Roles of the 5'-phosphate sensor domain in RNase E.

abstract：:Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...

journal_title：Molecular microbiology

authors： Pagliarulo C,Salvatore P,De Vitis LR,Colicchio R,Monaco C,Tredici M,Talà A,Bardaro M,Lavitola A,Bruni CB,Alifano P

更新日期：2004-03-01 00:00:00

abstract：:A locust within chromosome XIII of Saccharomyces cerevisiae containing four genes upregulated by osmotic stress has been characterized. Two of the genes, but not their osmotic induction, were already described: the DNA damage-inducible gene DDR48 and the protease inhibitor gene PAI3. The two novel genes encode a cytop...

journal_title：Molecular microbiology

authors： Miralles VJ,Serrano R

更新日期：1995-08-01 00:00:00

abstract：:The two-component regulatory system PhoPQ has been shown to regulate the expression of virulence factors in a number of bacterial species. For one such virulence factor, lipopolysaccharide (LPS), the PhoPQ system has been shown to regulate structural modifications in Salmonella enterica var Typhimurium. In Yersinia pe...

journal_title：Molecular microbiology

authors： Hitchen PG,Prior JL,Oyston PC,Panico M,Wren BW,Titball RW,Morris HR,Dell A

更新日期：2002-06-01 00:00:00

abstract：:We cloned the CaYFH1 gene that encodes the yeast frataxin homologue in Candida albicans. CaYFH1 was expressed in Deltayfh1 Saccharomyces cerevisiae cells, where it compensated for all the phenotypes tested except for the lack of cytochromes. Double DeltaCayfh1/DeltaCayfh1 mutant had severe defective growth, accumulate...

journal_title：Molecular microbiology

authors： Santos R,Buisson N,Knight SA,Dancis A,Camadro JM,Lesuisse E

更新日期：2004-10-01 00:00:00

abstract：:Nitrogen-regulated genes in enterobacteria are positively controlled by the transcriptional activator of σ(N) -dependent promoters NtrC, either directly or indirectly, through the dual regulator Nac. Similar to enterobacteria, gdhA encoding glutamate dehydrogenase from Pseudomonas putida is one of the few genes that i...

journal_title：Molecular microbiology

authors： Hervás AB,Canosa I,Santero E

更新日期：2010-10-01 00:00:00

abstract：:MisL is an autotransporter protein encoded by Salmonella pathogenicity island 3 (SPI3). To investigate the role of MisL in Salmonella enterica serotype Typhimurium (S. Typhimurium) pathogenesis, we characterized its function during infection of mice and identified a host receptor for this adhesin. In a mouse model of ...

journal_title：Molecular microbiology

authors： Dorsey CW,Laarakker MC,Humphries AD,Weening EH,Bäumler AJ

更新日期：2005-07-01 00:00:00

abstract：:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medi...

journal_title：Molecular microbiology

authors： Repoila F,Gutierrez C

更新日期：1991-03-01 00:00:00

abstract：:All 21 of the Nudix hydrolase genes from the radiation-resistant organism Deinococcus radiodurans have been cloned into vectors under the control of T7 promoters and expressed as soluble proteins in Escherichia coli. Their sizes range from 9.8 kDa (91 amino acids) to 59 kDa (548 amino acids). Two novel proteins were i...

journal_title：Molecular microbiology

authors： Xu W,Shen J,Dunn CA,Desai S,Bessman MJ

更新日期：2001-01-01 00:00:00

abstract：:A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchron...

journal_title：Molecular microbiology

authors： Jensen RB,Grohmann E,Schwab H,Díaz-Orejas R,Gerdes K

更新日期：1995-07-01 00:00:00

abstract：:Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these mole...

journal_title：Molecular microbiology

authors： Katz A,Elgamal S,Rajkovic A,Ibba M

更新日期：2016-08-01 00:00:00

abstract：:Having no known environmental reservoir, Streptococcus pyogenes, a bacterium responsible for a wider variety of human diseases than any other bacterial species, must rely on its host for metabolic substrates. Although a streptococcal aldolase, LacD.1, has been adapted to virulence gene regulation, both LacD.1 and a pa...

journal_title：Molecular microbiology

authors： Loughman JA,Caparon MG

更新日期：2007-04-01 00:00:00

abstract：:Escherichia coli and Salmonella can use chitin-derived oligosaccharides as carbon and nitrogen sources. Chitosugars traverse the outer membrane through a dedicated chitoporin, ChiP, and are transported across the cytoplasmic membrane by the chitobiose transporter (ChbBCA). Previous work revealed that synthesis of the ...

journal_title：Molecular microbiology

authors： Plumbridge J,Bossi L,Oberto J,Wade JT,Figueroa-Bossi N

更新日期：2014-05-01 00:00:00

abstract：:Differential gene expression is a key strategy adopted by the Lyme disease spirochaete, Borrelia burgdorferi, for adaptation and survival in the mammalian host and the tick vector. Many B. burgdorferi surface lipoproteins fall into two distinct groups according to their expression patterns: one group primarily express...

journal_title：Molecular microbiology

authors： Wang P,Dadhwal P,Cheng Z,Zianni MR,Rikihisa Y,Liang FT,Li X

更新日期：2013-09-01 00:00:00

abstract：:The stable maintenance of low-copy-number plasmids requires active partitioning, with the most common mechanism in prokaryotes involving the ATPase ParA. ParA proteins undergo intricate spatiotemporal relocations across the nucleoid, dynamics that function to position plasmids at equally spaced intervals. This spacing...

journal_title：Molecular microbiology

authors： Howard M,Gerdes K

更新日期：2010-10-01 00:00:00

abstract：:The sequence of phi Ch1 contains an open reading frame (int1) in the central part of its genome that belongs to the lambda integrase family of site-specific recombinases. Sequence similarities to known integrases include the highly conserved tetrad R-H-R-Y. The flanking sequences of int1 contain several direct repeats...

journal_title：Molecular microbiology

authors： Rössler N,Klein R,Scholz H,Witte A

更新日期：2004-04-01 00:00:00

abstract：:GspB is a large cell-surface glycoprotein expressed by Streptococcus gordonii M99 that mediates binding of this organism to human platelets. This adhesin is glycosylated in the cytoplasm, and is then transported to the cell surface via an accessory Sec system. To assess the structural features of GspB that are needed ...

journal_title：Molecular microbiology

authors： Bensing BA,Takamatsu D,Sullam PM

更新日期：2005-12-01 00:00:00

abstract：:Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance. RnlA functions as a toxin with mRNA endoribonuclease activity and the cognate antitoxin RnlB inhibits RnlA toxicity in E. coli cells. Interestingly, T4 phage encodes the antitoxin Dmd, which acts...

journal_title：Molecular microbiology

authors： Wei Y,Gao ZQ,Otsuka Y,Naka K,Yonesaki T,Zhang H,Dong YH

更新日期：2013-12-01 00:00:00

abstract：:Thymidylate, an essential building block of DNA, is synthesized either from deoxyuridylate by thymidylate synthase (TS) or thymidine (dT) by thymidine kinase (TK). Thymidylate kinase (TMPK) phosphorylates dTMP to dTTP. Thymidine phosphorylase (TP) catalyses reversible phosphorolysis of dT. Using transposon mutagenesis...

journal_title：Molecular microbiology

authors： Wang L,Hames C,Schmidl SR,Stülke J

更新日期：2010-09-01 00:00:00

abstract：:Multicopy dnaA(Ts) strains carrying the dnaA5 or dnaA46 allele are high-temperature resistant but are cold sensitive for colony formation. The DnaA5 and DnaA46 proteins both have an A184-->V change in the ATP binding motif of the protein, but they also have one additional mutation. The mutations were separated, and it...

journal_title：Molecular microbiology

authors： Nyborg M,Atlung T,Skovgaard O,Hansen FG

更新日期：2000-03-01 00:00:00

abstract：:Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...

journal_title：Molecular microbiology

authors： Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

更新日期：2002-11-01 00:00:00

abstract：:Expression of Escherichia coli hupA and hupB, the structural genes encoding the most abundant nucleoid-associated proteins HUalpha and HUbeta has been studied during cold shock. This article demonstrates that: (i) transcriptional expression of hupA is blocked following a sudden temperature downshift (from 37 degrees C...

journal_title：Molecular microbiology

authors： Giangrossi M,Giuliodori AM,Gualerzi CO,Pon CL

更新日期：2002-04-01 00:00:00

abstract：:In almost all bacteria, cell division is co-ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential to yield an important new class of antibiotic. However, the evo...

journal_title：Molecular microbiology

authors： Adams DW,Wu LJ,Errington J

更新日期：2016-03-01 00:00:00

abstract：:The HPr kinase of Gram-positive bacteria is an ATP-dependent serine protein kinase, which phosphorylates the HPr protein of the bacterial phosphotransferase system (PTS) and is involved in the regulation of carbohydrate metabolism. The hprK gene from Enterococcus faecalis was cloned via polymerase chain reaction (PCR)...

journal_title：Molecular microbiology

authors： Kravanja M,Engelmann R,Dossonnet V,Blüggel M,Meyer HE,Frank R,Galinier A,Deutscher J,Schnell N,Hengstenberg W

更新日期：1999-01-01 00:00:00

abstract：SUMMARY:The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated b...

journal_title：Molecular microbiology

authors： Davidov E,Kaufmann G

更新日期：2008-09-01 00:00:00

abstract：:Although the aerobic degradation of aromatic compounds has been extensively studied in many microorganisms, the anaerobic mineralization of the aromatic ring is a more recently discovered microbial capacity on which very little information is available from facultative anaerobic bacteria. In this issue of Molecular Mi...

journal_title：Molecular microbiology

authors： Carmona M,Díaz E

更新日期：2005-12-01 00:00:00

abstract：:Aflatoxins are polyaromatic mycotoxins that contaminate a range of food crops as a result of fungal growth and contribute to serious health problems in the developing world because of their toxicity and mutagenicity. Although relatively resistant to biotic degradation, aflatoxins can be metabolized by certain species ...

journal_title：Molecular microbiology

authors： Taylor MC,Jackson CJ,Tattersall DB,French N,Peat TS,Newman J,Briggs LJ,Lapalikar GV,Campbell PM,Scott C,Russell RJ,Oakeshott JG

更新日期：2010-11-01 00:00:00

abstract：:Escherichia coli cells use two distinct sensory circuits during chemotaxis towards carbohydrates. One circuit requires the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and is independent of any specific chemoreceptor, whereas the other uses a chemoreceptor-dependent sensory mechanism analogous to that...

journal_title：Molecular microbiology

authors： Kristich CJ,Glekas GD,Ordal GW

更新日期：2003-03-01 00:00:00

abstract：:Chemotactic adaptation to persisting stimulation involves reversible methylation of the chemoreceptors that form complexes with the histidine kinase CheA at a cell pole. The methyltransferase CheR targets to the C-terminal NWETF sequence of the chemoreceptor. In contrast, localization of the methylesterase CheB is lar...

journal_title：Molecular microbiology

authors： Banno S,Shiomi D,Homma M,Kawagishi I

更新日期：2004-08-01 00:00:00

abstract：:Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutan...

journal_title：Molecular microbiology

authors： Ramey BE,Matthysse AG,Fuqua C

更新日期：2004-06-01 00:00:00

abstract：:Plasmodium parasites possess a single pyruvate dehydrogenase (PDH) enzyme complex that is localized to the plastid-like organelle known as the apicoplast. Unlike most eukaryotes, Plasmodium parasites lack a mitochondrial PDH. The PDH complex catalyses the conversion of pyruvate to acetyl-CoA, an important precursor fo...

journal_title：Molecular microbiology

authors： Pei Y,Tarun AS,Vaughan AM,Herman RW,Soliman JM,Erickson-Wayman A,Kappe SH

更新日期：2010-02-01 00:00:00