A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress.

abstract：:Our understanding of how obligate intracellular pathogens co-opt eukaryotic cellular functions has been limited by their intractability to genetic manipulation and by the abundance of pathogen-specific genes with no known functional homologues. In this report we describe a gene expression system to characterize protei...

journal_title：Molecular microbiology

authors： Sisko JL,Spaeth K,Kumar Y,Valdivia RH

更新日期：2006-04-01 00:00:00

abstract：:When fungi grow on plant or insect surfaces coated with wax polyesters that protect against pathogens, the fungi generally form aerial hyphae to contact the surfaces. Aerial structures such as hyphae and conidiophores are coated with hydrophobins, which are surface-active proteins involved in adhesion to hydrophobic s...

journal_title：Molecular microbiology

authors： Takahashi T,Maeda H,Yoneda S,Ohtaki S,Yamagata Y,Hasegawa F,Gomi K,Nakajima T,Abe K

更新日期：2005-09-01 00:00:00

abstract：:The replication frequency of plasmid R1 is controlled by an unstable antisense RNA, CopA, which, by binding to its complementary target, blocks translation of the replication rate-limiting protein RepA. Since the degree of inhibition is directly correlated with the intracellular concentration of CopA, factors affectin...

journal_title：Molecular microbiology

authors： Söderbom F,Binnie U,Masters M,Wagner EG

更新日期：1997-11-01 00:00:00

abstract：:Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated b...

journal_title：Molecular microbiology

authors： Kessler RL,Contreras VT,Marliére NP,Aparecida Guarneri A,Villamizar Silva LH,Mazzarotto GACA,Batista M,Soccol VT,Krieger MA,Probst CM

更新日期：2017-06-01 00:00:00

abstract：:Photorhabdus and Xenorhabdus bacteria colonize the intestines of the infective soil-dwelling stage of entomophagous nematodes, Heterorhabditis and Steinernema, respectively. These nematodes infect susceptible insect larvae and release the bacteria into the insect blood. The bacteria kill the insect larvae and convert ...

journal_title：Molecular microbiology

authors： Goodrich-Blair H,Clarke DJ

更新日期：2007-04-01 00:00:00

abstract：:In contrast to many type II restriction enzymes, dimeric proteins that cleave DNA at individual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to two copies of an elongated sequence before cutting the DNA at both sites. The mode of action of the SfiI endonuclease thus seems mor...

journal_title：Molecular microbiology

authors： Bilcock DT,Halford SE

更新日期：1999-02-01 00:00:00

abstract：:The kilB locus (which is unclonable in the absence of korB) of broad-host-range plasmid RK2 (60 kb) lies between the trfA operon (co-ordinates 16.4 to 18.2 kb), which encodes a protein essential for vegetative replication, and the Tra2 block of conjugative transfer genes (co-ordinates 20.0 to 27.0 kb). Promoter probe ...

journal_title：Molecular microbiology

authors： Motallebi-Veshareh M,Balzer D,Lanka E,Jagura-Burdzy G,Thomas CM

更新日期：1992-04-01 00:00:00

abstract：:Plasmodium parasites possess a single pyruvate dehydrogenase (PDH) enzyme complex that is localized to the plastid-like organelle known as the apicoplast. Unlike most eukaryotes, Plasmodium parasites lack a mitochondrial PDH. The PDH complex catalyses the conversion of pyruvate to acetyl-CoA, an important precursor fo...

journal_title：Molecular microbiology

authors： Pei Y,Tarun AS,Vaughan AM,Herman RW,Soliman JM,Erickson-Wayman A,Kappe SH

更新日期：2010-02-01 00:00:00

abstract：:Natural sequence variation was investigated among serogroup A subgroup IV-1 Neisseria meningitidis isolated from diseased patients and healthy carriers in The Gambia, West Africa. The frequencies of DNA import were analysed by sequencing fragments of four linked genes encoding the immunogenic outer membrane proteins T...

journal_title：Molecular microbiology

authors： Linz B,Schenker M,Zhu P,Achtman M

更新日期：2000-06-01 00:00:00

abstract：:Campylobacter jejuni, a prevalent cause of bacterial gastroenteritis, must adapt to different environments to be a successful pathogen. We previously identified a C. jejuni two-component regulatory system (Cj1226/7c) as upregulated during cell infections. Analyses described herein led us to designate the system CprRS ...

journal_title：Molecular microbiology

authors： Svensson SL,Davis LM,MacKichan JK,Allan BJ,Pajaniappan M,Thompson SA,Gaynor EC

更新日期：2009-01-01 00:00:00

abstract：:Bacillus subtilis forms long chains of cells during growth and biofilm formation. Cell separation is mediated by autolysins, whose genes are under the negative control of a heteromeric complex composed of the proteins SinR and SlrR. Formation of the SinR-SlrR complex is governed by a self-reinforcing, double-negative ...

journal_title：Molecular microbiology

authors： Chai Y,Kolter R,Losick R

更新日期：2010-10-01 00:00:00

abstract：:Wolinella succinogenes can grow by anaerobic respiration with nitrate or nitrite using formate as electron donor. Two forms of nitrite reductase were isolated from the membrane fraction of W. succinogenes. One form consisted of a 58 kDa polypeptide (NrfA) that was identical to the periplasmic nitrite reductase. The ot...

journal_title：Molecular microbiology

authors： Simon J,Gross R,Einsle O,Kroneck PM,Kröger A,Klimmek O

更新日期：2000-02-01 00:00:00

abstract：:Calcineurin phosphatase and cyclophilin A are cellular components involved in fungal morphogenesis and virulence. Their roles were investigated in the phytopathogenic fungus Botrytis cinerea using gene inactivation, drug inhibition and cDNA macroarrays approaches. First, the BCP1 gene coding for cyclophilin A was iden...

journal_title：Molecular microbiology

authors： Viaud M,Brunet-Simon A,Brygoo Y,Pradier JM,Levis C

更新日期：2003-12-01 00:00:00

abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...

journal_title：Molecular microbiology

authors： Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jr

更新日期：1995-06-01 00:00:00

abstract：:Genes acrAB encode a multidrug efflux pump in Escherichia coli. We have previously reported that transcription of acrAB is increased under general stress conditions (i.e. 4% ethanol, 0.5 M NaCl, and the stationary phase in Luria-Bertani medium). In this study, lacZ transcriptional fusions and an in vitro gel mobility ...

journal_title：Molecular microbiology

authors： Ma D,Alberti M,Lynch C,Nikaido H,Hearst JE

更新日期：1996-01-01 00:00:00

abstract：:In Aspergillus nidulans the global regulatory gene veA is necessary for the biosynthesis of several secondary metabolites, including the mycotoxin sterigmatocystin (ST). In order to identify additional veA-dependent genetic elements involved in regulating ST production, we performed a mutagenesis on a deletion veA (Δv...

journal_title：Molecular microbiology

authors： Ramamoorthy V,Shantappa S,Dhingra S,Calvo AM

更新日期：2012-08-01 00:00:00

abstract：:On the basis of the published N-terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein-processing site, was shown ...

journal_title：Molecular microbiology

authors： Ehlert K,Höltje JV,Templin MF

更新日期：1995-05-01 00:00:00

abstract：:The unfolded protein response (UPR) is an important stress signalling pathway involved in the cellular development and environmental adaptation of fungi. We investigated the importance of the UPR pathway in the pathogenicity of the plant necrotrophic fungus Alternaria brassicicola, which causes black spot disease on a...

journal_title：Molecular microbiology

authors： Joubert A,Simoneau P,Campion C,Bataillé-Simoneau N,Iacomi-Vasilescu B,Poupard P,François JM,Georgeault S,Sellier E,Guillemette T

更新日期：2011-03-01 00:00:00

abstract：:A new class of Bacillus thuringiensis delta-endotoxins, or insecticidal control proteins (ICPs), is defined by an apparently cryptic protein with a unique primary structure and novel entomocidal specificity for certain coleopteran and lepidopteran species. The discovery of a new group of ICPs will extend the use of th...

journal_title：Molecular microbiology

authors： Tailor R,Tippett J,Gibb G,Pells S,Pike D,Jordan L,Ely S

更新日期：1992-05-01 00:00:00

abstract：:The heptacistronic dnaK heat shock operon of Bacillus subtilis consists of the genes hrcA, grpE, dnaK, dnaJ, orf35, orf28 and orf50. It is controlled by the CIRCE/HrcA operator/repressor system and specifies three primary transcripts, two of which are processed into three different products. We have analysed the regul...

journal_title：Molecular microbiology

authors： Homuth G,Mogk A,Schumann W

更新日期：1999-06-01 00:00:00

abstract：:We have followed the time-dependent transfection of Paramecium cells with a vector containing the gene of green fluorescent protein (GFP) attached to the C-terminus of the PtSERCA1 gene. The outlines of alveolar sacs (ASs) are labelled, as is the endoplasmic reticulum (ER) throughout the cell. When GFP fluorescence is...

journal_title：Molecular microbiology

authors： Hauser K,Pavlovic N,Klauke N,Geissinger D,Plattner H

更新日期：2000-08-01 00:00:00

abstract：:The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

journal_title：Molecular microbiology

authors： Outten FW,Djaman O,Storz G

更新日期：2004-05-01 00:00:00

abstract：:The human oral bacterium Streptococcus gordonii expresses, on the cell surface, two antigenically related high-molecular-mass polypeptides denoted CshA and CshB, encoded by genes at separate chromosomal loci. The precursor form of CshA is composed of four distinct segments: (i) a 41-amino-acid residue leader peptide, ...

journal_title：Molecular microbiology

authors： McNab R,Jenkinson HF,Loach DM,Tannock GW

更新日期：1994-11-01 00:00:00

abstract：:Gene expression in the Leishmania is controlled post-transcriptionally, and is likely to be impacted by both 5' and 3' untranslated regions (UTRs). We have investigated the effects of trinucleotides in the AUG-proximal region (APR) (i.e. positions -3 to -1 upstream of an AUG) on two reporter genes in the context of an...

journal_title：Molecular microbiology

authors： Stanton JD,Mensa-Wilmot K

更新日期：2006-08-01 00:00:00

abstract：:Salmonella enterica subspecies I serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them to persist in this niche have been identified. We show that STM0557, a S. enterica subspecies I-specific gene encoding an inner membrane ...

journal_title：Molecular microbiology

authors： Bogomolnaya LM,Santiviago CA,Yang HJ,Baumler AJ,Andrews-Polymenis HL

更新日期：2008-12-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

journal_title：Molecular microbiology

authors： Ortega DR,Kjaer A,Briegel A

更新日期：2020-09-01 00:00:00

abstract：:Mutants (glk) of Streptomyces coelicolor A3(2) that are resistant to the non-utilizable glucose analogue 2-deoxyglucose are deficient in glucose kinase activity, defective in glucose repression, and usually unable to utilize glucose. A 2.9 kb BclI fragment, previously shown to restore a wild-type phenotype to a glk de...

journal_title：Molecular microbiology

authors： Angell S,Schwarz E,Bibb MJ

更新日期：1992-10-01 00:00:00

abstract：:The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine th...

journal_title：Molecular microbiology

authors： Parveen N,Cornell KA

更新日期：2011-01-01 00:00:00

abstract：:The streptococcal antigen I/II (AgI/II)-family polypeptides are cell wall-anchored adhesins expressed by most indigenous oral streptococci. Proteins sharing 30-40% overall amino acid sequence similarities with AgI/II-family proteins are also expressed by Streptococcus pyogenes. The S. pyogenes M28_Spy1325 polypeptide ...

journal_title：Molecular microbiology

authors： Maddocks SE,Wright CJ,Nobbs AH,Brittan JL,Franklin L,Strömberg N,Kadioglu A,Jepson MA,Jenkinson HF

更新日期：2011-08-01 00:00:00

abstract：:The pathogenicity of Plasmodium falciparum is partly due to parasite-induced host cell modifications. These modifications are facilitated by exported P. falciparum proteins, collectively referred to as the exportome. Export of several hundred proteins is mediated by the PEXEL/HT, a protease cleavage site. The PEXEL/HT...

journal_title：Molecular microbiology

authors： Schulze J,Kwiatkowski M,Borner J,Schlüter H,Bruchhaus I,Burmester T,Spielmann T,Pick C

更新日期：2015-07-01 00:00:00