control of Esigma32 and EsigmaE RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that...

journal_title：Molecular microbiology

authors： Klein G,Dartigalongue C,Raina S

更新日期：2003-04-01 00:00:00

abstract：:Most of the genes required for the conjugative transfer of DNA are encoded by the 33 kb transfer (tra) operon of F-like conjugative plasmids. Transcription of the tra operon is positively regulated by the TraJ transcriptional activator which, in turn, is negatively regulated by the FinOP fertility inhibition system. T...

journal_title：Molecular microbiology

authors： van Biesen T,Frost LS

更新日期：1994-11-01 00:00:00

abstract：:The product of the codY gene is required for nutritional repression of the Bacillus subtillis dipeptide permease operon (dpp), an operon expressed at early stationary phase in nutrient-rich medium. Though unrelated to any known DNA-binding protein, CodY was shown to bind specifically to the dpp promoter region. DNase ...

journal_title：Molecular microbiology

authors： Serror P,Sonenshein AL

更新日期：1996-05-01 00:00:00

abstract：:Bacterial chemoreceptors cluster into exquisitively sensitive, tunable, highly ordered, polar arrays. While these arrays serve as paradigms of cell signalling in general, it remains unclear what conformational changes transduce signals from the periplasmic tips, where attractants and repellents bind, to the cytoplasmi...

journal_title：Molecular microbiology

authors： Briegel A,Beeby M,Thanbichler M,Jensen GJ

更新日期：2011-11-01 00:00:00

abstract：:A 34 kb fragment of the Nocardia lactamdurans DNA carrying the cluster of early cephamycin biosynthetic genes was cloned in lambda EMBL3 by hybridization with probes internal to the pcbAB and pcbC genes of Penicillium chrysogenum and Streptomyces griseus. The pcbAB and pcbC genes were found to be closely linked togeth...

journal_title：Molecular microbiology

authors： Coque JJ,Martín JF,Calzada JG,Liras P

更新日期：1991-05-01 00:00:00

abstract：:The interactions between pathogenic bacteria and extracellular matrix (ECM) components markedly influence the initiation and establishment of infection. We have identified two surface proteins of virulent Mycoplasma pneumoniae with molecular masses of 45 and 30 kDa that bind to the ECM constituent, fibronectin (Fn). T...

journal_title：Molecular microbiology

authors： Dallo SF,Kannan TR,Blaylock MW,Baseman JB

更新日期：2002-11-01 00:00:00

abstract：:Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...

journal_title：Molecular microbiology

authors： Thomas JA,Weintraub ST,Wu W,Winkler DC,Cheng N,Steven AC,Black LW

更新日期：2012-04-01 00:00:00

abstract：:Biofilms are communities of microbial cells that are encased in a self-produced, polymeric matrix and are adherent to a surface. For several species of bacteria, an enhanced ability to form biofilms has been linked with an increased capability to produce exopolymers. To identify exopolymers of Bacillus subtilis that c...

journal_title：Molecular microbiology

authors： Stanley NR,Lazazzera BA

更新日期：2005-08-01 00:00:00

abstract：:Expression of the 987P gene cluster is activated by the adjacent IS1 element of an STpa transposon. Nucleotide sequence analysis of the 987P-DNA region contiguous with this IS1 element revealed the presence of an open reading frame designated fapR, encoding a basic protein of 260 amino acid residues with a molecular m...

journal_title：Molecular microbiology

authors： Klaasen P,de Graaf FK

更新日期：1990-10-01 00:00:00

abstract：:Glutathione (GSH) is an abundant low-molecular-mass thiol which has been implicated in numerous cellular processes including protection against cytotoxic agents such as xenobiotics, carcinogens and free radicals. Utilization of GSH results in its conversion to the oxidized form (GSSG), and it is recycled to GSH by the...

journal_title：Molecular microbiology

authors： Grant CM,Maciver FH,Dawes IW

更新日期：1996-11-01 00:00:00

abstract：:We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

journal_title：Molecular microbiology

authors： Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

更新日期：2004-01-01 00:00:00

abstract：:Viable mutations affecting the 5'-phosphate sensor of RNase E, including R169Q or T170A, become lethal when combined with deletions removing part of the non-catalytic C-terminal domain of RNase E. The phosphate sensor is required for efficient autoregulation of RNase E synthesis as RNase E R169Q is strongly overexpres...

journal_title：Molecular microbiology

authors： Garrey SM,Mackie GA

更新日期：2011-06-01 00:00:00

abstract：:The Streptomyces wild-type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5' ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0...

journal_title：Molecular microbiology

authors： Volff JN,Altenbuchner J

更新日期：1998-01-01 00:00:00